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Biological Trace Element Research

, Volume 189, Issue 2, pp 490–500 | Cite as

Protective Role of Selenium Against Hemolytic Anemia Is Mediated Through Redox Modulation

  • Rankaljeet Kaur
  • Preety Ghanghas
  • Pulkit Rastogi
  • Naveen KaushalEmail author
Article

Abstract

Selenium (Se), an essential trace element and potent nutritional antioxidant, exerts its biological effects through incorporation into selenoproteins like glutathione peroxidase (GPx). Modest decrement in the levels of GPx could be partly responsible for peroxidation of RBCs, which results into hemolytic anemia. Therefore, it is hypothesized that dietary Se, as selenoproteins (GPx), can maintain the homeostasis in RBCs and regulate the erythropoiesis by preventing oxidative stress-mediated hemolysis. Se-deficient (0.01 ppm), Se-adequate (0.1 ppm sodium selenite), and Se-supplemented (0.5 ppm sodium selenite) status were created in Balb/c mice by feeding yeast-based diets for 8 weeks and established by measuring Se levels in plasma and activities, expressions of Se-dependent selenoproteins. Fifty percent of mice from each differential Se group were treated with phenylhydrazine (PHZ, 20 mg/kg, i.p.) to induce hemolytic anemia. Results indicated that PHZ-treated Se-deficient animals demonstrated increased hemolysis, abnormal RBC morphology, increase in Heinz bodies and reticulocytes, and denaturation of hemoglobin to globin precipitates and methemoglobin. Se supplementation protected against these hemolytic changes and makes RBCs less fragile. These findings were consistent with dietary Se concentration-dependent changes in activity and expression of GPx indicating that ROS-mediated oxidative stress is integral to hemolysis. Protective effects of Se supplementation against increased levels of ROS, protein carbonyls, and peroxide damage to membrane lipids and enzymatic antioxidants validated these observations. In conclusion, dietary Se supplementation protected the RBCs against hemolysis by mitigating ROS-mediated oxidative stress.

Keywords

Selenium Hemolytic anemia Oxidative stress Phenylhydrazine GPx 

Notes

Funding

Research reported in this publication was supported by the UGC-SAP (F.4-1/2015/DSA-1 (Sap-II)) and DST-FIST (SR/FST/LS1-645) programs sanctioned to the Department of Biophysics, Panjab University, Chandigarh (160014), India, by the University Grants Commission (UGC), Govt of India and the Department of Science and Technology (DST), Govt of India, respectively. The financial assistance to Panjab University by DST through DST-PURSE program is also duly acknowledged.

Compliance with Ethical Standards

All the experiments were performed in accordance with the guidelines of institutional ethical committee of Panjab University, Chandigarh.

Conflict Interest

The authors declare that they have no conflict of interest.

Supplementary material

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Rankaljeet Kaur
    • 1
  • Preety Ghanghas
    • 1
  • Pulkit Rastogi
    • 2
  • Naveen Kaushal
    • 1
    Email author
  1. 1.Department of BiophysicsPanjab UniversityChandigarhIndia
  2. 2.Department of HematologyPostgraduate Institute of Medical Education and Research, (PGIMER)ChandigarhIndia

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