Dose-dependent 60Co γ-radiation Effects on Human Endothelial Cell Mechanical Properties
- 31 Downloads
Exposure to ionizing radiation is unavoidable for noncancerous cells during the external radiotherapy process. Increasing the dose delivery fraction times leads to increasing the endothelial cell damage. Vascular abnormalities are commonly associated with the alternation of endothelium biomechanical properties. The goal of the present study was to quantify the elastic and viscoelastic properties of human umbilical vein endothelial cells (HUVECs) using the micropipette aspiration technique in conjunction with a theoretical model while an 8 Gy dose was given in four fractions. Confocal imaging was performed for evaluation of cytoskeletal changes during fractionation 60Co radiotherapy. The results indicated an increase in elastic modulus from 29.87 ± 1.04 Pa to 46.69 ± 1.17 Pa while the fractional doses increased from 0 Gy to 8 Gy along with the obvious cytoskeletal changes. Moreover, in the creep behavior of radiated groups, a significant decrease was shown in the time constant and viscoelastic properties. On the other hand, it was observed that the change in the biomechanical properties of the cells while applying a single fraction of 8 Gy was not exactly the same as that in the properties of the radiation-exposed cells while delivering an 8 Gy dose at 2 Gy per fraction. The observed differences in the biomechanical behavior of endothelium provide a quantitative description of radiobiological effects for evaluating the dose-response relationship as a biological dosimetry procedure.
KeywordsFractionation radiotherapy Micropipette aspiration Confocal imaging Dose-response Endothelial cells
This study was approved by Tarbiat Modares University. This work was supported in part by the Iran National Science Foundation (INSF).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 4.Ren, X., Wang, Q., Zhang, R., Chen, X., Wang, N., Liu, Y., Zong, J., Guo, Z., Wang, D., & Lin, Q. (2016). Accelerated hypofractionated three-dimensional conformal radiation therapy (3 Gy/fraction) combined with concurrent chemotherapy for patients with unresectable stage III non-small cell lung cancer: Preliminary results of an early terminated phase II trial. BMC Cancer, 16, 1–13.CrossRefGoogle Scholar
- 6.Travis, L. B., Ng, A. K., Allan, J. M., Pui, C., Kennedy, A. R., Xu, X. G., Purdy, J. A., Applegate, K., Yahalom, J., Constine, L. S., Gilbert, E. S., & Boice, Jr., J. D. (2012). Second malignant neoplasms and cardiovascular disease following radiotherapy. Journal of the National Cancer Institute, 104, 357–370.PubMedPubMedCentralCrossRefGoogle Scholar
- 9.Bischof, M., Abdollahi, A., Gong, P., Stoffregen, C., Lipson, K. E., Debus, J., Weber, K. J., & Huber, P. E. (2004). The triple combination of irradiation, chemotherapy (pemetrexed), and VEGFR2 in human endothelial and tumor cells. International Journal of Radiation Oncology, Biology, Physics, 60, 1220–1232.PubMedCrossRefGoogle Scholar
- 11.Martin, J. D., Buckley, A. R., Graeb, D., Walman, B., Salvain, A., Hsy, J. H., & Chir, M. B. B. (2005). Carotid artery stenosis in asymptomatic patients who have received unilateral head-and-neck irradiation. International Journal of Radiation Oncology, Biology, Physics, 63, 1197–1205.PubMedCrossRefGoogle Scholar
- 13.Zhang, B., Liu, B., Zhang, H., & Wang, J. (2014). Erythrocyte stiffness during morphological remodeling induced by carbon ion radiation. PLoS ONE, 9, 1–19.Google Scholar
- 14.Zheng, Q., Liu, Y., Zhou, H. J., Du, Y. T., Zhang, B. P., Zhang, J., Miao, G. Y., Liu, B., & Zhang, H. (2015). X-ray radiation promotes the metastatic potential of tongue squamous cell carcinoma cells via modulation of biomechanical and cytoskeletal properties. Human & Experimental Toxicology, 34, 894–903.CrossRefGoogle Scholar
- 16.Pachenari, M., Seyedpour, S. M., Janmaleki, M., Babazadeh, S., Taranejoo, S., & Hosseinkhani, H. (2014). Mechanical properties of cancer cytoskeleton depend on actin filaments to microtubules content: Investigating different grades of colon cancer cell lines. Journal of Biomechanics, 47, 373–379.PubMedCrossRefGoogle Scholar
- 27.Wang, Z., Zhao, Z., Lu, J., Chen, Z., Mao, A., Teng, G., & Liu, F. (2015). A comparison of biological effects of 125I seeds continuous low-dose-rate radiation and 60Co high-dose-rate gamma radiation on non-small cell lung cancer cells. PLoS ONE, 10, 1–11.Google Scholar
- 31.Masoumi, H., Mokhtari-Dizaji, M., Arbabi, A., & Bakhshandeh, M. (2014). Determine the dose distribution using ultrasound parameters in MAGIC-f polymer gels. Dose Response, 13, 1–7.Google Scholar