Cell Biochemistry and Biophysics

, Volume 66, Issue 2, pp 337–346 | Cite as

Neuroprotective Effect of Atorvastatin Involves Suppression of TNF-α and Upregulation of IL-10 in a Rat Model of Intracerebral Hemorrhage

  • Tu Ewen
  • Liu Qiuting
  • Tang Chaogang
  • Tang Tao
  • Wu Jun
  • Tan Liming
  • Xiang Guanghong
Translational Biomedical Research

Abstract

We evaluated the neuroprotective effects of atorvastatin (2, 5, and 10 mg/kg) on experimentally induced intracerebral hemorrhage (ICH) in adult rats; controls were administered PBS. Plasma TNF-α and IL-10 levels before and after ICH were analyzed at various time points by enzyme-linked immunosorbent assay (ELISA) and neurological behavior of rats was assessed by climbing scores. At 3-days postoperatively, brain water contents and TNF-α/IL-10 expression in brain tissue were determined. Histopathological changes and microglial cells in the brain tissue were evaluated by light-microscopy. Post-ICH neurological deficits differed significantly between sham-operated group A and experimental-ICH group B (P < 0.05). Brain water contents were significantly less in group A than in group B (P < 0.05). Significant differences (P < 0.05) between two groups were observed regarding activated microglia, TNF-α and IL-10 levels. Compared with group B, neurological deficits, brain water contents, pathological changes, and activated microglia were reduced (P < 0.05) in groups C (Experimental-ICH + atorvastatin 2 mg/kg), D (Experimental-ICH + atorvastatin 5 mg/kg) and E (Experimental-ICH + atorvastatin 10 mg/kg). Atorvastatin-induced a dose-dependent reduction of TNF-α and increase of IL-10 levels (P < 0.05). Therefore, it was concluded that atorvastatin improved neurofunctional rehabilitation in rats through the suppression of cytokines-mediated inflammatory response and attenuation of brain damage following intracerebral hemorrhage.

Keywords

Experimental-intracerebral hemorrhage Atorvastatin Acute inflammatory reaction TNF-α IL-10 

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Tu Ewen
    • 1
  • Liu Qiuting
    • 1
  • Tang Chaogang
    • 1
  • Tang Tao
    • 1
  • Wu Jun
    • 1
  • Tan Liming
    • 1
  • Xiang Guanghong
    • 1
  1. 1.Department of NeurologyThe Brains Hospital of Hunan ProvinceChangshaChina

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