Biological Trace Element Research

, Volume 187, Issue 1, pp 181–191 | Cite as

Moringa oleifera Leaves Extract Protects Titanium Dioxide Nanoparticles-Induced Nephrotoxicity via Nrf2/HO-1 Signaling and Amelioration of Oxidative Stress

  • K. H. Abdou
  • Walaa A. MoselhyEmail author
  • Hanaa M. Mohamed
  • El-Shaymaa El-Nahass
  • Ahlam G. Khalifa


The efficacy of Moringa oleifera leaf extract (MO) in alleviating nephrotoxicity induced by titanium dioxide nanoparticles (TiO2 NPs) was studied. Rats were divided into four groups. Group I received distilled water. Group II received TiO2NPs. Group III received both TiO2NPs suspension beside MO. Group IV received MO only. Kidney KIM-1, NF-кB TNF-α, and HSP-70 expression were significantly upregulated while both Nrf2 and HO-1were significantly downregulated in TiO2NPs-treated rats. MO decreases expression of KIM-1, NF-кB, TNF-α, and HSP-70. In addition, MO has markedly upregulated the expression of Nrf2 and HO-1. In conclusion, MO can inhibit nephrotoxicity by suppressing oxidative stress and inflammation. These effects are suggested to be mediated by activating Nrf2/HO-1.The biochemical analysis and histopathological finding reinforced these results. These data support the antioxidant properties’ nutraceutical role of MO against TiO2NPs-induced toxicity.


Titanium dioxide nanoparticles Moringa oleifera Nephrotoxicity Nrf2/HO-1 Oxidative stress 


Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflicts of interest.


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Authors and Affiliations

  1. 1.Department of Toxicology and Forensic Medicine, Faculty of Veterinary MedicineBeni-Suef UniversityBeni-SuefEgypt
  2. 2.Genetic & Molecular Biology, Zoology Department, Faculty of ScienceBeni-Suef UniversityBeni-SuefEgypt
  3. 3.Department of Pathology, Faculty of Veterinary MedicineBeni-Suef UniversityBeni-SuefEgypt

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