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The protective effect of selenium on the lipopolysaccharide-induced oxidative stress and depressed gene expression related to milk protein synthesis in bovine mammary epithelial cells

  • Boqi Zhang
  • Yongmei Guo
  • Sumei YanEmail author
  • Xiaoyu Guo
  • Yanli Zhao
  • Binlin Shi
Article
  • 23 Downloads

Abstract

The objective of this study was to determine the effects of selenium (Se) on antioxidative function and the synthesis of milk protein in bovine mammary epithelial cells (BMECs). Two experiments were conducted using a single-factor completely randomized design study. In part I, BMECs were randomly divided into seven groups: control (without Se) and six Se treatments (10, 20, 50, 100, 150, and 200 nmol/L). In part II, based on the results of part I, we used lipopolysaccharide (LPS) as the induced stress source to analyze the protective effect of Se on LPS-induced oxidative damage and the influence on milk protein synthesis of BMECs. BMECs were randomly divided into eight groups: control (without Se and LPS), LPS treatment (only LPS), and six Se treatments with LPS (LS10 to LS200). Treatment of BMECs with Se was found to significantly improve cell proliferation and antioxidant function. LPS could induce oxidative damage which significantly inhibited cell proliferation and antioxidant function in BMECs. Se had a protective effect on the oxidative damage of BMECs induced by LPS. Additionally, our results indicated that LPS damage downregulated the gene expression of milk protein synthesis. Se effectively relieved the inhibition due to LPS-induced oxidative damage on the synthesis of milk protein, and Se concentrations of 50 to 200 nmol/L showed the best effect. In conclusion, Se at concentrations of 50 to 100 nmol/L is better for antioxidant function but had no effect on milk protein synthesis in healthy BMECs. Se ameliorated the damage caused by LPS-induced by improving levels of antioxidant markers and upregulating milk protein synthesis and the expression of genes associated with milk protein in BMECs.

Keywords

Selenium Bovine mammary epithelial cells Milk protein Lipopolysaccharide 

Notes

Acknowledgments

The authors are grateful to Yongmei Guo, Xiaoyu Guo and Yanli Zhao for their assistance during the experiments.

Funding Information

This study was supported by the National Natural Science Foundation of China (Project No. 31560650).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Inner Mongolia Key Laboratory of Animal Nutrition and Feed Science, College of Animal ScienceInner Mongolia Agricultural UniversityHohhotChina

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