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Cell Biochemistry and Biophysics

, Volume 61, Issue 2, pp 337–348 | Cite as

Inhibitory Effect of Delphinidin on Monocyte–Endothelial Cell Adhesion Induced by Oxidized Low-Density Lipoprotein via ROS/p38MAPK/NF-κB Pathway

  • Chun-ye Chen
  • Long Yi
  • Xin Jin
  • Ting Zhang
  • Yu-jie Fu
  • Jun-dong Zhu
  • Man-tian Mi
  • Qian-yong Zhang
  • Wen-hua Ling
  • Bin Yu
Original Paper

Abstract

Monocyte adhesion to the vascular endothelium and their subsequent trans-endothelial migration are pivotal early events in atherogenesis. In this study, the effect of delphinidin, belonging to the group of anthocyanin, on adhesion of monocytes to endothelial cells induced by ox-LDL was investigated. The results showed that the pre-treatment with delphinidin (50, 100, or 200 μM) dose-dependently decreased the ox-LDL-induced up-regulation of the expression of ICAM-1 and P-selectin, and the enhanced adhesion and transmigration of monocytes. To determine the role of ROS/p38MAPK/NF-κB pathway, intracellular ROS level, p38MAPK protein expression, NF-κB transcription activity and protein expression, IκB-α degradation, NADPH oxidase subunit (Nox2 and p22phox) protein, and mRNA expression were measured. The results showed that delphinidin attenuated ox-LDL-induced generation of ROS, p38MAPK protein expression, NF-κB transcription activity and protein expression, IκB-α degradation, NADPH oxidase subunit (Nox2 and p22phox) protein and mRNA expression in endothelial cells in a dose-dependent manner. These results suggest that delphinidin attenuates ox-LDL induced expression of adhesion molecules (P-selectin and ICAM-1) and the adhesion of monocytes to endothelial cells by inhibiting ROS/p38MAPK/NF-κB pathway. These findings provide a basis for the design of potent antiatherosclerotic agents that will have therapeutic potential in the prevention of AS.

Keywords

Delphinidin Monocyte Endothelial cell Adhesion Reactive oxygen species Nuclear factor-κB 

Abbreviations

ox-LDL

Oxidized low density lipoprotein

NADPH

Nicotinamide adenine dinucleotide phosphate

ROS

Reactive oxygen species

NF-κB

Nuclear factor-κB

MCP-1

Monocyte chemoattractant protein-1

LOX-1

Lectin-like low density lipoprotein-1

ICAM-1

Intercellular adhesion molecule-1

VCAM-1

Vascular cell adhesion molecule-1

sICAM-1

Soluble intercellular adhesion molecule-1

AS

Atherosclerosis

Notes

Acknowledgments

This work was supported by National Natural Science Foundation of China (grant number 81000133), “the 11th Five-year Plan” for National Key Technology Research and Development Program (2008BAI58B06), and the Innovation Project of Chongqing Key Laboratory of Nutrition and Food Safety (2006CA1003).

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Chun-ye Chen
    • 1
  • Long Yi
    • 1
  • Xin Jin
    • 1
  • Ting Zhang
    • 1
  • Yu-jie Fu
    • 1
  • Jun-dong Zhu
    • 1
  • Man-tian Mi
    • 1
  • Qian-yong Zhang
    • 1
  • Wen-hua Ling
    • 2
  • Bin Yu
    • 1
  1. 1.Research Center of Nutrition and Food Safety, Chongqing Key Laboratory of Nutrition and Food SafetyThe Third Military Medical UniversityChongqingPeople’s Republic of China
  2. 2.Department of Medical Nutriology, School of Public Health Sun Yat-Sen UniversityGuangzhouPeople’s Republic of China

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