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Cell Biochemistry and Biophysics

, Volume 69, Issue 3, pp 439–444 | Cite as

Correlation of Matrix Metalloproteinases-1 and Tissue Inhibitor of Metalloproteinases-1 with Patient Age and Grade of Lumbar Disk Herniation

  • Haidong Xu
  • Qiang Mei
  • Jin He
  • Gang Liu
  • Jianning Zhao
  • Bin XuEmail author
Original Paper

Abstract

The authors studied the nuclear magnetic resonance films and the expression of MMP-1 and TIMP-1 in disk specimens’ of patients who had undergone operations for lumbar disk herniation. Forty-one lumbar disk patients were evaluated imaging for degenerative changes and their disk specimens immunohistochemical expression of MMP-1 and TIMP-1. The degree of degenerative changes was based on magnetic resonance imaging films. Sections of disk immunostained for MMP-1 and TIMP-1 were evaluated semiquantitatively. Patients were categorized in three age groups: <30 years, from 30 to 60 years, and >60 years of age. The expressions of MMP-1 and TIMP-1 were related to patients’ age and degree of degenerative changes. There were statistical differences in the expression of MMP-1 and TIMP-1 between the age and degree of degenerative changes groups. With the degree of degenerative changes, the expression of MMP-1 and TIMP-1 increased obviously. But in old age group, the expression of MMP-1/TIMP-1 was higher than the young groups. The expressions of MMP-1 and TIMP-1 were strongly correlated to the age and the degree of the degenerative changes. An important finding in this study is the unbalance of the expression of MMP-1 and TIMP-1 along with the growth of the age.

Keywords

MMP-1 TIMP-1 Lumbar spine herniation Immunohistochemistry 

Abbreviations

MMP-1

Matrix metalloproteinases-1

TIMP-1

Tissue inhibitor of metalloproteinases-1

DAB

Diaminobenzidine tetrahydrochloride

Notes

Conflict of interest

The authors have no conflict of interest to declare.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Haidong Xu
    • 1
  • Qiang Mei
    • 2
  • Jin He
    • 3
  • Gang Liu
    • 1
  • Jianning Zhao
    • 1
  • Bin Xu
    • 1
    Email author
  1. 1.Department of Orthopedics of Jinling HospitalNanjing University, School of MedicineNanjingChina
  2. 2.Department of Gastroenterology of the 169th Hospital, School of MedicineHunan Normal UniversityChangshaChina
  3. 3.Department of Neurosurgery of Jinling HospitalNanjing University, School of MedicineNanjingChina

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