Cardiovascular Toxicology

, 11:341 | Cite as

Infrasound Exposure Induces Apoptosis of Rat Cardiac Myocytes by Regulating the Expression of Apoptosis-Related Proteins

  • Zhao-Hui Pei
  • Bao-Ying Chen
  • Ru Tie
  • Hai-Feng Zhang
  • Ge Zhao
  • Ping Qu
  • Xiao-Xing Zhu
  • Miao-Zhang Zhu
  • Jun Yu
Article

Abstract

It has been reported that exposure to infrasound causes cardiac dysfunction. Allowing for the key role of apoptosis in the pathogenesis of cardiovascular diseases, the objective of this study was to investigate the apoptotic effects of infrasound. Cardiac myocytes cultured from neonatal rats were exposed to infrasound of 5 Hz at 130 dB. The apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling. Also, the expression levels of a series of apoptosis-related proteins were detected. As a result, infrasound induced apoptosis of cultured rat cardiac myocytes in a time-dependant manner. The expression of proapoptotic proteins such as Bax, caspase-3, caspase-8, caspase-9, and FAS was significantly up-regulated, with concomitant down-regulated expression of antiapoptotic proteins such as Bcl-x, and the inhibitory apoptosis proteins family proteins including XIAP, cIAP-1, and cIAP-2. The expression of poly (ADP-ribose) polymerase and β-catenin, which are the substrate proteins of caspase-3, was significantly decreased. In conclusion, infrasound is an apoptotic inducer of cardiac myocytes.

Keywords

Infrasound Apoptosis Cardiac myocytes Bcl-x IAPs Caspase 

Notes

Acknowledgments

This work was supported by the National Natural Science Foundation of China (No. 30870579, 30870902, 30801385), Special grade of the financial support from China Postdoctoral Science Foundation (No. 200801266), New Medicine Research Fund (No. 2009ZX09103-671), and Natural Science Fund of Shaan’xi Province (No. 2010JM4050).

