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Synthesis and Biodistribution Study of Biocompatible 198Au Nanoparticles by use of Arabinoxylan as Reducing and Stabilizing Agent

  • Fozia Iram
  • Mohammad S. IqbalEmail author
  • Irfan U. Khan
  • Rashid Rasheed
  • Aqsa Khalid
  • Muhammad Khalid
  • Saira Aftab
  • Abdul R. Shakoori
Article
  • 68 Downloads

Abstract

Radioactive gold-198 is a useful diagnostic and therapeutic agent. Gold in the form of nanoparticles possesses even more exciting properties. This work aimed at arabinoxylan-mediated synthesis and biodistribution study of radioactive gold nanoparticles (198AuNPs). The particles were synthesized by mixing suspension of arabinoxylan with H198AuCl4 without use of any additional reducing and stabilizing agents. An aqueous suspension of arabinoxylan was added to a H198AuCl4 solution, which resulted in reduction of Au3+ to 198AuNPs. Biodistribution was studied in vitro and in rabbit. The particles having exceptional stability were readily formed. Highest radioactivity was recorded in spleen after 3 h followed by liver, heart, kidney, and lungs after i.v. administration. After 24 h, the activity was not detectable in the spleen; it accumulated in the liver. However, after oral administration, the activity mainly accumulated in the colon. In serum proteins, the distribution was α1-globulin 6.5%, α2-globulin ~ 2%, β-globulin ~ 1%, γ-globulin 0.7%, and albumin 0.7% of the administered dose. This indicates a low protein binding implying high bioavailability of the particles. The cytotoxicity study showed that the particles were inactive against HeLa cell line and Agrobacteriumtumefaciens. Highly stable 198AuNPs reported in this work have the potential for targeting the colon. They show affinity for globulins, the property that can be used in the study of the immune system.

Keywords

Hemicelluloses Arabinoxylan Gold nanoparticles Targeted delivery Radioactive gold nanoparticles 

Notes

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval for Use of Human Blood and Animals

The study protocols regarding use of human blood and animals were reviewed and approved by the Institutional Review Board of Forman Christian College Lahore. Written informed consent was obtained from the blood donors (healthy human adults). Guidelines provided in Good Clinical Practice by ICH, World Medical Association Declaration of Helsinki and APA Committee on Animal Research and Ethics (CARE), USA, were followed.

