Effects of TGF-β1 on the Proliferation and Apoptosis of Human Cervical Cancer Hela Cells In Vitro
- 427 Downloads
- 1 Citations
Abstract
To investigate the effects of TGF-β1 on the proliferation and apoptosis of cervical cancer Hela cells in vitro. Human cervical cancer Hela cells were cultured in vitro and divided into the experimental and control groups. In the experimental groups, Hela cells were stimulated with different concentrations of TGF-β1 (0.01, 0.1, 1, and 10 ng/mL), while Hela cells cultured in serum-free medium without TGF-β1 were used as controls. The CCK8 method was adopted to detect the effect of TGF-β1 on Hela cell proliferation, and flow cytometry was used to determine cell apoptosis 72 h after TGF-β1 treatment. Compared with the control group, the CCK-8 tests showed that different concentrations of TGF-β1 had no obvious effect on Hela cell proliferation 24 h after treatment (P > 0.05). However, upon 48 or 72 h of treatment, TGF-β1 significantly inhibited the proliferation of Hela cells in a time- and dose-dependent manner (P < 0.05). The flow cytometry results indicated that TGF-β1 influenced the apoptosis of human cervical cancer Hela cells in a dose-dependent manner after 72 h of treatment (P < 0.05). TGF-β1 significantly inhibited the growth and induced the apoptosis of human cervical Hela cells in vitro.
Keywords
TGF-β1 Cervical carcinoma Proliferation Apoptosis Cell cultureNotes
Acknowledgments
The research was funded by scientific research foundation item, the Department of Education, Sichuan Province, China (09ZA178).
Conflict of interest
The authors have not declared any conflicts of interest.
References
- 1.Smith, H. O., Tiffany, M. F., Qualls, C. R., et al. (2000). The rising incidence of adenocarcinoma relative to squamous cell carcinoma of the uterine cervix in the United States a 24-year population-based study. Gynecologic Oncology, 78(2), 97–105.CrossRefPubMedGoogle Scholar
- 2.Bommireddy, R., Saxena, V., Ormsby, et al. (2003). TGF-β1 lates lymphocyte homeostasis by preventing activation and subsequent apoptosis of peripheral lymphocytes. The Journal of Immunology, 170, 4612–4622.CrossRefPubMedGoogle Scholar
- 3.Bommireddy, R., Saxena, V., Ormsby, I., et al. (2003). TGF-β1 regulates lymphocyte homeostasis by preventing activation and subsequent apoptosis of peripheral lymphocytes. The Journal of Immunology, 170(9), 4612–4622.CrossRefPubMedGoogle Scholar
- 4.Chen, Kai, Sun, Huiyan, Mou, Xinhong, et al. (2012). Tumour cell apoptosis mediated by the TGF-β/Smad and TGF-β/MAPK pathways. Acta Academiae Medicinae CPAF, 21(2), 144–146.Google Scholar
- 5.Qin, Dongchun, Wang, Haiying, & Zhang, Qinxian. (2007). Effect of the transforming growth factor β1 on the proliferation of hepatocellular carcinoma cell line HepG-2. Journal of Medical Form, 28(02), 8–10.Google Scholar
- 6.Zhong, Liang, Chen, Jian, Jin, Xu, et al. (2008). Effect of TGF-β1 on the proliferation of pancreatic carcinoma BxPC-3 cells and the primary mechanisms. Fudan University Journal of Medical Sciences, 35(2), 181–185.Google Scholar
- 7.Melet, A., Song, K., Bucur, O., et al. (2007). Apoptotic pathways in tumor progression and therapy. Programmed cell death in cancer progression and therapy (pp. 47–79). Netherlands: Springer.Google Scholar
- 8.Wang, C. L., Wan, Y. L., Liu, Y. C., & Huang, Z. Q. (2006). Apoptosis of human hepatoma cell lines induced by transforming growth factor beta 1(TGF-ββ1)correlates with p53 and Smad4 activation. Journal of Peking University (Healthy Sciences), 38(02), 176–178.Google Scholar
- 9.Wang, Xiang, Zhang, Runqi, Yan, Aili, et al. (2011). TGF-ββ1 induces the apoptosis of gastric cancer BGC823 cells through Bim protein. Journal of Modern Oncology, 18(10), 1899–1901.Google Scholar
- 10.Ansieau, S., Bastid, J., Doreau, A., et al. (2008). Induction of EMT by twist proteins as a collateral effect of tumor-promoting inactivation of premature senescence. Cancer Cell, 14(1), 79–89.CrossRefPubMedGoogle Scholar