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Cell Biochemistry and Biophysics

, Volume 71, Issue 3, pp 1671–1676 | Cite as

Effect of Mechanism of Action of Different ω-6/ω-3 Polyunsaturated Fatty Acids Ratio on the Growth of Endometrial Carcinoma Mice

  • Xiaoyuan LuEmail author
  • Xuan Ding
  • Li Jing
Original Paper

Abstract

To explore the effect and mechanism of action of different ω-6/ω-3 polyunsaturated fatty acids (PUFAs) ratio on the expression of AKT and mTOR in mice bearing endometrial carcinoma. Once the human endometrial carcinoma xenograft models were successfully established, 40 BALB/C mice were randomized into five groups: group A (ω-6 PUFAs), group B (10:1 ω-6/ω-3 PUFAs), group C (control group), group D (1:1 ω-6/ω-3 PUFAs), and group E (ω-3 PUFAs). Six weeks post-treatment, mice were sacrificed and the xenograft tissues were harvested for immunohistochemical SP analysis of AKT and mTOR expression. AKT and mTOR mRNA expression was determined by reverse transcription polymerase chain reaction. Group A and group B had the highest positive expression of AKT and mTOR, with increased mRNA expression. Group D and group E had the lowest positive expression of AKT and mTOR, with decreased mRNA expression. There was a positive correlation between the expression of AKT and that of mTOR (r = 0.92). Thus, ω-6/ω-3 PUFAs in different proportions are associated with the mRNA expression of AKT and mTOR in the tissues of mouse xenograft model of human endometrial cancer.

Keywords

Polyunsaturated fatty acids Endometrial carcinoma mTOR AKT 

Notes

Acknowledgments

We would like to thank Science and technology Bureau of Xuzhou, Jiangsu province, China to support Science and Technology Projects of Xuzhou 2013 (No. XM13B099).

Conflict of interest

Authors declare no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Gynaecology and ObstetricsAffiliated Hospital of Xuzhou Medical CollegeXuzhouChina

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