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Cell Biochemistry and Biophysics

, Volume 63, Issue 3, pp 247–259 | Cite as

Effect of Sulforaphane on Growth Inhibition in Human Brain Malignant Glioma GBM 8401 Cells by Means of Mitochondrial- and MEK/ERK-Mediated Apoptosis Pathway

  • Tzuu-Yuan HuangEmail author
  • Weng-Cheng Chang
  • Miin-Yau Wang
  • Yun-Ru Yang
  • Yi-Chiang HsuEmail author
Original Paper

Abstract

In recent studies, sulforaphane (SFN) has been seen to demonstrate antioxidant and anti-tumor activities as well as potent chemopreventive action against cancer. The present study investigates the anti-proliferation (using MTT assay, SFN demonstrated cytotoxic activity against GBM 8401 cell with IC50 values at 35.52 μM) and induced apoptosis of SFN 24-h treatment in the cells of human brain malignant glioma GBM 8401 cells. We studied the MMP, caspase, MEK/ERK activation, and NF-κB transcription factor activity. Our results indicate that SFN inhibits cell proliferation as well as the activation of apoptosis in GBM 8401 cells. Both effects increased in proportion to the dosage of SFN, and apoptosis was induced via mitochondria- and caspase-dependent pathways. Daily s.c. injections of SFN for 3 weeks in severe combined immunodeficient mice (SCID) with GBM8401 s.c. tumors resulted in a decrease in mean tumor weight of 69–75 % compared with vehicle-treated controls. Our findings suggest that, in addition to the known effects on cancer prevention, SFN may provide antitumor activity in established malignant glioma.

Keywords

Sulforaphane Malignant glioma Mitochondrial MEK/ERK pathway Apoptosis 

Notes

Acknowledgments

The authors gratefully acknowledge the financial support from Tainan Sin-Lau Hospital (99-01 and 100-01).

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Copyright information

© Springer Science+Business Media, LLC 2012

Authors and Affiliations

  1. 1.Department of NeurosurgeryTainan Sin-Lau HospitalTainanTaiwan
  2. 2.Graduate Institute of Medical Science, College of Health SciencesChang Jung Christian UniversityTainanTaiwan
  3. 3.Innovative Research Center of Medicine, College of Health SciencesChang Jung Christian UniversityTainanTaiwan

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