Cardiovascular Toxicology

, 11:341

Infrasound Exposure Induces Apoptosis of Rat Cardiac Myocytes by Regulating the Expression of Apoptosis-Related Proteins

  • Zhao-Hui Pei
  • Bao-Ying Chen
  • Ru Tie
  • Hai-Feng Zhang
  • Ge Zhao
  • Ping Qu
  • Xiao-Xing Zhu
  • Miao-Zhang Zhu
  • Jun Yu
Article

Abstract

It has been reported that exposure to infrasound causes cardiac dysfunction. Allowing for the key role of apoptosis in the pathogenesis of cardiovascular diseases, the objective of this study was to investigate the apoptotic effects of infrasound. Cardiac myocytes cultured from neonatal rats were exposed to infrasound of 5 Hz at 130 dB. The apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling. Also, the expression levels of a series of apoptosis-related proteins were detected. As a result, infrasound induced apoptosis of cultured rat cardiac myocytes in a time-dependant manner. The expression of proapoptotic proteins such as Bax, caspase-3, caspase-8, caspase-9, and FAS was significantly up-regulated, with concomitant down-regulated expression of antiapoptotic proteins such as Bcl-x, and the inhibitory apoptosis proteins family proteins including XIAP, cIAP-1, and cIAP-2. The expression of poly (ADP-ribose) polymerase and β-catenin, which are the substrate proteins of caspase-3, was significantly decreased. In conclusion, infrasound is an apoptotic inducer of cardiac myocytes.

Keywords

Infrasound Apoptosis Cardiac myocytes Bcl-x IAPs Caspase 

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Zhao-Hui Pei
    • 1
  • Bao-Ying Chen
    • 2
  • Ru Tie
    • 3
  • Hai-Feng Zhang
    • 3
  • Ge Zhao
    • 4
  • Ping Qu
    • 3
  • Xiao-Xing Zhu
    • 5
  • Miao-Zhang Zhu
    • 6
  • Jun Yu
    • 3
  1. 1.Department of CardiologyThird HospitalNanchangChina
  2. 2.Department of RadiologyTangdu Hospital, Fourth Military Medical UniversityXi’anChina
  3. 3.Center of Teaching ExperimentSchool of Basic Medical Science, Fourth Military Medical UniversityXi’anChina
  4. 4.Department of AnesthesiologyThe First Affiliated Hospital, School of Medicine, Xi’an Jiaotong UniversityXi’anChina
  5. 5.Department of PharmacologyFourth Military Medical UniversityXi’anChina
  6. 6.Department of PhysiologyFourth Military Medical UniversityXi’anChina

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