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Cell Biochemistry and Biophysics

, Volume 74, Issue 3, pp 435–447 | Cite as

Anti- and Pro-apoptotic Bcl2 Proteins Distribution and Metabolic Profile in Human Coronary Aorta Endothelial Cells Before and After HypPDT

  • Mária Maslaňáková
  • Lucia Balogová
  • Pavol Miškovský
  • Ružena Tkáčová
  • Katarína ŠtroffekováEmail author
Original Paper

Abstract

Understanding apoptosis regulatory mechanisms in endothelial cells (ECs) has great importance for the development of novel therapy strategies for cancer and cardiovascular pathologies. An oxidative stress with the generation of reactive oxygen species (ROS) is a common mechanism causing ECs’ dysfunction and apoptosis. The generation of ROS can be triggered by various stimuli including photodynamic therapy (PDT). In most PDT treatments, photosensitizer (PS) is administered systemically, and thus, possibility of high exposure to PS in the ECs remains high. PS accumulation in ECs may be clinically relevant even without PDT, if PS molecules affect the pro-apoptotic cascade without illumination. In the present work, we focused on Hypericin (Hyp) and HypPDT effects on the cell viability, oxidative stress, and the distribution of Bcl2 family members in human coronary artery endothelial (HCAEC) cells. Our findings show that the presence of Hyp itself has an effect on cell viability, oxidative stress, and the distribution of Bcl2 family members, without affecting the mitochondria function. In contrast, HypPDT resulted in mitochondria dysfunction, further increase of oxidative stress and effect on the distribution of Bcl2 family members, and in primarily necrotic type of death in HCAEC cells.

Keywords

Apoptosis Oxidative stress Hypericin Photodynamic therapy Bcl2 family of proteins 

Notes

Acknowledgments

Supported by the EU 7FP grant PIRG06-GA-2009-256580 and by EU Structural Fund ITMS26110230013; by the Slovak Grant Agency VEGA -1-0111-12 and VEGA 1/0425/15; and by the Slovak Research and Development Agency APVV-0134-11.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Mária Maslaňáková
    • 1
  • Lucia Balogová
    • 1
  • Pavol Miškovský
    • 1
    • 2
  • Ružena Tkáčová
    • 3
  • Katarína Štroffeková
    • 1
    Email author
  1. 1.Department of Biophysics, Faculty of Natural SciencesPJ Safarik UniversityKosiceSlovakia
  2. 2.Center of Interdisciplinary Biosciences, Faculty of Natural SciencesPJ Safarik UniversityKosiceSlovakia
  3. 3.Department of Respiratory Medicine, Faculty of MedicineP.J. Safarik UniversityKosiceSlovakia

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