References

  1. 1.
    Riley RS, Day ES (2017) Gold nanoparticle-mediated photothermal therapy: applications and opportunities for multimodal cancer treatment. Wiley Interdiscip Rev Nanomed Nanobiotech 9Google Scholar
  2. 2.
    Mahan MM, Doiron AL (2018) Gold nanoparticles as X-ray, CT, and multimodal imaging contrast agents: formulation, targeting, and methodology. J Nanomater 2018:1–15CrossRefGoogle Scholar
  3. 3.
    Ruan S, Yuan M, Zhang L et al (2015) Tumor microenvironment sensitive doxorubicin delivery and release to glioma using angiopep-2 decorated gold nanoparticles. Biomaterials 37:425–435.  https://doi.org/10.1016/j.biomaterials.2014.10.007 CrossRefGoogle Scholar
  4. 4.
    Manohar N, Reynoso FJ, Diagaradjane P, Krishnan S, Cho SH (2016) Quantitative imaging of gold nanoparticle distribution in a tumor-bearing mouse using benchtop x-ray fluorescence computed tomography. Sci Rep 6:22079.  https://doi.org/10.1038/srep22079 CrossRefGoogle Scholar
  5. 5.
    Vigderman L, Zubarev ER (2013) Therapeutic platforms based on gold nanoparticles and their covalent conjugates with drug molecules. Adv Drug Deliv Rev 65:663–676.  https://doi.org/10.1016/j.addr.2012.05.004 CrossRefGoogle Scholar
  6. 6.
    Jain PK (2014) Gold nanoparticles for physics, chemistry and biology. Edited by Catherine Louis and Olivier Pluchery. Angew Chem Int Ed 53:1197–1197.  https://doi.org/10.1002/anie.201309807 CrossRefGoogle Scholar
  7. 7.
    Nam J-M, Thaxton CS, Mirkin CA et al (2009) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Nature 9:1–8.  https://doi.org/10.1016/j.toxlet.2005.10.003 Google Scholar
  8. 8.
    Her S, Jaffray DA, Allen C (2017) Gold nanoparticles for applications in cancer radiotherapy: mechanisms and recent advancements. Adv Drug Deliv Rev 109:84–101CrossRefGoogle Scholar
  9. 9.
    Austin LA, MacKey MA, Dreaden EC, El-Sayed MA (2014) The optical, photothermal, and facile surface chemical properties of gold and silver nanoparticles in biodiagnostics, therapy, and drug delivery. Arch Toxicol 88:1391–1417CrossRefGoogle Scholar
  10. 10.
    Qu X, Li Y, Li L, et al (2015) Fluorescent gold nanoclusters: synthesis and recent biological application. J Nanomater 2015:Article ID 784097Google Scholar
  11. 11.
    Ma Z, Xia H, Liu Y, Liu B, Chen W, Zhao YD (2013) Applications of gold nanorods in biomedical imaging and related fields. Chin Sci Bull 58:2530–2536.  https://doi.org/10.1007/s11434-013-5720-7 CrossRefGoogle Scholar
  12. 12.
    Jain PK, Lee KS, El-Sayed IH, El-Sayed MA (2006) Calculated absorption and scattering properties of gold nanoparticles of different size, shape, and composition: applications in biological imaging and biomedicine. J Phys Chem B 110:7238–7248.  https://doi.org/10.1021/jp057170o CrossRefGoogle Scholar
  13. 13.
    Liu X, Atwater M, Wang J, Huo Q (2007) Extinction coefficient of gold nanoparticles with different sizes and different capping ligands. Colloids Surf B: Biointerfaces 58:3–7.  https://doi.org/10.1016/j.colsurfb.2006.08.005 CrossRefGoogle Scholar
  14. 14.
    Alric C, Taleb J, Le Duc G et al (2008) Contrast agents for both X-ray computed tomography and magnetic resonance imaging. J Am Chem Soc 130:5908–5915.  https://doi.org/10.1021/ja078176p CrossRefGoogle Scholar
  15. 15.
    Norouzi H, Khoshgard K, Akbarzadeh F (2018) In vitro outlook of gold nanoparticles in photo-thermal therapy: a literature review. Lasers Med Sci 33:917–926CrossRefGoogle Scholar
