Status of Serum Copper, Magnesium, and Total Antioxidant Capacity in Patients with Polycystic Ovary Syndrome

  • Maryam Kanafchian
  • Sedigheh Esmaeilzadeh
  • Soleiman MahjoubEmail author
  • Maryam Rahsepar
  • Maryam Ghasemi


This study evaluates serum copper and magnesium and total antioxidant capacity levels in PCOS patients. In this regard, the probable association of copper and magnesium with total antioxidant capacity (TAC) was investigated. In total, 150 women (60 PCOS patients and 90 healthy subjects) participated in this case–control study. PCOS was diagnosed according to the Rotterdam criteria (2003). Serum Cu, Mg, Ca, TAC, insulin levels, and insulin resistance indices were determined. Insulin was measured using ELISA methods. Serum Cu and Mg levels were measured by an atomic absorption spectrophotometer and the Xylidyl Blue method respectively. The correlations between the parameters were analyzed using the Spearman correlation test. Serum Cu level was significantly higher while TAC was significantly lower in the PCOS patients than those in the controls (p = 0.019 and p = 0.002 respectively). No significant difference was detected between the two groups in terms of serum Mg and Ca levels and Ca/Mg ratio. In insulin-resistant PCOS subjects, there was a negative correlation between Mg levels and homeostatic model assessment for insulin resistance (r = − 0.449, p = 0.006) but a positive correlation between Mg levels and quantitative insulin sensitivity check index (r = 0.480, p = 0.003). A negative correlation also existed between Mg levels and TAC in non-insulin-resistant PCOS patients (r = − 0.407, p = 0.04). According to the results, copper and magnesium seem to contribute to oxidative stress and insulin resistance in PCOS patients. Therefore, to prevent long-term metabolic complications in PCOS women, it is recommended that these elements be routinely monitored. Also, significantly lower levels of serum TAC in PCOS patients than in normal women may suggest increased oxidative stress in such patients.


Polycystic ovary syndrome Insulin resistance Copper Magnesium Total antioxidant capacity 



Polycystic ovary syndrome


Total antioxidant capacity







Ca/Mg ratio

Calcium/magnesium ratio

G/I ratio

Glucose/insulin ratio


Body mass index


Fasting blood sugar


Insulin resistance


Non-insulin resistance


Homeostatic model assessment for insulin resistance


Quantitative insulin sensitivity check index



We would like to thank the participating patients as well as the staff of Fatemeh Zahra Infertility and Reproductive Health Research Center for their valuable cooperation with this project.

Funding information

We are grateful for the financial support granted by Babol University of Medical Sciences, Iran.

Compliance with Ethical Standards

Approval was obtained from the ethics committee of Babol University of Medical Sciences (MUBABOL.REC.1394.65). All the participants were informed of the aims of the study, and informed consents were obtained from them. The biochemical tests had no cost for the participants, and the results of these tests were reported to them.

Conflict of Interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Maryam Kanafchian
    • 1
  • Sedigheh Esmaeilzadeh
    • 2
  • Soleiman Mahjoub
    • 2
    Email author
  • Maryam Rahsepar
    • 1
  • Maryam Ghasemi
    • 2
  1. 1.Student Research CommitteeBabol University of Medical SciencesBabolIran
  2. 2.Infertility and Reproductive Health Research Center, Health Research InstituteBabol University of Medical SciencesBabolIran

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