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Comparison and Risk Assessment for Trace Heavy Metals in Raw Pu-erh Tea with Different Storage Years

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Abstract

This research conducted an exploration of the content of microelements (As, Cr, Cd, Pb, Cu, Zn, Mn, and Hg) in raw Pu-erh tea with different storage years. The contents of As, Cr, Cd, Pb, Cu, Zn, Mn, and Hg were 0.14, 0.82, 0.02, 0.52, 14.59, 33.51, 564.02, and 0.01 μg/g, respectively, and were all less than the national standard limit values in China. The target hazard quotients (THQs) of each heavy metal were all lower than 1, and the value of combined risk hazard index (HI) of all to adults was 0.221, which presents no health risk when consumed properly by adults of the raw Pu-erh tea infusions. Interestingly, there was no significant correlation between the heavy metal element (As, Cr, Cd, Pb, Cu, Zn, Mn, and Hg) contents and the THQ values of raw Pu-erh tea samples and storage years; the correlation coefficients (R2) range from 0.01 to 0.33 and from 0.01 to 0.57, respectively. The result showed that the storage years showed no effect on the exposure risk of heavy metals; the heavy metal elements in tea samples come from the atmosphere and soil.

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References

  1. State Administration for Quality Supervision and Inspection and Quarantine (SAQSIQ) (2008) Product of geographical indication Pu-erh tea; GB/T. Beijing, China Standards Press, SAQSIQ, pp 22111–22008

    Google Scholar 

  2. Liang Z, Tian XJ, Luo LY, Guan XL, Gao LR (2017) Characteristic components of aqueous extracts of raw Pu-erh tea with different storage times. Food Sci 38:198–205

    Google Scholar 

  3. Qiang XZ, Li LY, Ming B, Jie ZH (2012) Research progress of health care efficacy of Pu-erh tea. China Heal Nutr 22:2449–2451

    Google Scholar 

  4. Lee LK, FOO KY (2013) Recent advances on the beneficial use and health implications of Pu-Erh tea. Food Res Int 53:619–628

    CAS  Google Scholar 

  5. Wang BS, Yu HM, Chang LW, Yen WJ, Duh PD (2008) Protective effects of Pu-erh tea on LDL oxidation and nitric oxide generation in macrophage cells. LWT Food Sci Technol 41:1122–1132

    CAS  Google Scholar 

  6. Duh PD, Wang BS, Liou SJ, Lin CJ (2010) Cytoprotective effects of pu-erh tea on hepatotoxicity in vitro and in vivo induced by tert-butyl-hydroperoxide. Food Chem 119:580–585

    CAS  Google Scholar 

  7. Huang YW, Shao WF, Leng LY, Zhang DY (2010) Research of anti-fatigue effect of unfermented Pu-erh tea. Southwest China J Agric Sci 23:801–804

  8. Wu SC, Yen GC, Wang BS, Chiu CK, Yen WJ, Chang LW (2007) Antimutagenic and antimicrobial activities of pu-erh tea. LWT Food Sci Technol 40:506–512

    CAS  Google Scholar 

  9. Fang X, Bin LI, Chen D, Chen ZZ (2008) Review on functional components and mechanism of quality formation of Puer tea. Sci Technol Food Ind 29:313–316

    CAS  Google Scholar 

  10. Cheng HG, Zhou T, Li Q, Lu L, Lin CY (2014) Anthropogenic chromium emissions in China from 1990 to 2009. PLoS One 9:e87753

    PubMed  PubMed Central  Google Scholar 

  11. Pacyna EG, Pacyna JM, Sundseth K, Munthe J, Kindbom K, Wilson S, Steenhuisen F, Maxson P (2010) Global emission of mercury to the atmosphere from anthropogenic sources in 2005 and projections to 2020. Atmos Environ 44:2487–2499

    CAS  Google Scholar 

  12. Pacyna JM, Travnikov O, Simone FD, Hedgecock IM, Kindbom K (2016) Current and future levels of mercury atmospheric pollution on global scale. Atmos Chem Phys:16:1–16:1635

  13. Dias D, Bessa J, Guimarães S, Soares ME, Bastos ML, Teixeira HM (2016) Inorganic mercury intoxication: a case report. Forensic Sci Int 259:e20–e24

    CAS  PubMed  Google Scholar 

  14. Li L, Chen CF, Tao L, Min S (2015) Experimental study of mercury exposure-induced renal toxicity in mice. Sichuan J Phys Sci 37:10–13

