Expression of Sclerostin scFv and the Effect of Sclerostin scFv on Healing of Osteoporotic Femur Fracture in Rats
- 332 Downloads
Osteoporosis is a systemic metabolic disease characterized by low bone mass with deterioration of the bony microstructure which leads to both bone brittleness and increased risk of fracture. Sclerostin is a protein encoded by the SOST gene which is specifically expressed in osteocyte. Monoclonal antibodies of sclerostin can promote bone formation by antagonizing its inhibitory action. However, the effectiveness of monoclonal antibodies to exert such effects are limited by the large molecular mass and high immunogenicity. Here, we report that we purified a high immune affinity, single-chain antibody of SOST: SOST–single-chain Fv (scFv). Real-time polymerase chain reaction amplification of the variable regions of the heavy- and light-chain gene from a secretory anti-SOST antibody was performed. Animal experiments showed that SOST–scFv promoted bone healing in a rat model of osteoporosis.
KeywordsOsteoporosis Bone healing SOST SOST–scFv scFv Sclerostin Femur fracture VH and VL gene
The work is supported by the National Natural Science Foundation of China (under Grant 81000796, 81141012) and Beijing Nova Program (under Grant 2011085, 2011120).
- 11.Ahmad, Z. A., et al. (2012). scFv antibody: Principles and clinical application. Clinical and Developmental Immunology. Article ID 980250. doi: 10.1155/2012/980250.
- 13.Kong, H. Y., Chen, Y. P., Yang, S. Q., et al. (2004). Experimental analysis of osteoporotic detection marker of ovariectomized rats. Chinese Journal of Orthopaedic Trauma, 17(4), 204–206.Google Scholar