Cardiovascular Toxicology

, Volume 19, Issue 1, pp 72–81 | Cite as

Anti-fibrotic Actions of Roselle Extract in Rat Model of Myocardial Infarction

  • Shafreena Shaukat Ali
  • Siti Fatimah Azaharah Mohamed
  • Nur Hafiqah Rozalei
  • Yap Wei Boon
  • Satirah ZainalabidinEmail author


Heart failure-associated morbidity and mortality is largely attributable to extensive and unregulated cardiac remodelling. Roselle (Hibiscus sabdariffa) calyces are enriched with natural polyphenols known for antioxidant and anti-hypertensive effects, yet its effects on early cardiac remodelling in post myocardial infarction (MI) setting are still unclear. Thus, the aim of this study was to investigate the actions of roselle extract on cardiac remodelling in rat model of MI. Male Wistar rats (200–300 g) were randomly allotted into three groups: Control, MI, and MI + Roselle. MI was induced with isoprenaline (ISO) (85 mg/kg, s.c) for two consecutive days followed by roselle treatment (100 mg/kg, orally) for 7 days. Isoprenaline administration showed changes in heart weight to body weight (HW/BW) ratio. MI was especially evident by the elevated cardiac injury marker, troponin-T, and histological observation. Upregulation of plasma levels and cardiac gene expression levels of inflammatory cytokines such as interleukin (IL)-6 and IL-10 was seen in MI rats. A relatively high percentage of fibrosis was observed in rat heart tissues with over-expression of collagen (Col)-1 and Col-3 genes following isoprenaline-induced MI. On top of that, cardiomyocyte areas were larger in heart tissues of MI rats with upregulation of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) gene expression, indicating cardiac hypertrophy. Interestingly, roselle supplementation attenuated elevation of plasma troponin-T, IL-6, IL10, and gene expression level of IL-10. Furthermore, reduction of cardiac fibrosis and hypertrophy were observed. In conclusion, roselle treatment was able to limit early cardiac remodelling in MI rat model by alleviating inflammation, fibrosis, and hypertrophy; hence, the potential application of roselle in early adjunctive treatment to prevent heart failure.


Roselle Isoprenaline Fibrosis Inflammation Hypertrophy Cardiac remodelling 



This project was funded by Malaysian Toray Science Foundation (MTSF) under the Grant Code NN-2016-082 and Geran Universiti Penyelidikan under Grant Code GUP-2017-018.

Compliance with Ethical Standards

Conflict of interest

The authors declare that there is no conflict of interest regarding the publication of this paper.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Shafreena Shaukat Ali
    • 1
  • Siti Fatimah Azaharah Mohamed
    • 1
  • Nur Hafiqah Rozalei
    • 1
  • Yap Wei Boon
    • 1
  • Satirah Zainalabidin
    • 1
    Email author
  1. 1.Biomedical Science, School of Diagnostic Sciences & Applied Health, Faculty of Health SciencesUniversiti Kebangsaan MalaysiaKuala LumpurMalaysia

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