In Vitro Cytotoxicity Studies on Human Thyroid Gland Undifferentiated Carcinoma Cells Using the Nickel Complex of (Z)-3-Bromo-5-((p-tolylimino)methyl)phenol

  • Emine Gülhan BakirdereEmail author


Metal complexes for medical applications are the promising area of research and metallo-drugs have been developed and evaluated to enhance the efficiency of drugs and minimize their side effects. This study describes the synthesis of a Schiff base ligand (Z)-3-bromo-5-((p-tolylimino)methyl)phenol from the reaction of 5-bromosalicilaldehyde and p-toluidine, and its subsequent complexation with nickel. Characterization and cell viability studies were performed for both Schiff base ligand and its metal complex with 1H-NMR, FTIR, TG analysis, and UV–VIS spectrometry. With the aim of proving the Ni complex formation, quantitative analysis was done with flame atomic absorption spectrometry. The recovery result obtained for the formed complex was 103.9%. The cell viability of human thyroid gland undifferentiated carcinoma cells (8305C) treated with the Ni-ligand complex was determined as 12.7 ± 0.17%. It was observed that the nickel complex could be a significant anticancer agent as tested by the MTT assay method.

Graphical Abstract



FAAS 1H-NMR IR MTT assay Nickel Schiff base 


Compliance with Ethical Standards

Conflict of Interest

The author declares that she has no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Science Education, Faculty of EducationYildiz Technical UniversityİstanbulTurkey

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