  16. 16.
    Huang X, El-Sayed MA (2011) Plasmonic photo-thermal therapy (PPTT). Alexandria J Med 47:1–9.  https://doi.org/10.1016/j.ajme.2011.01.001 CrossRefGoogle Scholar
  17. 17.
    Axiak-Bechtel SM, Upendran A, Lattimer JC, Kelsey J, Cutler CS, Selting KA, Bryan JN, Henry CJ, Boote E, Tate DJ, Bryan ME, Katti KV, Kannan R (2014) Gum arabic-coated radioactive gold nanoparticles cause no short-term local or systemic toxicity in the clinically relevant canine model of prostate cancer. Int J Nanomedicine 9:5001–5011.  https://doi.org/10.2147/IJN.S67333 CrossRefGoogle Scholar
  18. 18.
    Shukla R, Chanda N, Zambre A, Upendran A, Katti K, Kulkarni RR, Nune SK, Casteel SW, Smith CJ, Vimal J, Boote E, Robertson JD, Kan P, Engelbrecht H, Watkinson LD, Carmack TL, Lever JR, Cutler CS, Caldwell C, Kannan R, Katti KV (2012) Laminin receptor specific therapeutic gold nanoparticles (198AuNP-EGCg) show efficacy in treating prostate cancer. Proc Natl Acad Sci 109:12426–12431.  https://doi.org/10.1073/pnas.1121174109 CrossRefGoogle Scholar
  19. 19.
    Chanda N, Kan P, Watkinson LD, Shukla R, Zambre A, Carmack TL, Engelbrecht H, Lever JR, Katti K, Fent GM, Casteel SW, Smith CJ, Miller WH, Jurisson S, Boote E, Robertson JD, Cutler C, Dobrovolskaia M, Kannan R, Katti KV (2010) Radioactive gold nanoparticles in cancer therapy: therapeutic efficacy studies of GA-198AuNP nanoconstruct in prostate tumor-bearing mice. Nanomedicine 6:201–209.  https://doi.org/10.1016/j.nano.2009.11.001 CrossRefGoogle Scholar
  20. 20.
    Radionuclide NR, Phillips WT, Otto RA, Bao A (2011) Interventional therapy of head and neck cancer with lipid nanoparticle-carried Rhenium-186 radionuclide. J Vasc Interv Radiol 21:1271–1279.  https://doi.org/10.1016/j.jvir.2010.02.027.Interventional Google Scholar
  21. 21.
    Al-Yasiri AY, Khoobchandani M, Cutler CS et al (2017) Mangiferin functionalized radioactive gold nanoparticles (MGF- 198 AuNPs) in prostate tumor therapy: green nanotechnology for production, in vivo tumor retention and evaluation of therapeutic efficacy. Dalton Trans 46:14561–14571.  https://doi.org/10.1039/C7DT00383H CrossRefGoogle Scholar
  22. 22.
    Tobias JS, Hochhauser D (2009) Cancer and its management. Wiley, HobokenCrossRefGoogle Scholar
  23. 23.
    Kim D, Park S, Jae HL et al (2007) Antibiofouling polymer-coated gold nanoparticles as a contrast agent for in vivo X-ray computed tomography imaging. J Am Chem Soc 129:7661–7665.  https://doi.org/10.1021/ja071471p CrossRefGoogle Scholar
  24. 24.
    Lee J, Chatterjee DK, Lee MH, Krishnan S (2014) Gold nanoparticles in breast cancer treatment: promise and potential pitfalls. Cancer Lett 347:46–53CrossRefGoogle Scholar
  25. 25.
    Hainfeld JF, Slatkin DN, Smilowitz HM (2004) The use of gold nanoparticles to enhance radiotherapy in mice. Phys Med Biol:49.  https://doi.org/10.1088/0031-9155/49/18/N03
  26. 26.
    Chen Y, Wang X (2008) Novel phase-transfer preparation of monodisperse silver and gold nanoparticles at room temperature. Mater Lett 62:2215–2218.  https://doi.org/10.1016/j.matlet.2007.11.050 CrossRefGoogle Scholar
  27. 27.
    Jeong GH, Lee YW, Kim M, Han SW (2009) High-yield synthesis of multi-branched gold nanoparticles and their surface-enhanced Raman scattering properties. J Colloid Interface Sci 329:97–102.  https://doi.org/10.1016/J.JCIS.2008.10.004 CrossRefGoogle Scholar
  28. 28.