    CAS  Google Scholar 

  15. Nakamura M, Hachiya N, Murata KY, Nakanishi I, Kondo T, Yasutake A, Miyamoto KI, Ping HS, Omi S, Furusawa H (2014) Methylmercury exposure and neurological outcomes in Taiji residents accustomed to consuming whale meat. Environ Int 68:25–32

    CAS  PubMed  Google Scholar 

  16. Sakamoto M, Murata K, Kakita A, Sasaki M (2011) A Review of mercury toxicity with special reference to methylmercury [M]// Environmental Chemistry and Toxicology of Mercury. John Wiley & Sons, Inc

  17. Chen C, Xun P, Nishijo M, Sekikawa A, He K (2015) Cadmium exposure and risk of pancreatic cancer: a meta-analysis of prospective cohort studies and case-control studies among individuals without occupational exposure history. Environ Sci Pollut Res Int 22:17465–17474

    CAS  PubMed  PubMed Central  Google Scholar 

  18. Cho YA, Kim J, Woo HD, Kang M (2013) Dietary cadmium intake and the risk of cancer: a meta-analysis. PLoS One 8:e75087

    CAS  PubMed  PubMed Central  Google Scholar 

  19. Jin T, Nordberg M, Frech W, Dumont X, Bernard A, Ye TT, Kong Q, Wang Z, Li P, Lundström NG (2002) Cadmium biomonitoring and renal dysfunction among a population environmentally exposed to cadmium from smelting in China (ChinaCad). Biometals 15:397–410

    CAS  PubMed  Google Scholar 

  20. Anttila S, Kokkonen P, Rainio P, Kalliom PL, Pallon J, Malmqvist K, Pakarinen P, Sutinen S (1989) High concentrations of chromium in lung tissue from lung cancer patients. Cancer 63:467

    CAS  PubMed  Google Scholar 

  21. Kuo CY, Wong RH, Lin JY, Lai JC, Lee H (2006) Accumulation of chromium and nickel metals in lung tumors from lung cancer patients in Taiwan. J Toxicol Environ Heal Part A 69:1337–1344

    CAS  Google Scholar 

  22. Nordström S, Beckman L, Nordenson I (1979) Occupational and environmental risks in and around a smelter in northern Sweden. V. Spontaneous abortion among female employees and decreased birth weight in their offspring. Hereditas 90:291

    PubMed  Google Scholar 

  23. Garza A, Vega R, Soto E (2006) Cellular mechanisms of lead neurotoxicity. Med Sci Monit 12:RA57

    PubMed  Google Scholar 

  24. Landrigan PJ, Boffetta P, Apostoli P (2000) The reproductive toxicity and carcinogenicity of lead: a critical review. Am J Ind Med 38:231–243

    CAS  PubMed  Google Scholar 

  25. Yang WY, Staessen JA (2018) Letter to editor: blood pressure, hypertension and lead exposure. Environ Health 17:16

    PubMed  PubMed Central  Google Scholar 

  26. Guha MDN (2008) Chronic arsenic toxicity & human health. Indian J Med Res 128:436

    Google Scholar 

  27. Jomova K, Jenisova Z, Feszterova M, Baros S, Liska J, Hudecova D, Rhodes CJ, Valko M (2015) Arsenic: toxicity, oxidative stress and human disease. J Appl Toxicol 31:95–107

    Google Scholar 

  28. Saha JC, Dikshit AK, Bandyopadhyay M, Saha KC (1999) A review of arsenic poisoning and its effects on human health. CRC Rev Environ Control 29:281–313

    CAS  Google Scholar 

  29. Arnold LL (2006) Methylated arsenicals: the implications of metabolism and carcinogenicity studies in rodents to human risk assessment. CRC Rev Toxicol 36:99–133

    Google Scholar 

  30. Chen HL, Lee CC, Huang WJ, Huang HT, Wu YC, Hsu YC, Kao YT (2016) Arsenic speciation in rice and risk assessment of inorganic arsenic in Taiwan population. Environ Sci Pollut Res 23:4481–4488

    CAS  Google Scholar 

  31. Nkansah MA, Opoku F, Ackumey AA (2016) Risk assessment of mineral and heavy metal content of selected tea products from the Ghanaian market. Environ Monit Assess 188:1–11

    CAS  Google Scholar 

  32. Sofuoglu SC, Kavcar P (2008) An exposure and risk assessment for fluoride and trace metals in black tea. J Hazard Mater 158:392–400

