Cell Biochemistry and Biophysics

, Volume 66, Issue 1, pp 187–198 | Cite as

Differential Effects of G- and F-Actin on the Plasma Membrane Calcium Pump Activity

  • Laura Vanagas
  • María Candelaria de La Fuente
  • Marianela Dalghi
  • Mariela Ferreira-Gomes
  • Rolando C. Rossi
  • Emanuel E. Strehler
  • Irene C. Mangialavori
  • Juan P. F. C. Rossi
Original Paper


We have previously shown that plasma membrane calcium ATPase (PMCA) pump activity is affected by the membrane protein concentration (Vanagas et al., Biochim Biophys Acta 1768:1641–1644, 2007). The results of this study provided evidence for the involvement of the actin cytoskeleton. In this study, we explored the relationship between the polymerization state of actin and its effects on purified PMCA activity. Our results show that PMCA associates with the actin cytoskeleton and this interaction causes a modulation of the catalytic activity involving the phosphorylated intermediate of the pump. The state of actin polymerization determines whether it acts as an activator or an inhibitor of the pump: G-actin and/or short oligomers activate the pump, while F-actin inhibits it. The effects of actin on PMCA are the consequence of direct interaction as demonstrated by immunoblotting and cosedimentation experiments. Taken together, these findings suggest that interactions with actin play a dynamic role in the regulation of PMCA-mediated Ca2+ extrusion through the membrane. Our results provide further evidence of the activation–inhibition phenomenon as a property of many cytoskeleton-associated membrane proteins where the cytoskeleton is no longer restricted to a mechanical function but is dynamically involved in modulating the activity of integral proteins with which it interacts.


PMCA Cytoskeleton Actin Regulation 



Dimethyl sulfoxide


Ethylene glycol tetraacetic acid


Inside-out vesicles


2-Amino-6-mercapto-7-methylpurine riboside


Plasma membrane calcium ATPase


Tris(hydroxymethyl) aminomethane


Polyoxyethylene glycol monoether with C12 alkyl chain and 10 polyoxyethylene units in the headgroup



The present work was supported by the NIH, Fogarty International Center Grant R03TW006837 to JPFCR and by ANPCYT, CONICET and UBACYT from Argentina. MCDLF, LV, MD, and MFG are doctoral fellows of CONICET. ICM, RCR and JPFCR are established investigators of CONICET, Argentina. EES is an established researcher of Mayo/Clinic Foundation, Rochester, MN, USA.


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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Laura Vanagas
    • 1
  • María Candelaria de La Fuente
    • 1
  • Marianela Dalghi
    • 1
  • Mariela Ferreira-Gomes
    • 1
  • Rolando C. Rossi
    • 1
  • Emanuel E. Strehler
    • 2
  • Irene C. Mangialavori
    • 1
  • Juan P. F. C. Rossi
    • 1
  1. 1.IQUIFIB, Facultad de Farmacia y BioquímicaUniversidad de Buenos AiresBuenos AiresArgentina
  2. 2.Department of Biochemistry and Molecular BiologyMayo Clinic College of MedicineRochesterUSA

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