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Cell Biochemistry and Biophysics

, Volume 70, Issue 1, pp 677–686 | Cite as

Simultaneous Determination of Calycosin-7-O-β-d-Glucoside, Ononin, Calycosin, Formononetin, Astragaloside IV, and Astragaloside II in Rat Plasma After Oral Administration of Radix Astragali Extraction for Their Pharmacokinetic Studies by Ultra-Pressure Liquid Chromatography with Tandem Mass Spectrometry

  • Xiao-hua Liu
  • Jian-bang Zhao
  • Long Guo
  • Ying-lai Yang
  • Fang Hu
  • Rui-juan Zhu
  • Shi-lan Feng
Original Paper

Abstract

A sensitive and reliable ultra-pressure liquid chromatography with tandem mass spectrometry (UPLC–MS) was developed and validated for simultaneous quantification of six main bioactive components, i.e., calycosin-7-O-β-d-glucoside, ononin, calycosin, formononetin, astragaloside IV, and astragaloside II in rat plasma after oral administration of the 95 % ethanol extraction from Radix Astragali. Plasma samples were extracted with Waters OasisTM HLB 1 cc (30 mg) Extraction Cartridges (SPE) separated on an UPLC™ BEH C18 column and detected by MS with electro spray ionization interface in positive selective ion monitoring mode. Calibration curves offered linear ranges of two orders of magnitude with r 2 > 0.99. The method had the lower limit quantification of 1.30, 0.73, 1.17, 2.33, 0.63, and 0.83 ng/mL for ononin, calycosin, calycosin-7-O-β-d-glucoside, formononetin, astragaloside IV, and astragaloside II, respectively, with precision less than 10 %. The RSD of intra- and inter-day variations ranged from 1.66 to 6.46 and 3.39 to 6.58 %. This developed method was applied subsequently to pharmacokinetic studies of the six compounds in rats successfully. The proposed method was for the first time to compare the pharmacokinetic difference between calycosin-7-O-β-d-glucoside and calycosin in rat plasma, so as between ononin and formononetin, and studied to the astragaloside II pharmacokinetics in rat plasma.

Keywords

UPLC–MS Calycosin-7-O-β-d-glucoside Ononin Calycosin Formononetin Astragaloside IV Astragaloside II Radix Astragali extraction Pharmacokinetic 

Notes

Acknowledgments

The authors greatly appreciate financial support from the great research projects (lzujbky-2013-198) and (No. z2012117) of the Ministry of Education of China, (No. 2011BAI05B00) of the Ministry of Science and Technology of China. The authors would like to thank Professor Rong Wang, for providing instrumental facilities of LC–MS from Department of Pharmacy, Lanzhou General Hospital of PLA.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Xiao-hua Liu
    • 1
    • 2
  • Jian-bang Zhao
    • 3
  • Long Guo
    • 1
  • Ying-lai Yang
    • 1
  • Fang Hu
    • 1
  • Rui-juan Zhu
    • 1
  • Shi-lan Feng
    • 1
  1. 1.School of PharmacyLanzhou UniversityLanzhouPeople’s Republic of China
  2. 2.College of Chemistry and Chemical EngineeringLanzhou UniversityLanzhouPeople’s Republic of China
  3. 3.Food and Drug Control of GansuLanzhouPeople’s Republic of China

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