    Deraedt C, Salmon L, Gatard S, Ciganda R, Hernandez R, Ruiz J, Astruc D (2014) Sodium borohydride stabilizes very active gold nanoparticle catalysts. Chem Commun 50:14194–14196.  https://doi.org/10.1039/c4cc05946h CrossRefGoogle Scholar
  29. 29.
    Amin M, Iram F, Iqbal MS, Saeed MZ, Raza M, Alam S (2013) Arabinoxylan-mediated synthesis of gold and silver nanoparticles having exceptional high stability. Carbohydr Polym 92:1896–1900.  https://doi.org/10.1016/j.carbpol.2012.11.056 CrossRefGoogle Scholar
  30. 30.
    Iram F, Iqbal MS, Athar MM, Saeed MZ, Yasmeen A, Ahmad R (2014) Glucoxylan-mediated green synthesis of gold and silver nanoparticles and their phyto-toxicity study. Carbohydr Polym 104:29–33.  https://doi.org/10.1016/j.carbpol.2014.01.002 CrossRefGoogle Scholar
  31. 31.
    Rabito MF, Reis AV, dos Reis Freitas A et al (2012) A pH/enzyme-responsive polymer film consisting of Eudragit® FS 30 D and arabinoxylane as a potential material formulation for colon-specific drug delivery system. Pharm Dev Technol 17:429–436.  https://doi.org/10.3109/10837450.2010.546409 CrossRefGoogle Scholar
  32. 32.
    Agarwal VK, Gupta A, Chaturvedi S, Khan F (2016) Polysaccharide: carrier in colon targeted drug delivery system. MIT Int J Pharm Sci 2:1–9Google Scholar
  33. 33.
    Lemarchand C, Gref R, Couvreur P (2004) Polysaccharide-decorated nanoparticles. Eur J Pharm Biopharm 58:327–341CrossRefGoogle Scholar
  34. 34.
    Massey S, Iqbal MS, Wolf B et al (2016) Comparative drug loading and release study on some carbohydrate polymers. Lat Am J Pharm 35:146–155Google Scholar
  35. 35.
    Weitzhandler M, Barreto V, Pohl C, Jandik P, Cheng J, Avdalovic N (2004) CarboPacTM PA20: a new monosaccharide separator column with electrochemical detection with disposable gold electrodes. J Biochem Biophys Methods 60:309–317.  https://doi.org/10.1016/j.jbbm.2004.01.009 CrossRefGoogle Scholar
  36. 36.
    Saeman JF, Moore WE, Mitchell RL, Millett MA (1954) Techniques for the determination of pulp constituents by quantitative paper chromatography. TAPPI J 37:336–343Google Scholar
  37. 37.
    Rahman S (2016) Size and concentration analysis of gold nanoparticles with ultraviolet-visible spectroscopy. Undergrad J Math Model One + Two 7:13.  https://doi.org/10.5038/2326-3652.7.1.4872 Google Scholar
  38. 38.
    Scherrer P (1918) Bestimmung der Größe und der inneren Struktur von Kolloidteilchen mittels Röntgenstrahlen. Nachrichten von der Gesellschaft der Wissenschaften zu Göttingen, Math Klasse 1918:98–100Google Scholar
  39. 39.
    Repetto G, del Peso A, Zurita JL (2008) Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nat Protoc 3:1125–1131.  https://doi.org/10.1038/nprot.2008.75 CrossRefGoogle Scholar
  40. 40.
    Trigui F, Pigeon P, Jalleli K et al (2013) Selection of a suitable disc bioassay for the screening of anti-tumor molecules. Int J Biomed Sci 9:230–236Google Scholar
  41. 41.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685CrossRefGoogle Scholar
  42. 42.
    Parker AR, Jolles S, Ponsford M et al (2018) Quantification of human C1 esterase inhibitor protein using an automated turbidimetric immunoassay. J Clin Lab Anal 33:e22627.  https://doi.org/10.1002/jcla.22627 Google Scholar
  43. 43.
    Saghir S, Iqbal MS, Hussain MA, Koschella A, Heinze T (2008) Structure characterization and carboxymethylation of arabinoxylan isolated from Ispaghula (Plantago ovata) seed husk. Carbohydr Polym 74:309–317.  https://doi.org/10.1016/j.carbpol.2008.02.019 CrossRefGoogle Scholar
