Curcumin Promotes Cell Cycle Arrest and Inhibits Survival of Human Renal Cancer Cells by Negative Modulation of the PI3K/AKT Signaling Pathway
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Curcumin possesses anti-cancer effects. In the current study, we tested the effect of curcumin on cell proliferation, viability, apoptosis, cell cycle phases, and activation of the PI3K/Akt pathway in the renal cell carcinoma (RCC) cell line RCC-949. We observed that cell proliferation and viability were markedly inhibited by curcumin, while cell apoptosis was promoted. The latter effect was associated with increased expression of Bcl-2 and diminished expression of Bax (both: mRNA and protein). The cells treated with curcumin increasingly went into cell cycle arrest, which was likely mediated by diminished expression of cyclin B1, as seen in curcumin-treated cells. In addition, curcumin decreased activation of the PI3K/AKT signaling pathway. In conclusion, our results demonstrate that curcumin exerts anti-cancer effects by negative modulation of the PI3K/AKT signaling pathway and may represent a promising new drug to treat RCC.
KeywordsHuman renal cell carcinoma Curcumin Cell apoptosis Cell cycle arrest AKT PI3K
- 2.Kuhara, H., Wakabayashi, T., Kishimoto, H., Sadoh, S., Suzuki, T., & Senda, Y. (1984). Malignant mediastinal myxoid tumor and renal cell carcinoma. Acta Patholoy Japan, 34, 881–887.Google Scholar
- 4.Haddad, A. Q., Wood, C. G., Abel, E. J., Krabbe, L. M., Darwish, O. M., Thompson, R. H., et al. (2014). Oncologic outcomes following surgical resection of renal cell carcinoma with inferior vena caval thrombus extending above the hepatic veins: A contemporary multicenter cohort. Journal of Urology, 192, 1050–1056.CrossRefPubMedGoogle Scholar
- 16.Uehara, Y., Inoue, M., & Fukuda, K. (2014). Inhibition of β-catenin and STAT3 with a curcumin analog suppresses gastric carcinogenesis in vivo. Gastric Cancer, 219, 950–957.Google Scholar
- 22.Liu, Z. L., & Mao, J. H. (2013). Inhibition of fatty acid synthase suppresses osteosarcoma cell invasion and migration via downregulation of the PI3K/Akt signaling pathway in vitro. Molecular Medicine Reports, 2013(7), 608–612.Google Scholar