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Biological Trace Element Research

, Volume 185, Issue 1, pp 162–169 | Cite as

The Antagonistic Effect of Selenium on Lead-Induced Immune Dysfunction via Recovery of Cytokine and Heat Shock Protein Expression in Chicken Neutrophils

  • Mengyuan Xing
  • Xi Jin
  • Jinliang Wang
  • Qunxiang Shi
  • Jingzeng Cai
  • Shiwen Xu
Article

Abstract

Lead (Pb) is a ubiquitous and toxic heavy metal and it can damage the immune system in humans and animals. Many researchers have reported that Selenium (Se) could possess various pharmacological effects in mammals. However, few studies have been carried out to investigate the protective role of Se in birds, especially in chickens. In this study, we investigated the protective effects of Se against Pb-induced inflammatory responses and the expression of heat shock proteins (HSPs) in peripheral blood neutrophils. One hundred eighty Hy-Line brown chickens were randomly divided into the control group (Con group), Se supplementation group (+Se group), Pb supplementation group (+Pb group), and the Se and Pb compound group (Se+Pb group). On the 90th day of the experiment, the peripheral blood was collected to extract neutrophils, and then, the levels of HSPs and cytokines were examined. The results showed that, after Pb treatment, the levels of IL-(1β, 1R, 4, 8, 10, and 12β), TGF-β4, and HSP (27, 40, 60, 70, and 90) mRNA were significantly increased and levels of IL-2 and IFN-γ mRNA were decreased compared with those in the control group. Compared with the control group, the protein levels of HSP60 and HSP70 were also increased in the Pb treatment group. Co-administration of Se (1 mg/kg/day) and Pb resulted in a reversal of the Pb-induced cytokine changes in neutrophils accompanied by a significant decrease in HSPs. Our study demonstrated that Pb could decrease the immune function via changing the expression of cytokines and HSPs in chicken neutrophils, but Se could relieve the toxic effect induced by Pb.

Keywords

Lead Selenium Neutrophil Chickens Cytokines Heat shock proteins 

Notes

Funding Information

This study was supported by the National Natural Science Foundation of China (31272626) and the International (Regional) Cooperation and Exchange Projects of the National Natural Science Foundation of China (31320103920).

Compliance with Ethical Standards

All procedures used in this study were approved by the Institutional Animal Care and Use Committee of Northeast Agricultural University.

Conflict of Interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2017

Authors and Affiliations

  • Mengyuan Xing
    • 1
  • Xi Jin
    • 1
  • Jinliang Wang
    • 2
  • Qunxiang Shi
    • 1
  • Jingzeng Cai
    • 1
  • Shiwen Xu
    • 1
  1. 1.College of Veterinary MedicineNortheast Agricultural UniversityHarbinPeople’s Republic of China
  2. 2.Shandong Binzhou Animal Science & Veterinary Medicine AcademyBinzhouPeople’s Republic of China

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