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Use of 2-Hydrazinobenzothiazole-Modified Copolymer(s) as Potential Chelating Agent for Sensitive and Selective Determination of Low Levels of Mercury in Seafood by Ultrasound-Assisted Cloud-Point Extraction Combined with Spectrophotometry

  • H. B. Zengin
  • R. Gürkan
Article
  • 26 Downloads

Abstract

In this study, a new method was developed for the pre-concentration of trace mercury from seafood samples prior to analysis by spectrophotometry. The method is based on the complexation between 2-hydrazinobenzothiazole (2-HBT)-modified copolymer(s) and Hg(II) in the presence of an ionic surfactant, cetyltrimethylammonium bromide (CTAB), as sensitivity enhancer at pH 4.5 and the extraction of the complex into the surfactant-rich phase of polyethylene glycol tert-octylphenyl ether (Triton X-114) as the extractant. The variables affecting extraction efficiency were evaluated and optimized. Due to the observation that the modified copolymers are 2.5-fold more sensitive and selective to the Hg2+ ions than the CH3Hg+, the amounts of free Hg2+ and total Hg were determined at 325 nm by spectrophotometric detection of free Hg2+ and total Hg in the pre-treated and extracted fish samples using dilute acid mixture containing Triton X-114 and K2Cr2O7, before and after oxidation of CH3Hg+ to Hg2+ with mixture of KBr and KBrO3 in the acidic media. The amount of CH3Hg+ was calculated from the difference between total Hg and free Hg2+ amounts. The accuracy was tested by analysis of two certified samples. The results were statistically in good agreement with the certified values, and the precision was lower than 6.4%. The limits of detection were 1.40 (1.58) and 1.91 (2.11) μg L−1 for Hg2+ from the two calibration solutions spiked before the pre-treatment, respectively. It has been observed that there is no significant matrix effect by comparison of slopes of the calibration curves. The method was applied to seafood samples for speciation analysis of free Hg2+ and CH3Hg+. In terms of speciation, while total Hg is detected in the range of 12.6–143.8 μg kg−1, the distribution of mercury in seafood was in the range of 7.4–53.3 μg kg−1 for CH3Hg+ and in 8.3–90.5 μg kg−1 for free Hg2+.

Keywords

Hg2+ CH3Hg+ 2-Hydrazinobenzothiazole Copolymer Seafood Spectrophotometry Ultrasound-assisted cloud-point extraction 

Notes

Acknowledgements

The financial support from the Scientific Research Projects of the Commission, CUBAP, University of Cumhuriyet (Sivas, Turkey), is gratefully acknowledged. For the thermal and spectroscopic characterization of the modified copolymers, we would like to thank the technical staff of the Cumhuriyet University and the advanced technological research and application center (CUTAM) for the technical assistance and support.

Compliance with Ethical Standards

The authors have no financial relationship with the organization that sponsored the research.

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethical Approval

This article does not contain any studies with human or animal subjects.

Informed Consent

On behalf of the other author, informed consent was obtained from each participant included in the study.