  44. 44.
    Izydorczyk MS, Biliaderis CG, Lazaridou A et al (2007) Functional food carbohydrates. CRC Press, Boca RatonGoogle Scholar
  45. 45.
    Iram F, Massey S, Iqbal MS, Ward DG (2018) Structural investigation of hemicelluloses from Plantago ovata, Mimosa pudica and Lallemantia royleana by MALDI-ToF mass spectrometry. J Carbohydr Chem 37:1–17.  https://doi.org/10.1080/07328303.2018.1487973 CrossRefGoogle Scholar
  46. 46.
    Tomaszewska E, Soliwoda K, Kadziola K, et al (2013) Detection limits of DLS and UV-Vis spectroscopy in characterization of polydisperse nanoparticles colloids. J Nanomater 2013:60.  https://doi.org/10.1155/2013/313081
  47. 47.
    Bhattacharjee S (2016) DLS and zeta potential - what they are and what they are not? J Control Release 235:337–351CrossRefGoogle Scholar
  48. 48.
    Smith EF (1916) Studies on the crown gall of plants its relation to human cancer. J Cancer Res 1:231–309.  https://doi.org/10.1158/jcr.1916.231 Google Scholar
  49. 49.
    Kattumuri V, Katti K, Bhaskaran S, Boote EJ, Casteel SW, Fent GM, Robertson DJ, Chandrasekhar M, Kannan R, Katti KV (2007) Gum arabic as a phytochemical construct for the stabilization of gold nanoparticles: in vivo pharmacokinetics and X-ray-contrast-imaging studies. Small 3:333–341.  https://doi.org/10.1002/smll.200600427 CrossRefGoogle Scholar
  50. 50.
    Brown DH, McKinlay GC, Smith WE (1979) The electronic spectra of some gold(III) complexes. Inorg Chim Acta 32:117–121.  https://doi.org/10.1016/S0020-1693(00)91648-7 CrossRefGoogle Scholar
  51. 51.
    Swarbrick J (1996) Encyclopedia of pharmaceutical technology. Pharm Technol 3:2004–2020.  https://doi.org/10.1081/E-EPT-100001065 Google Scholar
  52. 52.
    Philip A, Philip B (2010) Colon targeted drug delivery systems: a review on primary and novel approaches. Oman Med J 25:70–78.  https://doi.org/10.5001/omj.2010.24 CrossRefGoogle Scholar
  53. 53.
    Iqbal MS, Taqi SG, Arif M, Wasim M, Sher M (2009) In vitro distribution of gold in serum proteins after incubation of sodium aurothiomalate and auranofin with human blood and its pharmacological significance. Biol Trace Elem Res 130:204–209.  https://doi.org/10.1007/s12011-009-8330-0 CrossRefGoogle Scholar
  54. 54.
    Israel L, Edelstein R, Mannoni P, Radot E, Greenspan EM (1977) Plasmapheresis in patients with disseminated cancer: clinical results and correlation with changes in serum protein. The concept of “nonspecific blocking factors.”. Cancer 40:3146–3154.  https://doi.org/10.1002/1097-0142(197712)40:6<3146::AID-CNCR2820400659>3.0.CO;2-N CrossRefGoogle Scholar
  55. 55.
    Robyt JF (1998) Essentials of carbohydrate chemistry. Springer Science & Business Media, BerlinCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019
corrected publication 2019

Authors and Affiliations

  1. 1.Department of ChemistryLCW UniversityLahorePakistan
  2. 2.Department of ChemistryForman Christian CollegeLahorePakistan
  3. 3.Radiopharmacy & PET Radiochemistry DivisionInstitute of Nuclear Medicine and OncologyLahorePakistan
  4. 4.Institute of Nuclear Medicine Oncology and RadiotherapyAbbottabadPakistan
  5. 5.Isotope Production DivisionPakistan Institute of Nuclear Science and Technology PO NiloreIslamabadPakistan
  6. 6.School of Biological SciencesUniversity of the Punjab, Quaid-e-Azam CampusLahorePakistan

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