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Biological Trace Element Research

, Volume 187, Issue 2, pp 367–375 | Cite as

The Importance of Se-Related Genes in the Chondrocyte of Kashin–Beck Disease Revealed by Whole Genomic Microarray and Network Analysis

  • Sen Wang
  • Guanghui Zhao
  • Wanzhen Shao
  • Huan Liu
  • Weizhuo Wang
  • Cuiyan Wu
  • Mikko J. Lammi
  • Xiong Guo
Article
  • 115 Downloads

Abstract

Kashin–Beck disease (KBD) is an endemic, chronic, and degenerative osteoarthropathy. Selenium (Se) deficiency plays important role in the pathogenesis of KBD. We aimed to screen Se-related gene from chondrocytes of patients with KBD. Whole-genome oligonucleotide microarrays were used to detect differentially expressed genes. qRT-PCR was used to confirm the microarray results. Comparative Toxicogenomics Database (CTD) was used to screen Se-related genes from differentially expressed genes. Gene Ontology (GO) classifications and network analysis of Se-related genes were constituted by STRING online system. Three hundred ninety-nine differentially expressed genes were obtained from microarray. Among them, 54 Se-related genes were identified by CTD. The qRT-PCR validation showed that four genes expressed similarly with the ones in the microarray transcriptional profiles. The Se-related genes were categorized into 6 cellular components, 8 molecular functions, 44 biological processes, 10 pathways, and 1 network by STRING. The Se-related gene insulin-like growth factor binding protein 2 (IGFBP2), insulin-like growth factor binding protein 3 (IGFBP3), interleukin 6 (IL6), BCL2, apoptosis regulator (BCL2), and BCL2-associated X, apoptosis regulator (BAX), which involved in many molecular functions, biological processes, and apoptosis pathway may play important roles in the pathogenesis of KBD.

Keywords

Trace element Selenium Kashin–Beck disease Chondrocyte Microarray STRING 

Notes

Acknowledgments

This study was supported by grants from the National Natural Science Foundation of China (81402638, 81472924, and 81502766) and Key International Cooperation Projects from National Natural Science Foundation of China (81620108026).

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.

Supplementary material

12011_2018_1404_Fig2_ESM.png (1.5 mb)
Fig.S1

The mRNA levels for IGFBP2, IL6, APOL3 and FBLN1 in chondrocytes of KBD patients and controls. The lines inside the boxes denote the medians. The boxes mark the interval between the 25 and 75 percentiles. The whiskers denote the interval between the 10 and 90 percentiles (PNG 1556 kb)

12011_2018_1404_MOESM1_ESM.tif (503 kb)
High resolution image (TIF 503 kb)
12011_2018_1404_MOESM2_ESM.docx (48 kb)
ESM 2 (DOCX 48 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Sen Wang
    • 1
    • 2
  • Guanghui Zhao
    • 3
  • Wanzhen Shao
    • 1
    • 2
  • Huan Liu
    • 1
    • 2
  • Weizhuo Wang
    • 4
  • Cuiyan Wu
    • 1
    • 2
  • Mikko J. Lammi
    • 1
    • 2
    • 5
    • 6
  • Xiong Guo
    • 1
    • 2
    • 6
  1. 1.School of Public HealthHealth Science Center of Xi’an Jiaotong UniversityXi’anChina
  2. 2.Key Laboratory of Trace Elements and Endemic DiseasesNational Health and Family Planning CommissionXi’anChina
  3. 3.Xi’an Honghui HospitalHealth Science Center of Xi’an Jiaotong UniversityXi’anChina
  4. 4.Orthopedic Departmentthe Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’anChina
  5. 5.Department of Integrative Medical BiologyUniversity of UmeåUmeåSweden
  6. 6.Xi’anChina

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