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Dose-dependent 60Co γ-radiation Effects on Human Endothelial Cell Mechanical Properties

  • Alireza Mohammadkarim
  • Manijhe Mokhtari-DizajiEmail author
  • Ali Kazemian
  • Hazhir Saberi
  • Mohammad Mehdi Khani
  • Mohsen Bakhshandeh
Original Paper
  • 31 Downloads

Abstract

Exposure to ionizing radiation is unavoidable for noncancerous cells during the external radiotherapy process. Increasing the dose delivery fraction times leads to increasing the endothelial cell damage. Vascular abnormalities are commonly associated with the alternation of endothelium biomechanical properties. The goal of the present study was to quantify the elastic and viscoelastic properties of human umbilical vein endothelial cells (HUVECs) using the micropipette aspiration technique in conjunction with a theoretical model while an 8 Gy dose was given in four fractions. Confocal imaging was performed for evaluation of cytoskeletal changes during fractionation 60Co radiotherapy. The results indicated an increase in elastic modulus from 29.87 ± 1.04 Pa to 46.69 ± 1.17 Pa while the fractional doses increased from 0 Gy to 8 Gy along with the obvious cytoskeletal changes. Moreover, in the creep behavior of radiated groups, a significant decrease was shown in the time constant and viscoelastic properties. On the other hand, it was observed that the change in the biomechanical properties of the cells while applying a single fraction of 8 Gy was not exactly the same as that in the properties of the radiation-exposed cells while delivering an 8 Gy dose at 2 Gy per fraction. The observed differences in the biomechanical behavior of endothelium provide a quantitative description of radiobiological effects for evaluating the dose-response relationship as a biological dosimetry procedure.

Keywords

Fractionation radiotherapy Micropipette aspiration Confocal imaging Dose-response Endothelial cells 

Notes

Acknowledgements

This study was approved by Tarbiat Modares University. This work was supported in part by the Iran National Science Foundation (INSF).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Alireza Mohammadkarim
    • 1
  • Manijhe Mokhtari-Dizaji
    • 1
    Email author
  • Ali Kazemian
    • 2
  • Hazhir Saberi
    • 3
  • Mohammad Mehdi Khani
    • 4
  • Mohsen Bakhshandeh
    • 5
  1. 1.Department of Medical Physics, Faculty of Medical SciencesTarbiat Modares UniversityTehranIran
  2. 2.Radiation Oncology Research Center, Cancer InstituteTehran University of Medical SciencesTehranIran
  3. 3.Department of Radiology, Imam Khomeini HospitalTehran University of Medical SciencesTehranIran
  4. 4.Department of Tissue Engineering and Regenerative Medicine, School of Advanced Technologies in MedicineShahid Beheshti University of Medical SciencesTehranIran
  5. 5.Department of Radiology Technology, Faculty of Paramedical SciencesShahid Beheshti University of Medical Sciences and Health ServicesTehranIran

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