Inflammatory Lesions of Cochlea in Murine Cytomegalovirus-Infected Mice with Hearing Loss
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We sought to determine the distribution of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and lymphocytes in the cochlea of mice infected with murine cytomegalovirus (MCMV). For this purpose, 16 newborn mice were divided equally into model and control (uninfected) groups. In model group, 10 μl of MCMV was injected into the brain of each mouse whereas in control group, 10 μl of physiological saline was injected. Fourteen days after the injection of MCMV, the auditory brainstem response audiometry was performed. Later, the mice were killed and the acoustic capsule samples were collected for polymerase chain reaction analysis, histopathological and immunohistochemical studies. Compared with control mice, the incubation period was longer (F = 13.797; P = 0.003) and the amplitude was lower (F = 5.095; P = 0.043) in model group mice. It indicated that the intracerebral injection of MCMV caused hearing loss in mice. Histopathological examination of cochleas revealed increased levels of lymphocytic infiltration in the membrana vestibularis. Higher levels of TNF-α and IL-6 in scala tympani were detected by immunohistochemical staining. Taken together, the hearing loss in mice could be related with the inflammatory changes occurring in cochlea after inception of MCMV infection.
KeywordsMurine cytomegalovirus Cochlear inflammation Hearing loss Tumor necrosis factor (TNF)-α Interleukin (IL)-6
We thank the Xuzhou Technology Development Fund, China for financial support (Grant # XM07C071). We are also indebted to the Microbiological Laboratory, Basic Medicine Institute, Shandong Province Academy of Medical Sciences, China for kindly providing 3T3 cells and MCMV Smith strain.
- 1.Biheng, C., Liangzhen, W., Ying, Z., Dan, L., & Nan, W. (2007). Expression of high mobility group box chromosomal protein 1 in inner ear of mice infected by murine cytomegalovirus. The Journal of Practical Medicine, 23, 336–338.Google Scholar
- 2.Liangzhen, W. (2005). Human cytomegalovirus intrauterine infection and prognosis, a review. Chinese Journal of Practical Gynecology and Obstetrics, 21, 331–332.Google Scholar
- 5.Robe, F. P. (2005). Congenital cytomegalovirus infection and hearing Loss. Herpes, 12, 50–56.Google Scholar
- 9.Wei, M., Wei, Z., Yiying, Z., et al. (2005). Hearing screening in infants with congenital cytomegalovirus infection. Journal of Zhejiang University (Medical Sciences), 34, 358–360.Google Scholar
- 12.Jiangying, S., Min, G., Zhaichuan, Z., Huankui, L., & Youzhen, W. (2004). The improvement and application of inner ear tissue parafin section technique. Chinese Journal of Diagnostic Pathology, 10, 359.Google Scholar
- 13.Zhijian, C., Xingsan, T., Xiang, G., Yuhua, M., Kun, L., & Jihua, D. (2005). Expression of IL-1β and TNF-α in the myocardium of MCMV myocarditis and its significance. Shandong Medical Journal, 45, 3–5.Google Scholar
- 15.Yan, F., Suhua, C., Li, C., Juanjuan, C., Zhijun, Z., & Xiazhen, L. (2007). The research of murine inborn hearing change after microinjecting cytomegalovirus via the placenta way. Chinese Journal of Birth Health and Heredity, 15, 27–29.Google Scholar