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Cell Biochemistry and Biophysics

, Volume 70, Issue 1, pp 333–336 | Cite as

The Attenuation of Lung Ischemia Reperfusion Injury by Oxymatrine

  • Bing ZhuEmail author
  • Jian-Ru Yang
  • Shi-Feng Chen
  • Yue Quan Jiang
Original Paper

Abstract

To investigate the protective effects of oxymatrine (OMT) on lung ischemia reperfusion injury (LIRI) in rabbits, models of LIRI in rabbit were used. Thirty-two rabbits were randomly divided into four groups: control group (n = 8), ischemia reperfusion group (I/R group, n = 8), OMTl group (n = 8), OMT2 group (n = 8). Lung tissue samples were collected at 40, 80, 120 min time-points after lung ischemia reperfusion. TNF-α, 1I-8, IL-10, apoptosis index (AI), and index of quantitative assessment of histologic lung injury (IQA) were measured in each group. TNF-α and IL-8 in I/R group were significantly higher than those of the control group and OMT2 group (P < 0.01), but in OMT2 group they were significantly lower than those of OMTl group (P < 0.05). IL-10 in OMT2 group and OMTl group was significantly higher than that of I/R group (P < 0.01). But in OMTl group it was significantly lower than that of OMT2 group (P < 0.05). AI in I/R group was significantly higher than that of OMT2 group and the control group at 80 min after lung ischemia reperfusion (P < 0.01). IQA in OMTl group and OMT2 group was significantly lower than that of the I/R group (P < 0.01). Oxymatrine can protect against LIRI in rabbits by upregulating levels of IL-10 and downregulating levels of TNF-α and IL-8, inhibiting the alveolar cells apoptosis and inflammatory response, and attenuating the acute LIRI.

Keywords

Lung ischemia reperfusion injury Oxymatrine TNF-α IL-8 IL-10 

Notes

Acknowledgments

This study was supported by the National Natural Sciences Foundation of China (Grant No. 30471984), the Foundation of Bureau of Public Health of Chongqing (Grants 06-2-001, 06-B-26 and 2010-2-127), and the project Innovation of Science and Technology, Chongqing Science and Technology Commission (Project No. cstc2013jcyjA10108).

References

  1. 1.
    Murata, T., Nakazawa, H., Mori, I., et al. (1992). Reperfusion after a two hour period of pulmonary artery occlusion cause pulmonary necrosis. American Review of Respiratory Disease, 146(4), 1048.PubMedCrossRefGoogle Scholar
  2. 2.
    Ymashita, H., Akamine, S., Sumida, Y., et al. (2004). Inhaled nitric oxide attenuates apoptosis in ischemia-reperfusion injury of the rabbit lung. Annals of Thoracic Surgery, 78(1), 292.CrossRefGoogle Scholar
  3. 3.
    Goodman, R. B., Pugin, J., Lee, J. S., et al. (2003). Cytokine-mediated inflammation in acute lung injury. Cytokine & Growth Factor Reviews, 14(6), 523.CrossRefGoogle Scholar
  4. 4.
    Jimenez, L. A., Drost, E. M., Gilmour, P. S., et al. (2002). PMl0-exposed macrophages stimulate a proinflammatory response in lung epithelial cells via TNF-α. American Journal of Physiology. Lung Cellular and Molecular Physiology, 282(2), L237.PubMedGoogle Scholar
  5. 5.
    Xiang, M.-Z., Jiang, Y.-G., & Wang, R.-W. (1999). The change of TNF-α, IL-6, IL-8 in lung and plasma in rats with lung ischemia reperfusion injury and their significance. Chongqing Yixue, 28(1), 5.Google Scholar
  6. 6.
    Albrecht, C., Schins, R. P., Hohr, D., et al. (2004). Inflammatory time course after quartz instillation: Role of tumor necrosis factor-α and particle surface. American Journal of Respiratory Cell and Molecular Biology, 31(3), 292.PubMedCrossRefGoogle Scholar
  7. 7.
    Li, Y.-S., Xu, Y.-C., Tu, Z.-C., et al. (2005). Chemokine gene expression in pancreatic tissue and effects of oxymatrine in early acute necrotizing pancreatitis. World Chinese Journal of Digestology, 13(8), 979.Google Scholar
  8. 8.
    Zhuang, J.-W., Fang, D., & Zhang, H.-G. (2008). Expression of TNF-α mRNA and IL-1β mRNA in rats of oxymatrine treatment for acute hemorrhagic necrotizing pancreatitis. Journal of Modern Laboratory Medicine, 23(4), 59.Google Scholar
  9. 9.
    Shanley, T. P., Vasi, N., & Denenbery, A. (2000). Regulation of chemokine expression by IL-IO in lung inflammation. Cytokine, 12(7), 1054.PubMedCrossRefGoogle Scholar
  10. 10.
    Zhou, Z.-X., Jia, X.-M., Huang, J.-J., et al. (2006). Changes and significance of mitogen-activated protein kinase (MAPKS) during lung ischemia-reperfusion injury. Chongqing Yixue, 35(3), 1189.Google Scholar
  11. 11.
    Xu, G. L., Yao, L., Gong, S. Q., et al. (2005). Attenuation of acute lung injury in mice by oxymatrine is associated with inhibition of phosphorylated p38 mitogen-activated protein kinese. Journal of Ethnopharmacology, 98(1\2), 177.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Bing Zhu
    • 1
    Email author
  • Jian-Ru Yang
    • 2
  • Shi-Feng Chen
    • 1
  • Yue Quan Jiang
    • 1
  1. 1.Department of Cardiothoracic Surgery, The Second Affiliated HospitalChongqing Medical UniversityChongqingChina
  2. 2.Central LaboratoryNo. 6 Hospital of HandanHandanChina

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