References

  1. 1.
    Sanchez Uria JE, Sanz-Medel A (1998) Inorganic and methylmercury speciation in environmental samples. Talanta 47:509–524CrossRefGoogle Scholar
  2. 2.
    Rajabi HR, Shamsipur M, Zahedi MM, Roushani M (2015) On-line flow injection solid phase extraction using imprinted polymeric nanobeads for the preconcentration and determination of mercury ions. Chem Eng J 259:330–337CrossRefGoogle Scholar
  3. 3.
    Krishna MVB, Karunasagar D (2015) Robust ultrasound assisted extraction approach using dilute TMAH solutions for the speciation of mercury in fish and plant materials by cold vapour atomic absorption spectrometry (CVAAS). Anal Methods 7:1997–2005CrossRefGoogle Scholar
  4. 4.
    Matusiewicz H, Stanisz E (2010) Evaluation of various sample pre-treatment methods for total and inorganic mercury determination in biological certified reference materials by CVAAS technique. Cent Eur J Chem 8(3):594–601Google Scholar
  5. 5.
    Stanisz E, Werner J, Matusiewicz H (2013) Mercury species determination by task specific ionic liquid-based ultrasound-assisted dispersive liquid–liquid microextraction combined with cold vapour generation atomic absorption spectrometry. Microchem J 110:28–35CrossRefGoogle Scholar
  6. 6.
    Pourreza N, Ghanemi K (2009) Determination of mercury in water and fish samples by cold vapor atomic absorption spectrometry after solid phase extraction on agar modified with 2-mercaptobenzimidazole. J Hazard Mater 161:982–987CrossRefGoogle Scholar
  7. 7.
    Shah AQ, Kazi TG, Baig JA, Afridi HI, Kandhro GA, Arain MB, Kolachi NF, Wadhwa SK (2010) Total mercury determination in different tissues of broiler chicken by using cloud point extraction and cold vapor atomic absorption spectrometry. Food Chem Toxicol 48:65–69CrossRefGoogle Scholar
  8. 8.
    Rio-Segade S, Bendicho C (1999) Ultrasound-assisted extraction for mercury speciation by the flow injection–cold vapor technique. J Anal At Spectrom 14:263–268CrossRefGoogle Scholar
  9. 9.
    Cava-Montesinos P, Dominguez-Vidal A, Ververa ML, Pastor A, de la Guardia M (2004) On-line speciation of mercury in fish by cold vapour-atomic fluorescence through ultrasound assisted extraction. J Anal At Spectrom 19:1386–1390CrossRefGoogle Scholar
  10. 10.
    Yu L-P (2005) Cloud point extraction preconcentration prior to high-performance liquid chromatography coupled with cold vapor generation atomic fluorescence spectrometry for speciation analysis of mercury in fish samples. J Agric Food Chem 53:9656–9662CrossRefGoogle Scholar
  11. 11.
    Aranda PR, Gil RA, Moyano S, De Vito IE, Martinez LD (2008) Cloud point extraction of mercury with PONPE 7.5 prior to its determination in biological samples by ETAAS. Talanta 75:307–311CrossRefGoogle Scholar
  12. 12.
    Locatelli C, Melucci D (2013) Voltammetric method for ultra-trace determination of total mercury and toxic metals in vegetables. Comparison with spectroscopy. Cent Eur J Chem 11(5):790–800Google Scholar
  13. 13.
    Doker S, Bosgelmez II (2015) Rapid extraction and reverse phase-liquid chromatographic separation of mercury(II) and methylmercury in fish samples with inductively coupled plasma mass spectrometric detection applying oxygen addition into plasma. Food Chem 184:147–153CrossRefGoogle Scholar
  14. 14.
    Li L, Wang Z, Zhang S, Wang M (2017) Directly-thiolated graphene based organic solvent-free cloud point extraction-like method for enrichment and speciation of mercury by HPLC-ICP-MS. Microchem J 132:299–307CrossRefGoogle Scholar
  15. 15.
    Altunay N (2018) Utility of ultrasound assisted-cloud point extraction and spectrophotometry as a preconcentration and determination tool for the sensitive quantification of mercury species in fish samples. Spectrochim Acta A 189:167–175CrossRefGoogle Scholar
  16. 16.
    Ulusoy HI, Gurkan R, Ulusoy S (2012) Cloud point extraction and spectrophotometric determination of mercury species at trace levels in environmental samples. Talanta 88:516–523CrossRefGoogle Scholar
  17. 17.
