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Cell Biochemistry and Biophysics

, Volume 77, Issue 4, pp 367–377 | Cite as

Grape Seed Procyanidins Attenuates Cisplatin-induced Human Embryonic Renal Cell Cytotoxicity by Modulating Heme Oxygenase-1 in Vitro

  • Hedan Han
  • Hai Wang
  • Yuemei Du
  • Liping GaoEmail author
Original Paper
  • 49 Downloads

Abstract

Cisplatin is a widely used anti-cancer drug. However, cisplatin is limited in clinical treatment because of its severe nephrotoxicity. This study reported whether O-GSP can antagonize the cisplatin-induced cytotoxicity in HEK293 cells through inducing HO-1 protein expression. We previously demonstrated O-GSP can increase the survival rate of HEK293 and have protective effect on HEK293 cells. Herein, We found that O-GSP can antagonize cisplatin nephrotoxicity through regulating the expression of HO-1. O-GSP promotes the translocation of Nrf2 in the nucleus, and activates the ERKN JNK pathway and p38 MAPK pathway. Interestingly, p38 MAPK plays a major role in HO-1 expression induced by O-GSP. And O-GSP can modulate the decrease of Nrf2 and HO-1 expression induced by cisplatin, and improve the cisplatin-induced activity and apoptosis rate of cells by stimulating the expression of HO-1. However, the protective effects of O-GSP are inhibited by ZnPP IX. Collectively, the results indicated that O-GSP induced the expression of HO-1 through p38MAPK and Nrf2 pathway in HEK293 cells.

Keywords

Oligomeric grape seed procyanidins Cisplatin HO-1 Nrf2 Nephrotoxicity 

Notes

Acknowledgements

This work project is founded by grants from Scientific Research Project of Beijing Union University: Opening Project of Key Laboratory (NO.LDSP201801).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Hedan Han
    • 1
    • 2
  • Hai Wang
    • 1
    • 2
  • Yuemei Du
    • 1
    • 2
  • Liping Gao
    • 1
    • 2
    Email author
  1. 1.College of Biochemical EngineeringBeijing Union UniversityBeijingChina
  2. 2.Beijing Municipal Key Laboratory of Biologically Active Substances and Functional FoodBeijingChina

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