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Cell Biochemistry and Biophysics

, Volume 73, Issue 2, pp 519–525 | Cite as

RETRACTED ARTICLE: VEGF Silencing Inhibits Human Osteosarcoma Angiogenesis and Promotes Cell Apoptosis via PI3K/AKT Signaling Pathway

  • Jian Zhao
  • Zi-Ru Zhang
  • Na Zhao
  • Bao-An Ma
  • Qing-Yu Fan
Original Paper

Abstract

Vascular endothelial growth factor (VEGF) is one of the most effective angiogenic factors that promote generation of tumor vasculature. VEGF is usually up-regulated in multiple cancers including osteosarcoma and glioma. To further explore the potential molecular mechanism that inhibits tumor growth induced by interference of VEGF expression, we constructed a Lv-shVEGF vector and assessed the efficiency of VEGF silencing and its influence in U2OS cells. The data demonstrate that Lv-shVEGF has high inhibition efficiency on VEGF expression, which inhibits proliferation and promotes apoptosis of U2OS cells in vitro. Our results also indicate that inhibition of VEGF expression suppresses osteosarcoma tumor growth in vivo and reduces osteosarcoma angiogenesis. We also found that the activations of phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT) were considerably reduced after osteosarcoma cells were treated with Lv-shVEGF. Taken together, our data demonstrate that VEGF silencing suppresses cell proliferation, promotes cell apoptosis, and reduces osteosarcoma angiogenesis through inactivation of PI3K/AKT signaling pathway.

Keywords

Cell apoptosis Angiogenesis Lentivirus-mediated short hairpin RNA (Lv-shRNA) U2OS cells Vascular endothelial growth factor (VEGF) 

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Copyright information

© Springer Science+Business Media New York 2015

Authors and Affiliations

  • Jian Zhao
    • 1
  • Zi-Ru Zhang
    • 1
  • Na Zhao
    • 2
  • Bao-An Ma
    • 1
  • Qing-Yu Fan
    • 1
  1. 1.Department of Orthopedic Surgery, Tangdu Hospital, Orthopedics Oncology Institute of Chinese PLAFourth Military Medical UniversityXi’anChina
  2. 2.Outpatient Department, Tangdu HospitalFourth Military Medical UniversityXi’anChina

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