    CAS  PubMed  Google Scholar 

  33. Zhang J, Yang R, Chen R, Peng Y, Wen X, Gao L (2018) Accumulation of heavy metals in tea leaves and potential health risk assessment: a case study from Puan County, Guizhou Province, China. Int J Environ Res Public Health 15:133

    PubMed Central  Google Scholar 

  34. Fang F, Wang X, Lin Y (2015) Study on enrichment patterns and health risk of metal elements of tea in typical mountainous tea garden in South Anhui. J Soil Water Conserv 29:230–235

    Google Scholar 

  35. Cao H, Qiao L, Zhang H, Chen J (2010) Exposure and risk assessment for aluminium and heavy metals in Puerh tea. Sci Total Environ 408:2777–2784

    CAS  PubMed  Google Scholar 

  36. Cap YN, Liu TX (2011) Analysis of aroma composition in Pu-erh raw and ripe teas with different storage time. Food Ind:64–67

  37. Zhi ZL, Liang WD, Xin CW, Fen CY (2007) Determination of volatiles of Puer tea stored for different lengths of time. Acta Horticulturae sinica 34:504–506

    Google Scholar 

  38. Yang XM, Liu Y, Mu LH, Wang W, Zhang Q, Luo ML (2018) Discriminant research for identifying aromas of non-fermented Pu-erh tea from different storage years using an electronic nose. J Food Process Preserv 42:e13721

    Google Scholar 

  39. Chen L, Xiong Z, Sun H, Miao FJ (2011) Study on the relationship between the content of tea polyphenol and caffeine and the storage period of Puer teas. Sci Technol Food Ind 32:132–134

    Google Scholar 

  40. Ku KM, Kim J, Park HJ, Liu KH, Lee CH (2010) Application of metabolomics in the analysis of manufacturing type of pu-erh tea and composition changes with different postfermentation year. J Agric Food Chem 58:345–352

    CAS  PubMed  Google Scholar 

  41. Duan XH, Hui Z, Hu CM (2012) Research on ingredient changes of Pu-erh tea in different storage time. S China J Agri Sci 25:000111–000114

    Google Scholar 

  42. United States Environmental Protection Agency (USEPA) (1998) SW-846 Method 6020A: inductively coupled plasma - mass spectrometry, pp 1–22

    Google Scholar 

  43. Wu XY (2017) Studies on maximum residue limits for pesticides in tea and relative risk assessment. Anhui Agricultural University: Hefei, China: pp1

  44. Fu QL, Liu Y, Li L, Achal V (2014) A survey on the heavy metal contents in Chinese traditional egg products and their potential health risk assessment. Food Addit Contam Part B 7:99–105

    CAS  Google Scholar 

  45. United States Environmental Protection Agency (USEPA) (2007) Concepts, methods and data sources for cumulative health risk assessment of multiple chemicals, exposures and effects: a resource document. EPA/600/R-06/013F. Office of Research and Development, National Center for Environmental Assessment, Cincinnati, OH, USA

    Google Scholar 

  46. Zhang HQ, Ni BF, Tian WZ, Zhang GY, Huang DH, Liu CX, Xiao CJ, Sun HC, Zhao CJ (2011) Study on essential and toxic elements intake from drinking of Chinese tea. J Radioanal Nucl Chem 287:887–892

    CAS  Google Scholar 

  47. Li L, Fu QL, Achal V, Liu Y (2015) A comparison of the potential health risk of aluminum and heavy metals in tea leaves and tea infusion of commercially available green tea in Jiangxi, China. Environ Monit Assess 187:4445

    Google Scholar 

  48. Zhang QH, Long ZB, Lin SX, Zhu P, Tan H, Lin CH (2013) Distribution of heavy metals in soil and tea from Yunwu tea area in Guizhou Province and diffusion characteristics of heavy metals in tea infusion. Food Sci 34:212–215

    Google Scholar 

  49. Agnieszka GK, Katarzyna MK (2016) Potential health risk of selected metals for Polish consumers of oolong tea from the Fujian Province, China. Hum Ecol Risk Assess Int J 22:19

    Google Scholar 

  50. Ji XF, Zheng N, Wang Y, Liu Q, Zhang JJ (2015) Accumulation of mercury in soil-maize system of non-ferrous metals smelting area and its related risk assessment. Environ Sci 36:3845–3851

