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Cell Biochemistry and Biophysics

, Volume 62, Issue 2, pp 281–287 | Cite as

Inflammatory Lesions of Cochlea in Murine Cytomegalovirus-Infected Mice with Hearing Loss

  • Qiao Yuehua
  • Zhang Longzhen
  • Xu KailinEmail author
  • Zeng Lingyu
  • Meng Lingjian
  • Wang Jun
  • Meng Hong
Original Paper

Abstract

We sought to determine the distribution of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and lymphocytes in the cochlea of mice infected with murine cytomegalovirus (MCMV). For this purpose, 16 newborn mice were divided equally into model and control (uninfected) groups. In model group, 10 μl of MCMV was injected into the brain of each mouse whereas in control group, 10 μl of physiological saline was injected. Fourteen days after the injection of MCMV, the auditory brainstem response audiometry was performed. Later, the mice were killed and the acoustic capsule samples were collected for polymerase chain reaction analysis, histopathological and immunohistochemical studies. Compared with control mice, the incubation period was longer (F = 13.797; P = 0.003) and the amplitude was lower (F = 5.095; P = 0.043) in model group mice. It indicated that the intracerebral injection of MCMV caused hearing loss in mice. Histopathological examination of cochleas revealed increased levels of lymphocytic infiltration in the membrana vestibularis. Higher levels of TNF-α and IL-6 in scala tympani were detected by immunohistochemical staining. Taken together, the hearing loss in mice could be related with the inflammatory changes occurring in cochlea after inception of MCMV infection.

Keywords

Murine cytomegalovirus Cochlear inflammation Hearing loss Tumor necrosis factor (TNF)-α Interleukin (IL)-6 

Notes

Acknowledgments

We thank the Xuzhou Technology Development Fund, China for financial support (Grant # XM07C071). We are also indebted to the Microbiological Laboratory, Basic Medicine Institute, Shandong Province Academy of Medical Sciences, China for kindly providing 3T3 cells and MCMV Smith strain.

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Qiao Yuehua
    • 1
  • Zhang Longzhen
    • 2
  • Xu Kailin
    • 3
    Email author
  • Zeng Lingyu
    • 3
  • Meng Lingjian
    • 4
  • Wang Jun
    • 4
  • Meng Hong
    • 5
  1. 1.Department of OtolaryngologyXuzhou Medical CollegeXuzhouChina
  2. 2.Cancer Research CenterXuzhou Medical CollegeXuzhouChina
  3. 3.Transplant Immune LaboratoryXuzhou Medical CollegeXuzhouChina
  4. 4.Department of PaediatricsAffiliated Hospital of Xuzhou Medical CollegeXuzhouChina
  5. 5.Laboratory of Microbiology, Basic Medicine InstituteShandong Province Academy of Medical SciencesJinanChina

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