Study on Trace Elements Concentration in Medicinal Plants Using EDXRF Technique

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Ten medicinal plants used as ingredients of folklore and Ayurvedic medicine belonging to the Malnad Kerala region of South India, were analysed for concentrations of trace elements Fe, Cr, Mn, Co, Ni, Cu, Zn, As, and Pb using the Energy Dispersive X-ray Fluorescence (EDXRF) technique. The soil from the root area of these medicinal plants was also analysed, and the soil to plant transfer factor (TF) of the elements was estimated. Iron (Fe) recorded higher concentration than the other trace elements estimated in the medicinal plants, followed by manganese (Mn) and zinc (Zn). The mean TF was found to be highest for Zn. Plectranthus amboinicus (Lour.) Spreng recorded the maximum value of TF for Zn. Most of the essential elements were found to be present in Centella asiatica (L.) Urban and indicated its medicinal importance. The data obtained in the present investigation may add up to the trace elemental database of medicinal plants in the world.

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  1. 1.

    Kamboj VP (2000) Herbal medicine. Curr Sci 78:35–51

  2. 2.

    Neverova OA, Egorova IN (2013) Assessment of heavy metal pollution of medicinal plants Taraxacum Officinale web. Growing in the Kuznetsk Basin Areas Affected by Coal Production 23(5):650–655

  3. 3.

    World Health Organisation. (1996). Trace elements in human nutrition and health, report of a WHO expert committee, W.H.O., Geneva

  4. 4.

    Rajurkar NS, Pardeshi BM (1997) Analysis of some herbal plants from India used in the Control of diabetes mellitus by NAA and AAS techniques. Appl Radiat Isot 48(8):1059–1062

  5. 5.

    Lokhande R (2010) Study of some Indian medicinal plants by application of INAA and AAS techniques. Nat Sci 02(01):26–32

  6. 6.

    Chandrashekara K, Somashekarappa HM (2016b) 210Po and 210Pb in medicinal plants in the region of Karnataka, southern India. J Environ Radioact 160:87–92

  7. 7.

    Bhabha Atomic Research Centre (BARC). (2008). Annual Report. Standard protocol for evaluation of environmental transfer factors around NPP sites

  8. 8.

    Chandrashekara K, Somashekarappa HM (2016a) Estimation of radionuclides concentration and average annual committed effective dose due to ingestion for some selected medicinal plants of South India. J Radiat Res Appl Sci 9:68–77

  9. 9.

    EML Procedure Manual. (1983). Edited by Herbert L. Volchok & Gail de Planque, Environmental Measurement Laboratory, New York

  10. 10.

    Iyengar M.A.R., Ganapathy, S., Kannan V., Rajan, M.P., and Rajaram, S. (1990). Procedure Manual. In: Workshop on Environmental Radioactivity, Kaiga, India, April 16–18

  11. 11.

    Chandrashekara K, Somashekarappa HM, Radhakrishna AP (2019) Disequilibrium of uranium series radionuclides in soil and plants of South India. J Radioanal Nucl Chem 320(2):491–501

  12. 12.

    Dutta RK, Sarkar S, Ram SS, Sudarshan M, Acharya R, Reddy AVR (2014) Applications of EDXRF and INAA techniques for studying impact of industries to the environment. J Radioanal Nucl Chem 302(3):1519–1523

  13. 13.

    Ravisankar R, Naseerutheen A, Chandrasekaran A, Bramha SN, Kanagasabapathy KV, Prasad M, Satpathy KK (2014) Energy dispersive X-ray fluorescence analysis of ancient potteries from Vellore District Tamilnadu, India with statistical approach. J Radiat Res Appl Sci 7(1):44–54

  14. 14.

    Eisenbud M. (1987). Environmental radioactivity from natural, industrial, and military sources (3rdEd.) academic press, INC.

  15. 15.

    International Atomic Energy Agency. (2006). Classification of soil systems on the basis of transfer factors or radionuclides from soil to reference plants. IAEA-TECDOC1497. Vienna

  16. 16.

    Beard JL (2001) Iron-deficiency anaemia: re-examining the nature and magnitude of the public health problem. J Nutr 131:568S–580S

  17. 17.

