Enrichment of Cancer Stem Cells Based on Heterogeneity of Invasiveness
- 338 Downloads
Cancer stem cells (CSCs) have multiple potentials in carcinogenesis and tumor progression. However, it is rather difficult to enrich and amplify CSCs either from tumor cell lines or even primary tumor tissues. Therefore, establishing new methodologies for isolation and enrichment based on the functional properties of CSCs is of great importance for studies on CSCs. According to the findings that CSCs possess more infiltrative capability as compared with their differentiated descendants, we propose a novel strategy based on heterogeneity of cancer cell invasiveness for isolation and enrichment of CSCs from committed cancer cell population. In addition, we hypothesize that existence of CSCs might be the real root of tumor invasion and metastasis.
KeywordsCancer stem cell Invasiveness Isolation Migration Extracellular matrix
This study was supported by grants from the National Basic Research Program of China (973 Program, No.2006CB708503) and the National Natural Science Foundation of China (No. 30725035, 30700863).
- 6.Van, S. A., Van der Pol, M. A., Kok, A., et al. (2003). Differences between the CD34+ and CD34− blast compartments in apoptosis resistance in acute myeloid leukemia. Haematologica, 88, 497–508.Google Scholar
- 10.Baba, T., Convery, P. A., Matsumura, N., et al. (2008). Epigenetic regulation of CD133 and tumorigenicity of CD133+ ovarian cancer cells. Oncogene. doi: 10.1038/onc.2008.374.
- 18.Patrawala, L., Calhoun, T., Schneider-Broussard, R., Zhou, J., Claypool, K., & Tang, D. G. (2005). Side population is enriched in tumorigenic, stem-Like cancer cells, whereas ABCG2+ and ABCG2− cancer cells are similarly tumorigenic. Cancer Research, 65, 6207–6219. doi: 10.1158/0008-5472.CAN-05-0592.PubMedCrossRefGoogle Scholar
- 19.Hirschmann Jax, C., Foster, A. E., Wulf, G. G., et al. (2004). A distinct “side population” of cells with high drug efflux capacity in human tumor cells. Proceedings of the National Academy of Sciences of the United States of America, 101, 14228–14233. doi: 10.1073/pnas.0400067101.PubMedCrossRefGoogle Scholar
- 31.Pellegatta, S., Poliani, P. L., Corno, D., et al. (2006). Neurospheres enriched in cancer stem-like cells are highly effective in eliciting a dendritic cell-mediated immune response against malignant gliomas. Cancer Research, 66, 10247–10252. doi: 10.1158/0008-5472.CAN-06-2048.PubMedCrossRefGoogle Scholar
- 36.Lee, J., Kotliarova, S., Kotliarov, Y., et al. (2006). Tumor stem cells derived from glioblastomas cultured in bFGF and EGF more closely mirror the phenotype and genotype of primary tumors than do serum-cultured cell lines. Cancer Cell, 9, 391–403. doi: 10.1016/j.ccr.2006.03.030.PubMedCrossRefGoogle Scholar