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Biological Trace Element Research

, Volume 186, Issue 1, pp 267–278 | Cite as

Effect of Dietary Zinc-Nanoparticles on Growth Performance, Anti-Oxidative and Immunological Status of Fish Reared Under Multiple Stressors

  • Neeraj Kumar
  • Kishore Kumar Krishnani
  • Narendra Pratap Singh
Article

Abstract

Zinc is one of the essential micronutrients that can be obtained via water and diet in aquatic animals to meet their physiological needs. The present study was designed to understand the effect of the supplementation of zinc nanoparticles (Zn-NPs) in mitigating abiotic and biotic stress in Pangasius hypophthalmus. Two zinc nanoparticle-incorporated diets with 10 and 20 mg/kg nanoparticles and a control without zinc nanoparticles were formulated. To study the effect of formulated feeds on stress tolerance, fish were exposed to sublethal dose (4 ppm) of Pb (lead) and temperature at 34 °C. Two hundred and seventy-three fish were randomly distributed into seven treatment groups in triplicates, namely a control group (no Zn-NPs and no Pb and temperature exposure, Ctr/Ctr), control diet fed and exposed to Pb (Ctr/Pb), control diet fed and concurrently exposed to Pb and temperature (Pb-T/Ctr), and Zn-NPs 10 and 20 mg/kg diet with or without stressors (Zn-NPs 10 mg/kg, Zn-NPs 20 mg/kg, Pb-T/Zn-NPs 10 mg/kg, Pb-T/Zn-NPs 20 mg/kg). The effect of Zn-NPs on growth performance, stress biomarkers, biochemical and immunological responses, and survival of P. hypophthalmus following challenge with pathogenic bacteria were evaluated. The growth performance was noticeably (p < 0.01) enhanced, and anti-oxidative stress (catalase, superoxide dismutase, and glutathione-s-transferase) significantly reduced in the Zn-NPs supplemented groups. Similarly, immunological parameters such as total protein, albumin, globulin, and A/G ratio significantly improved, and stress biomarkers such as blood glucose, cortisol, and HSP 70 were reduced in Zn-NPs supplemented groups. Overall, the results suggest that supplementation of dietary Zn-NPs with less concentration in the diet has a definitive role in the mitigation of abiotic and biotic stress in P. hypophthalmus.

Keywords

Zinc nanoparticles Growth performance Stress biomarkers Immunity Lead Pangasius hypophthalmus 

Notes

Acknowledgements

The authors express sincere gratitude to the Director, ICAR-National Institute of Abiotic Stress Management, Baramati, Pune, for providing all the facilities to conduct the present work. The financial assistance has been provided by Indian Council of Agricultural Research (ICAR), New Delhi, India, as an institutional project (no. IXX09673) is gratefully acknowledged. Authors are grateful to Dr. Veera M. Boddu, Research Leader, National Centre for Agriculture Utilization Research (NCAUR) Agricultural Research Service, US Department of Agriculture, ARS/USDA USA, Dr. Sanath H Kumar, Senior Scientist, Central Institute of Fisheries Education, Mumbai India, and Dr. Sib Sankar Giri, Laboratory of Aquatic Biomedicine, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul, South Korea, for English editing and correction. I also place a record of thanks to Mrs. Yogita and Mr. Yuvraj Sanas for their technical assistance.

Compliance with Ethical Standards

Conflict of Interest

All authors declare that they have no conflict of interest.

Supplementary material

12011_2018_1285_MOESM1_ESM.docx (7.9 mb)
ESM 1 (DOCX 847 kb)

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Authors and Affiliations

  1. 1.ICAR-National Institute of Abiotic Stress Management (NIASM)PuneIndia

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