Cell Biochemistry and Biophysics

, Volume 60, Issue 3, pp 225–229

Effect of Zymosan on Antioxidant and Immune Function of S180 Tumor-Bearing Mice

  • Xiao-li Liu
  • Ning Lin
  • Deng Zan
  • Jing-Jue Yuan
  • Dong-Lian Cai
Original Paper
  • 148 Downloads

Abstract

The objective is to study the effect of zymosan on antioxidant and immune function of S180 tumor-bearing mice. Seventy Kunming mice were randomly divided into seven groups: a normal control group (NC), a tumor control group (TC), three dose groups of zymosan (low, medium, high), a cyclophosphamide (Cy) group, and a combination of zymosan and Cy group. The S180 tumor-bearing mice model was established by the inoculation of cancer cell suspension subcutaneously in the mouse’s right anterior limb. At the 19th day, malondialdehyde (MDA), glutathione peroxidase (GSH-Px), and superoxide dismutase (SOD) activity in liver homogenate were analyzed. The reverse transcriptase-polymerase chain reaction was used to determine the mRNA expression levels of IL-2, TNF-α, and TGF-β1. The activity of GSH-Px and SOD in the liver increased with the dose of zymosan, whereas the activity of MDA significantly decreased in the higher-dose groups of zymosan, compared to the TC group (P < 0.01). In the zymosan groups, mRNA expression levels in tissues of S180 tumor-bearing mice were significantly higher for TNF-α and IL-2, but lower for TGFβ1 than in the Cy or TC group (P < 0.01). The high-dose of zymosan markedly showed a depressant effect on S180 tumor, enhanced by the action of Cy that increased mRNA expression levels of TNF-α and IL-2. The mechanism of zymosan on the inhibition of tumor growth may be due to its ability to enhance the antioxidant and immune function in a dose-dependent manner.

Keywords

Zymosan Tumor Antioxidant Immunity 

Abbreviations

Cy

Cyclophosphamide

GSH-Px

Glutathione peroxidase

H

High-dose

L

Low-dose

M

Medium-dose

MDA

Malondialdehyde

NC

Normal control

RT-PCR

Reverse transcriptase-polymerase chain reaction

SD

Standard deviation

SOD

Superoxide dismutase

TC

Tumor control

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Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Xiao-li Liu
    • 1
  • Ning Lin
    • 1
    • 2
  • Deng Zan
    • 1
  • Jing-Jue Yuan
    • 1
  • Dong-Lian Cai
    • 1
  1. 1.Department of Clinical Nutrition, Changhai HospitalThe First Affiliated Hospital of The Second Military Medical UniversityShanghaiChina
  2. 2.Department of Clinical NutritionThe Military General Hospital of Chengdu PLAChengduChina

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