The Effects of Low Selenium on DNA Methylation in the Tissues of Chickens
- 9 Downloads
DNA methylation is involved in epigenetic mechanisms associated with gene suppression, and its abnormalities lead to gene instability and disease development. As an essential trace element in humans and animals, selenium (Se) is also associated with abnormal changes in DNA methylation. However, the effect of low Se on DNA methylation in avian tissues has not been reported. In the current study, chickens were fed a low-Se diet (0.033 mg Se/kg) or supplemented with 0.15 mg Se/kg as selenite for up to 55 days. DNA methylation levels were examined by high-performance liquid chromatography (HPLC). DNA methyltransferases (DNMTs) and methyl-DpG-binding domain protein 2 (MBD2) mRNA levels were examined through the applications of RT-PCR. The experiment aims to explore the relationship between low Se and DNA methylation. The results showed that total DNA methylation levels in the muscle tissues, brain, immune tissues, and liver of the low-selenium diet group were decreased compared with the control group. The degree of DNA methylation reduction in different tissues from largest to smallest was liver > cerebellum > thymus > brain > spleen ≥ leg muscles > pectoral muscles > bursa of Fabricius > thalamus > wing muscles. DNMT1, DNMT3A, and DNMT3B mRNA expression levels of the low-selenium diet group were decreased compared with those in the control group. The mRNA expression of the MBD2 gene was increased. The results indicate that low Se can reduce the DNA methylation levels of tissues, especially within the liver. These conclusions provide a basis for exploring the pathogenesis of selenium deficiency from the perspective of DNA methylation and create a new basis for comparative medicine.
KeywordsChicken Selenium (Se) deficiency DNA methylation DNA methyltransferases (DNMTs) Methyl-DpG-binding domain protein 2 (MBD2)
This study was supported by the International (Regional) Cooperation and Exchange Projects of the National Natural Science Foundation of China (31320103920) and the National Natural Science Foundation of China (31772814).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 3.Kaur R, Ghanghas P, Rastogi P, Kaushal N (2018) Protective role of selenium against hemolytic anemia is mediated through redox modulation. Biol Trace Elem Res 1–11. https://doi.org/10.1007/s12011-018-1483-y
- 7.Whanger PD, Weswig PH, Oldfield JE, Cheeke PR, Schmitz JA (1976) Selenium and white muscle disease in lambs: effects of vitamin E and ethoxyquin. Nutr Rep Int 13:159–173Google Scholar
- 13.Huawei Z, Lin Y, Wen-Hsing C, Uthus EO (2011) Dietary selenomethionine increases exon-specific DNA methylation of the p53 gene in rat liver and colon mucosa. J Nutr 141(8):1464–1468Google Scholar
- 21.Metes-Kosik N, Luptak I, Dibello P, Handy D, Tang S, Zhi H, Qin F, Jacobsen D, Loscalzo J, Joseph J (2012) Both selenium deficiency and modest selenium supplementation lead to myocardial fibrosis in mice via effects on redox-methylation balance. Mol Nutr Food Res 56(12):1812–1824PubMedPubMedCentralGoogle Scholar
- 25.Jie Y, Yuan Z, Hamid S, Cai J, Qi L, Hao L, Zhao R, Hong W, Xu S, Zhang Z (2017) Interplay between autophagy and apoptosis in selenium deficient cardiomyocytes in chicken. J Inorg Biochem 170:17–25Google Scholar
- 30.Kumbhar S, Khan AZ, Parveen F, Nizamani ZA, Siyal FA, El-Hack MEA, Gan F, Liu Y, Hamid M, Nido SA (2018) Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperature. AMB Express 8(1):112PubMedPubMedCentralGoogle Scholar
- 34.Tian F, Luo J, Zhang H, Chang S, Song J (2012) Marek’s disease virus challenge induced immune-related gene expression and chicken repeat 1 (CR1) methylation alterations in chickens. Am J Mol Biol 2(3):232–241Google Scholar
- 35.Richardson B, Scheinbart L, Strahler J, Gross L, Hanash S, Johnson M (2014) Evidence for impaired T cell DNA methylation in systemic lupus erythematosus and rheumatoid arthritis. Arthritis Rheum 33(11):1665–1673Google Scholar
- 37.Bermingham EN, Bassett SA, Young W, Roy NC, Mcnabb WC, Cooney JM, Di TB, Laing WA, Barnett MP (2013) Post-weaning selenium and folate supplementation affects gene and protein expression and global DNA methylation in mice fed high-fat diets. BMC Med Genet 6(1):1–18Google Scholar
- 41.Jr CG, Scott ML (1974) Antioxidant effects on selenium and vitamin E function in the chick. J Nutr 104(10):1297Google Scholar
- 43.Sun L, Huang J, Deng J, Lei X (2018) Avian selenogenome: response to dietary Se and vitamin E deficiency and supplementation. Poult Sci. https://doi.org/10.3382/ps/pey408
- 48.Dagar V, Hutchison W, Muscat A, Krishnan A, Hoke D, Buckle A, Siswara P, Amor D, Mann J, Pinner J, Colley A, Wilson M, Sachdev R, McGillivray G, Edwards M, Kirk E, Collins F, Jones K, Taylor J, Hayes I, Thompson E, Barnett C, Haan E, Freckmann M, Turner A, White S, Kamien B, Ma A, Mackenzie F, Baynam G, Kiraly-Borri C, Field M, Dudding-Byth T, Algar E (2018) Genetic variation affecting DNA methylation and the human imprinting disorder, Beckwith-Wiedemann syndrome. Clin Epigenetics 10(1):114PubMedPubMedCentralGoogle Scholar
- 51.Saito Y, Kanai Y, Sakamoto M, Saito H, Ishii H, Hirohashi S (2002) Overexpression of a splice variant of DNA methyltransferase 3b, DNMT3b4, associated with DNA hypomethylation on pericentromeric satellite regions during human hepatocarcinogenesis. Proc Natl Acad Sci U S A 99(15):10060–10065PubMedPubMedCentralGoogle Scholar
- 54.Xu L, Sun W, Jia A, Qiu L, Xiao B, Mu L, Li J, Zhang X, Wei Y, Peng C, Zhang D, Xiang X (2018) MBD2 regulates differentiation and function of Th17 cells in neutrophils- dominant asthma via HIF-1α. J Inflamm (Lond) 15:15Google Scholar