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Selenium-Yeast Alleviated Inflammatory Damage Caused by Lead via Inhibiting Ras/ERK Pathway and Inflammatory Factors in Chicken Skeletal Muscles

  • Zhe Liu
  • Feng Zhang
  • Ping Lu
  • Rui Zhao
  • Hua Zhang
  • Baifen Song
  • Liyang Li
  • Zhijun Wu
  • Rui WuEmail author
Article
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Abstract

The aim of this study was to investigate the ameliorative effects of selenium-enriched yeast (Se-yeast) on the inflammatory damage induced by lead (Pb) in chicken skeletal muscles. A total of 108 1-day-old broiler chickens were randomly allocated into four groups (n = 27/group): the control group (C group), the Se-yeast-supplemented group (Se group), the lead-treated group (Pb group), and finally the Se- and Pb-combined group (Pb/Se group). The C group was fed with a basic diet comprising 0.049 mg/kg Se and 0.1 mg/kg Pb while the Se group was fed a Se-yeast diet containing 0.30 mg/kg Se and 0.1 mg/kg Pb. Similarly, the Pb group was fed a Pb acetate diet containing 0.049 mg/kg Se and 350 mg/kg Pb while the Pb/Se group was fed with a Se-yeast diet containing 0.30 mg/kg Se and 350 mg/kg Pb. On days 7, 21, and 35 after commencing the experiment, nine chicks belonging to each group were euthanized and the samples were analyzed by employing the techniques of inductively coupled plasma mass spectrometry and real-time quantitative PCR, along with Western blotting. The results indicated that excess Pb increased the nitric oxide concentration, enhanced the activity of inducible nitric oxide synthase (iNOS), and the mRNA levels of interleukin 1β (IL-1β), interleukin 4 (IL-4), interleukin 10 (IL-10), and interferon gamma (IFN-γ) in a time-dependent manner. Further, it was found that Se reduced damage caused by Pb by decreasing the expression of inflammatory factors in chicken skeletal muscles. Taken together, the results from this study provide the theoretical basis for an alleviate effect of Se on Pb-induced inflammatory damage in chicken skeletal muscles, mediated by inhibiting the Ras/extracellular signal-regulated kinase (ERK) pathway and the inflammatory factors.

Keywords

Lead Selenium-yeast Skeletal muscles Inflammatory damage Signal-regulated 

Notes

Acknowledgments

The authors wish to thank the study participants and Jilin University for their contributions to this study. All of the authors have read the manuscript and have agreed to submit it, in its current form, for consideration for publication.

Funding Information

This work was supported by grants from the Foundation Project of Heilongjiang province natural (No. H2017038), National key research and development program of the 13th five-year plan(2016YFD0501203), Heilongjiang University Students Innovation and Entrepreneurship Training Project (201810223079), and the Startup Foundation for the Doctors in Heilongjiang Bayi Agricultural University (Grant No. XDB-2017-18), and the School Cultivation Project Foundation in Heilongjiang Bayi Agricultural University (XZR2016-12).

Compliance with Ethical Standards

All experiments were approved by the Institutional Animal Care and Use Committee (Permission number: 22-05-01).

Conflict of Interest

The authors declare that they have no competing interests.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Colloge of Life Sciences and BiotechnologyHeilongjiang Bayi Agricultural UniversityDaqingPeople’s Republic of China
  2. 2.Department of OsteologyThe Daqing Oil Field General HospitalDaqingPeople’s Republic of China
  3. 3.China Animal Health And Epidemiology CenterQingdaoPeople’s Republic of China
  4. 4.College of Animal Science and Veterinary MedicineHeilongjiang Bayi Agricultural UniversityDaqingPeople’s Republic of China

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