    Google Scholar 

  51. Li RZ, Pan CR, Xu JJ, Chen J, Jiang YM (2013) Contamination and health risk for heavy metals via consumption of vegetables grown in fragmentary vegetable plots from a typical nonferrous metals mine city. Environ Sci 34:1077–1085

    Google Scholar 

  52. Atafar Z, Mesdaghinia A, Nouri J, Homaee M, Yunesian M, Ahmadimoghaddam M, AH M (2010) Effect of fertilizer application on soil heavy metal concentration. Environ Monit Assess 160:83

    CAS  PubMed  Google Scholar 

  53. Jie HL, Ke ZZ (2010) The situation of heavy metal content in tea and its control measures. S China Agri 05:89–92

    Google Scholar 

  54. Yang W, Baogang LI (2011) Effect of atmospheric deposition on heavy metal accumulation in tea leaves. Sci Technol Rev 29:55–59

    Google Scholar 

  55. Wang K, Hua SB, Tian HZ, Zhu CY, Gao JJ, Wang Y, Zhou JR, Zhu JX (2015) Atmospheric emission inventory of typical heavy metals from iron and steel industry in China, 2011. China Environ Sci 35:2934–2938

    CAS  Google Scholar 

  56. Pacyna JM, Pacyna EG, Aas W (2009) Changes of emissions and atmospheric deposition of mercury, lead, and cadmium. Atmos Environ 43:113–127

    Google Scholar 

  57. Tong Y, Yin X, Lin H, Duo B, Zeng D, Wang H, Deng C, Chen L, Li J, Zhang W (2016) Recent decline of atmospheric mercury recorded by Androsace tapete on the Tibetan Plateau. Environ Sci Technol 50:acs.est.6b04632

    Google Scholar 

  58. Li F, Wang X, Luo J, Yuan W, Yu ZH, Shang LH (2017) Bioaccumulation of heavy metals in twigs and leaves of Abies fabri at Mount Gongga in China: a comparison study between 1999 and 2014. Environ Sci 38:3046–3053

    Google Scholar 

  59. National Health and Family Planning Commission of the People’s Republic of China (NHFPCPRC) and China Food and Drug Administration (CFDA) (2017) National Standard for Food Safety: limit of contaminants in food; GB2762-2017. Beijing, China Standards Press, NHFPCPRC and CFDA

    Google Scholar 

  60. Ministry of Agriculture of the People’s Republic of China (MAPRC) (2012) Green food: tea; NY/T 288-2012. Beijing, China Standards Press, MAPRC

    Google Scholar 

  61. Ministry of Agriculture of the People’s Republic of China (MAPRC) (2003) The limit of chromium, cadmium, mercury, arsenic and fluoride in tea; NY 659-2003. Beijing, China Standards Press, MAPRC

    Google Scholar 

  62. Wu CD, Zhu YC (2001) Sequential extraction of trace elements and the geological significance of fractions in black shales, West Hunan and East Guizhou. Bull Mineral Petrol Geochem 1:14–20

    Google Scholar 

  63. Wilson SJ, Steenhuisen F, Pacyna JM, Pacyna EG (2006) Mapping the spatial distribution of global anthropogenic mercury atmospheric emission inventories. Atmos Environ 40:4621–4632

    CAS  Google Scholar 

  64. Pacyna JM, Travnikov O, De Simone F, Hedgecock IM, Sundseth K, Pacyna EG, Steenhuisen F, Pirrone N, Munthe J, Kindbom K (2016) Current and future levels of mercury atmospheric pollution on a global scale. Atmos Chem Phys:16:1–16:1635

  65. Shen FM, Chen HW (2008) Element composition of tea leaves and tea infusions and its impact on health. Bull Environ Contam Toxicol 80:300–304

    CAS  PubMed  Google Scholar 

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Acknowledgments

The authors are very thankful to the State Key Laboratory of Environmental Geochemistry, Institute of Geochemistry, Chinese Academy of Sciences, Guiyang, for the use of their laboratory facilities for this research.

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This work was supported by the Project of Innovation and Application of plant germplasm Foundation of China.

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Correspondence to Bin Yang.

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Li, F., Lu, Q., Li, M. et al. Comparison and Risk Assessment for Trace Heavy Metals in Raw Pu-erh Tea with Different Storage Years. Biol Trace Elem Res 195, 696–706 (2020). https://doi.org/10.1007/s12011-019-01886-1

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