Cell Biochemistry and Biophysics

, Volume 70, Issue 1, pp 643–649 | Cite as

Effects of 900 MHz Radiofrequency Radiation on Skin Hydroxyproline Contents

  • Semra Tepe Çam
  • Nesrin Seyhan
  • Cengiz Kavaklı
  • Ömür Çelikbıçak
Original Paper


The present study aimed to investigate the possible effect of pulse-modulated radiofrequency radiation (RFR) on rat skin hydroxyproline content, since skin is the first target of external electromagnetic fields. Skin hydroxyproline content was measured using liquid chromatography mass spectrometer method. Two months old male wistar rats were exposed to a 900 MHz pulse-modulated RFR at an average whole body specific absorption rate (SAR) of 1.35 W/kg for 20 min/day for 3 weeks. The radiofrequency (RF) signals were pulse modulated by rectangular pulses with a repetition frequency of 217 Hz and a duty cycle of 1:8 (pulse width 0.576 ms). A skin biopsy was taken at the upper part of the abdominal costa after the exposure. The data indicated that whole body exposure to a pulse-modulated RF radiation that is similar to that emitted by the global system for mobile communications (GSM) mobile phones caused a statistically significant increase in the skin hydroxyproline level (p = 0.049, Mann–Whitney U test). Under our experimental conditions, at a SAR less than the International Commission on Non-Ionizing Radiation Protection safety limit recommendation, there was evidence that GSM signals could alter hydroxyproline concentration in the rat skin.


Radiofrequency radiation 900 MHz Skin Hydroxyproline Collagen LC MS/MS 



Radiofrequency radiation


Liquid chromatography mass spectrometer


Specific absorption rate




Global system for mobile communications


International commission on non-ionizing radiation protection


Electromagnetic radiation


Extremely low frequency


Atmospheric pressure chemical ionization



We are indebted to Dr. Henry Lai (Department of Bioengineering, University of Washington) for reviewing the manuscript.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Semra Tepe Çam
    • 1
  • Nesrin Seyhan
    • 1
  • Cengiz Kavaklı
    • 2
  • Ömür Çelikbıçak
    • 2
  1. 1. Biophysics Department, Faculty of MedicineGazi UniversityAnkaraTurkey
  2. 2.Chemistry DepartmentHacettepe UniversityAnkaraTurkey

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