References

  1. 1.
    Leventhall, G. (2007). What is infrasound? Progress in Biophysics and Molecular Biology, 93(1–3), 130–137.PubMedCrossRefGoogle Scholar
  2. 2.
    Liu, Z. H., Chen, J. Z., Tang, Y., Chen, D., Ding, G. R., Liu, J., et al. (2004). Effects of infrasound on changes of intracellular calcium ion concentration and on expression of RyRs in hippocampus of rat brain. Journal of Low Frequency Noise, Vibration and Active Control, 23, 159–165.Google Scholar
  3. 3.
    Liu, J., Lin, T., Yan, X., Jiang, W., Shi, M., Ye, R., et al. (2010). Effects of infrasound on cell proliferation in the dentate gyrus of adult rats. Neuroreport, 21(8), 585–589.PubMedCrossRefGoogle Scholar
  4. 4.
    Dang, W. M., Wang, S., Tian, S. X., Chen, B., Sun, F., Li, W., et al. (2007). Effects of infrasound on activities of 3beta hydroxysteroid dehydrogenase and acid phosphatase of polygonal cells in adrenal cortex zona fasciculate in mice. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi, 25(2), 91–95.PubMedGoogle Scholar
  5. 5.
    Zhuang, Z., Pei, Z., & Chen, J. (2007). Infrasound-induced changes on sexual behavior in male rats and some underlying mechanisms. Environmental Toxicology and Pharmacology, 23, 111–114.PubMedCrossRefGoogle Scholar
  6. 6.
    Gordeladze, A. S., Glinchikov, V. V., & Usenko, V. R. (1986). Experimental myocardial ischemia caused by infrasound. Gigiena truda i professional’nye zabolevaniia, 6, 30–33.PubMedGoogle Scholar
  7. 7.
    Alekseev, S. V., Glinchikov, V. V., & Usenko, V. R. (1983). Myocardial ischemia in rats exposed to infrasound. Gigiena truda i professional’nye zabolevaniia, 8, 34–38.PubMedGoogle Scholar
  8. 8.
    Pei, Z., Sang, H., Li, R., Xiao, P., He, J., Zhuang, Z., et al. (2007). Infrasound-induced hemodynamics, ultrastructure, and molecular changes in the rat myocardium. Environmental Toxicology, 22(2), 169–175.PubMedCrossRefGoogle Scholar
  9. 9.
    Pei, Z., Zhuang, Z., Xiao, P., Chen, J., Sang, H., Ren, J., et al. (2009). Influence of infrasound exposure on the whole L-type calcium currents in rat ventricular myocytes. Cardiovascular Toxicology, 9(2), 70–77.PubMedCrossRefGoogle Scholar
  10. 10.
    Lee, Y., & Gustafsson, A. B. (2009). Role of apoptosis in cardiovascular disease. Apoptosis, 14(4), 536–548.PubMedCrossRefGoogle Scholar
  11. 11.
    Lü, S. Y., Zhu, M. Z., Wang, D. S., Yu, J., Guo, H. T., Hu, Y. Z., et al. (2004). Down-regulation of C-type natriuretic peptide receptor by vasonatrin peptide in cardiac myocytes and fibroblasts. Acta Pharmacologica Sinica, 25(4), 424–430.PubMedGoogle Scholar
  12. 12.
    Tuo, Q. H., Liang, L., Zhu, B. Y., Cao, X., & Liao, D. F. (2008). Effect of Daxx on cholesterol accumulation in hepatic cells. World Journal of Gastroenterology, 14(3), 435–440.PubMedCrossRefGoogle Scholar
  13. 13.
    Yu, J., Bulk, E., Ji, P., Hascher, A., Tang, M., Metzger, R., et al. (2010). The EPHB6 receptor tyrosine kinase is a metastasis suppressor that is frequently silenced by promoter DNA hypermethylation in non-small cell lung cancer. Clinical Cancer Research, 16(8), 2275–2283.PubMedCrossRefGoogle Scholar
  14. 14.
    Tiberio, R., Marconi, A., Fila, C., Fumelli, C., Pignatti, M., Krajewski, S., et al. (2002). Keratinocytes enriched for stem cells are protected from anoikis via an integrin signaling pathway in a Bcl-2 dependent manner. FEBS letters, 524(1–3), 139–144.PubMedCrossRefGoogle Scholar
  15. 15.
    Alenzi, F. Q., Lotfy, M., & Wyse, R. (2010). Swords of cell death: Caspase activation and regulation. Asian Pacific Journal of Cancer Prevention, 11(2), 271–280.PubMedGoogle Scholar
  16. 16.
    Fukuda, K. (1999). Apoptosis-associated cleavage of beta-catenin in human colon cancer and rat hepatoma cells. International Journal of Biochemistry and Cell Biology, 31(3–4), 519–529.PubMedCrossRefGoogle Scholar
  17. 17.
    Chaitanya, G. V., Steven, A. J., & Babu, P. P. (2010). PARP-1 cleavage fragments: Signatures of cell-death proteases in neurodegeneration. Cell Communication and Signaling, 8, 31.PubMedCrossRefGoogle Scholar
  18. 18.
    Gyrd-Hansen, M., & Meier, P. (2010). IAPs: From caspase inhibitors to modulators of NF-kappaB, inflammation and cancer. Nature Reviews Cancer, 10(8), 561–574.PubMedCrossRefGoogle Scholar
  19. 19.
    Strasser, A., Jost, P. J., & Nagata, S. (2009). The many roles of FAS receptor signaling in the immune system. Immunity, 30(2), 180–192.PubMedCrossRefGoogle Scholar
  20. 20.
    Bao, Y., Fan, J. Z., Li, K., Li, C., & Yang, J. F. (2008). Effects of infrasound therapy on proliferation, apoptosis and ultrastructure of human B lymphoma Raji cells. Nan Fang Yi Ke Da Xue Xue Bao, 28(6), 1093–1095.PubMedGoogle Scholar
  21. 21.
    Liu, Z. H., Chen, J. Z., Ye, L., Liu, J., Qiu, J. Y., Xu, J., et al. (2010). Effects of infrasound at 8 Hz 90 dB/130 dB on NMDAR1 expression and changes in intracellular calcium ion concentration in the hippocampus of rats. Molecular medicine reports, 3(6), 917–921.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Zhao-Hui Pei
    • 1
  • Bao-Ying Chen
    • 2
  • Ru Tie
    • 3
  • Hai-Feng Zhang
    • 3
  • Ge Zhao
    • 4
  • Ping Qu
    • 3
  • Xiao-Xing Zhu
    • 5
  • Miao-Zhang Zhu
    • 6
  • Jun Yu
    • 3
  1. 1.Department of CardiologyThird HospitalNanchangChina
  2. 2.Department of RadiologyTangdu Hospital, Fourth Military Medical UniversityXi’anChina
  3. 3.Center of Teaching ExperimentSchool of Basic Medical Science, Fourth Military Medical UniversityXi’anChina
  4. 4.Department of AnesthesiologyThe First Affiliated Hospital, School of Medicine, Xi’an Jiaotong UniversityXi’anChina
  5. 5.Department of PharmacologyFourth Military Medical UniversityXi’anChina
  6. 6.Department of PhysiologyFourth Military Medical UniversityXi’anChina

Personalised recommendations