    Khammas ZA-A, Ghali AA (2013) Cloud point extraction procedure for the determination of mercury by spectrophotometry using a new synthesized ligand. Iraqi National J Chem 49:25–37Google Scholar
  18. 18.
    Dittert IM, Maranhão TA, Borges DL, Vieira MA, Welz B, Curtius AJ (2007) Determination of mercury in biological samples by cold vapor atomic absorption spectrometry following cloud point extraction with salt-induced phase separation. Talanta 72(5):1786–1790CrossRefGoogle Scholar
  19. 19.
    Depoi F dos S, Bentlin FRS, Pozebon D (2010) Methodology for Hg determination in honey using cloud point extraction and cold vapour-inductively coupled plasma optical emission spectrometry. Anal Methods 2:180–185CrossRefGoogle Scholar
  20. 20.
    Li Y, Hu B (2007) Sequential cloud point extraction for the speciation of mercury in seafood by inductively coupled plasma optical emission spectrometry. Spectrochim Acta B 62:1153–1160CrossRefGoogle Scholar
  21. 21.
    Gharehbaghi M, Shemirani F, Baghdadi M (2009) Dispersive liquid–liquid microextraction based on ionic liquid and spectrophotometric determination of mercury in water samples. Int J Environ Anal Chem 89(1):21–33CrossRefGoogle Scholar
  22. 22.
    Hossien ZM, Chamsaz M, Heidari T, Zavar MHA, Behbahani M, Salarian M (2014) Application of dispersive liquid–liquid micro-extraction using mean centering of ratio spectra method for trace determination of mercury in food and environmental samples. Food Anal Methods 7(2):352–359CrossRefGoogle Scholar
  23. 23.
    Shah AQ, Kazi TG, Baig JA, Afridi HI, Kandhro GA, Khan S, Kolachi NF, Wadhwa SK (2010) Determination of total mercury in muscle tissues of marine fish species by ultrasonic assisted extraction followed by cold vapor atomic absorption spectrometry. Pak J Anal Environ Chem 11(2):12–17Google Scholar
  24. 24.
    Eshaghi Z, Bardajee GR, Azimi S (2016) Magnetic dispersive micro solid-phase extraction for trace mercury pre-concentration and determination in water, hemodialysis solution and fish samples. Microchem J 127:170–177CrossRefGoogle Scholar
  25. 25.
    Najafi E, Aboufazeli F, Zhad HRLZ, Sadeghi O, Amani V (2013) A novel magnetic ion imprinted nano-polymer for selective separation and determination of low levels of mercury(II) ions in fish samples. Food Chem 141(4):4040–4045CrossRefGoogle Scholar
  26. 26.
    Pena-Pereira F, Lavilla I, Bendicho C, Vidal L, Canals A (2009) Speciation of mercury by ionic liquid-based single-drop microextraction combined with high-performance liquid chromatography-photodiode array detection. Talanta 78:537–541CrossRefGoogle Scholar
  27. 27.
    Zhua S, Chen B, He M, Huang T, Hu B (2017) Speciation of mercury in water and fish samples by HPLC-ICP-MS after magnetic solid phase extraction. Talanta 171:213–219CrossRefGoogle Scholar
  28. 28.
    Bezerra MDA, Arruda MAZ, Ferreira SLC (2005) Cloud point extraction as a procedure of separation and pre-concentration for metal determination using spectroanalytical techniques: a review. Appl Spectrosc Rev 40:269–299CrossRefGoogle Scholar
  29. 29.
    Carabias-Martínez R, Rodríguez-Gonzalo E, Domínguez-Alvarez J, Hernández-Méndez J (1999) Cloud point extraction as a preconcentration step prior to capillary electrophoresis. Anal Chem 71:2468–2474CrossRefGoogle Scholar
  30. 30.
    Paleologos EK, Giokas DL, Karayannis MI (2005) Micelle-mediated separation and cloud-point extraction. TrAC Trends Anal Chem 24:426–436CrossRefGoogle Scholar
  31. 31.
    Sikalos TI, Paleologos EK, Sikalos TI, Paleologos EK (2005) Cloud point extraction coupled with microwave or ultrasonic assisted back extraction as a preconcentration step prior to gas chromatography. Anal Chem 77(8):2544–2549CrossRefGoogle Scholar
  32. 32.
    Gürkan R, Altunay N (2015) Quantification of 5-hydroxymethylfurfural in honey samples and acidic beverages using spectrophotometry coupled with ultrasonic-assisted cloud point extraction. J Food Compos Anal 42:141–151CrossRefGoogle Scholar
  33. 33.