    Desideri D, Meli MA, Roselli C (2010) Determination of essential and non-essential elements in some medicinal plants by polarised X ray fluorescence spectrometer (EDPXRF). Michrochemical Journal 95:174–180

  18. 18.

    Olujimi OO, Bamgbose O, Arowolo T, Steiner O, Goessler W (2014) Elemental profiles of herbal plants commonly used for cancer therapy in Ogun state, Nigeria. Part I. Microchem J 117:233–241

  19. 19.

    Oladipo MO, Njinga RL, Baba A, Muhammad HL (2012) Evaluation of trace elements in some northern-Nigeria traditional medicinal plants using INAA technique. Appl Radiat Isot 70(6):917–921

  20. 20.

    Shirin K, Imad S, Shafiq S, Fatima K (2010) Determination of major and trace elements in the indigenous medicinal plant Withania somnifera and their possible correlation with therapeutic activity. Journal of Saudi Chemical Society 14(1):97–100

  21. 21.

    Linder MC, Hazegh-Azam M (1996) Copper biochemistry and molecular biology. Am J Clin Nutr 63(5):797S–811S

  22. 22.

    Winiarska-Mieczan A (2014) Cadmium, Lead, copper and zinc in breast Milk in Poland. Biol Trace Elem Res 157:36–44

  23. 23.

    Cruz KJC, de Oliveira ARS, Morais JBS, Severo JS, Mendes PMV, Melo SRS, Sousa GS, Marrero DN (2018) Zinc and insulin resistance: biochemical and molecular aspects. Biol Trace Elem Res 186:407–412

  24. 24.

    Karunakara N, Somashekarappa HM, Narayana Y, Avadhani DN, Mahesh HM, Siddappa K (2003) 226Ra, 40K and 7Be activity concentrations in plants in the environment of Kaiga, India. J Environ Radioact 65(3):255–266

  25. 25.

    James JP, Dileep BN, Ravi PM, Joshi RM, Ajith TL, Hegde AG, Sarkar PK (2011) Soil to leaf transfer factor for the radionuclides 226Ra, 40K, 137Cs and 90Sr at Kaiga region, India. J Environ Radioact 102(12):1070–1077

  26. 26.

    Karunakara N, Rao C, Ujwal P, Yashodhara I, Kumara S, Ravi PM (2013) Soil to rice transfer factors for 226Ra, 228Ra, 210Pb, 40K and 137Cs: a study on rice grown in India. J Environ Radioact 118:80–92

  27. 27.

    Olowoyo JO, Okedeyi OO, Mkolo NM, Lion GN, Mdakane STR (2012) Uptake and translocation of heavy metals by medicinal plants growing around a waste dump site in Pretoria, South Africa. S Afr J Bot 78:116–121

  28. 28.

    Zurera G, Moreno R, Salmeron J, Pozo R (1989) Heavy metal uptake from green house border soils for edible vegetables. Journal of Food Science and Agriculture 49:307–314

  29. 29.

    Mrittunjai S, Lena QM, Santos JAG (2006) Three new arsenic hyperaccumulating ferns. Sci Total Environ 364:24–31

  30. 30.

    Luongo T, Ma LQ (2005) Characteristics of arsenic accumulation by Pteris and non- Pteris ferns. Plant Soil 277:117–126

  31. 31.

    Tu C, Ma LQ, Bondada B (2002) Arsenic accumulation in the hyperaccumulator Chinese brake and its utilization potential for phytoremediation. J Environ Qual 31(5):1671–1675

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The first author is grateful to the University Grants Commission (UGC), New Delhi, for awarding a fellowship under its Faculty Development Programme.

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Correspondence to Chandrashekara Kulal.

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Kulal, C., Padhi, R.K., Venkatraj, K. et al. Study on Trace Elements Concentration in Medicinal Plants Using EDXRF Technique. Biol Trace Elem Res (2020) doi:10.1007/s12011-020-02037-7

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  • Medicinal plants
  • Trace elements
  • Folklore medicine
  • Soil to plant transfer factor