Cell Biochemistry and Biophysics

, Volume 69, Issue 1, pp 47–54 | Cite as

The Time-Dependent Manner of Sinusoidal Electromagnetic Fields on Rat Bone Marrow Mesenchymal Stem Cells Proliferation, Differentiation, and Mineralization

  • Ming-Yu Song
  • Ji-Zhe Yu
  • Dong-Ming Zhao
  • Sheng Wei
  • Yang Liu
  • Yue-Ming Hu
  • Wen-Chun Zhao
  • Yong Yang
  • Hua Wu
Original Paper


Electromagnetic fields (EMFs) are used clinically to promote fracture healing and slow down osteoporosis without knowledge of optimal parameters and underlying principles. In the present study, we investigate the effects of irritation for different durations with 15 Hz 1 mT sinusoidal EMFs (SEMFs) on rat bone marrow mesenchymal stem cells (BMSCs) proliferation, differentiation, and mineralization potentials. Our results show that SEMFs irritation promote rat BMSCs proliferation in a time-dependent manner, and the expression of osteogenic gen [Cbfa 1/RUNX2, bone sialoprotein (BSP), osteopontin (OPN)], alkaline phosphatase activity, and calcium deposition were enhanced after SEMFs treatment depending on the time duration of treatment. To determine the role of MEK/ERK signaling pathway, U0126, a MEK/ERK inhibitor was used. It can suppress rat BMSCs’ proliferation with or without SEMF exposure, and partly attenuate the expression of osteogenesis related proteins (RUNX2, BSP, OPN) which were improved by SEMF. This finding suggests that the effects of SEMF on rat BMSCs’ proliferation differentiation and mineralization are time duration dependent and MEK/ERK signaling pathway plays important role.


Sinusoidal electromagnetic fields Bone marrow mesenchymal stem cells Differentiation Proliferation 



The study was supported by National Natural Science Foundation of China (Grant No. 51077065).

Conflict of interest

The authors declare no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Ming-Yu Song
    • 1
  • Ji-Zhe Yu
    • 1
  • Dong-Ming Zhao
    • 1
  • Sheng Wei
    • 1
  • Yang Liu
    • 1
  • Yue-Ming Hu
    • 1
  • Wen-Chun Zhao
    • 2
  • Yong Yang
    • 1
  • Hua Wu
    • 1
  1. 1.Department of Orthopedics, Tongji Hospital, Tongji Medical CollegeHuazhong University of Science and TechnologyWuhanChina
  2. 2.Navy University of EngineeringWuhanChina

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