    Altunay N, Elik A, Gürkan R (2018) Extraction and reliable determination of acrylamide from thermally processed foods using ionic liquid-based ultrasound-assisted selective microextraction combined with spectrophotometry. Food Addit Contam A 35(2):222–232CrossRefGoogle Scholar
  34. 34.
    Temel NK, Gürkan R (2018) Using Safranin T as a charge transfer-sensitive ion-pairing reagent in ultrasound-assisted cloud point extraction: determination of bisphenol A in selected beverages. J AOAC Int 101(1):277–287CrossRefGoogle Scholar
  35. 35.
    Altunay N, Gürkan R, Kır U (2016) Spectrophotometric determination of low levels arsenic species in beverages after ion-pairing vortex-assisted cloud-point extraction with acridine red. Food Addit Contam A 33(2):259–270Google Scholar
  36. 36.
    Gürkan R, Kır U, Altunay N (2015) Development of a simple, sensitive and inexpensive ion-pairing cloud point extraction approach for the determination of trace inorganic arsenic species in spring water, beverage and rice samples by UV-Vis spectrophotometry. Food Chem 180:32–41CrossRefGoogle Scholar
  37. 37.
    Gürkan R, Kir U (2013) A fast and reliable method for quantitative determination of total mercury in vegetables. Toxicol Environ Chem 95(10):1659–1674CrossRefGoogle Scholar
  38. 38.
    Leardi R (2009) Experimental design in chemistry: a tutorial. Anal Chim Acta 652:161–172CrossRefGoogle Scholar
  39. 39.
    Olivieri AC (2015) Practical guidelines for reporting results in single- and multi-components analytical calibration: a tutorial. Anal Chim Acta 818:10–22CrossRefGoogle Scholar
  40. 40.
    Crea F, de Stefano C, Foti C, Milea D, Sammartano S (2014) Chelating agents for the sequestration of mercury(II) and monomethylmercury(II). Curr Med Chem 21:3819–3836CrossRefGoogle Scholar
  41. 41.
    Ojeda CB, Rojas FS (2009) Separation and preconcentration by a cloud point extraction procedure for determination of metals: an overview. Anal Bioanal Chem 394:759–782CrossRefGoogle Scholar
  42. 42.
    Tavallali H, Asrari E, Attaran AM, Tabandeh M (2010) Sensitive determination of lead in soil and water samples by cloud point extraction-flame atomic absorption spectrometry method. Int J Chem Tech Res 2:1731–1173Google Scholar
  43. 43.
    Santaladchaiyakit Y, Srijaranai S (2012) A simplified ultrasound-assisted cloud-point extraction method coupled with high performance liquid chromatography for residue analysis of benzimidazole anthelmintics in water and milk samples. Anal Methods 4:3864–3873CrossRefGoogle Scholar
  44. 44.
    Shokrollahi A, Ghaedi M, Hossaini O, Khanjari N, Soylak M (2008) Cloud point extraction and flame atomic absorption spectrometry combination for copper(II) ion in environmental and biological samples. J Hazard Mater 160:435–440CrossRefGoogle Scholar
  45. 45.
    Gu T, Galera-Gomez PA (1995) Clouding of Triton X-114: the effect of added electrolytes on the cloud point of Triton X-114 in the presence of ionic surfactants. Colloids Surf A 104:307–312CrossRefGoogle Scholar
  46. 46.
    Jia G, Lv C, Zhu W, Qiu J, Wang X, Zhou Z (2008) Applicability of cloud point extraction coupled with microwave-assisted back-extraction to the determination of organophosphorous pesticides in human urine by gas chromatography with flame photometry detection. J Hazard Mater 159:300–305CrossRefGoogle Scholar
  47. 47.
    Pourreza N, Rastegarzadeh S, Larki A (2008) Micelle-mediated cloud point extraction and spectrophotometric determination of rhodamine B using Triton X-100. Talanta 77:733–736CrossRefGoogle Scholar
  48. 48.
    Pearson RG (1963) Hard and soft acids and bases. J Am Chem Soc 85:3533–3539CrossRefGoogle Scholar
  49. 49.
    (2008) Commission Regulation (EC) No. 629/2008 of 2 July amending Regulation (EC) No. 1881/2006 setting maximum levels for certain contaminants in foodstuffs. Off J Eur Union 173:6–9Google Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Chemistry, Faculty of SciencesCumhuriyet UniversitySivasTurkey

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