Biological Trace Element Research

, Volume 184, Issue 1, pp 259–267 | Cite as

Effects of Zinc Oxide Nanoparticles on Performance, Egg Quality, Tissue Zinc Content, Bone Parameters, and Antioxidative Status in Laying Hens

  • Mehdi Abedini
  • Farid Shariatmadari
  • Mohammad Amir Karimi Torshizi
  • Hamed Ahmadi


The objective of this study was to observe the effects of dietary supplementation with zinc oxide nanoparticles (ZnO-NPs) on performance, egg quality, tissue Zn content, bone parameters, superoxide dismutase activity (SOD), and egg malondialdehyde (MDA) content in laying hens. A total of 288 laying hens at 64 weeks of age were randomly assigned to 4 treatments, 6 replicates, with 12 birds each. Experimental diets included the based diet (without Zn supplementation), and basal diet supplemented with 40, 80, and 120 mg Zn/kg from ZnO-NPs. Feed intake and egg mass were significantly higher in the 40 and 80 ZnO-NPs groups than the other groups. The birds in the 80 ZnO-NPs group had significantly higher egg product than the 120 ZnO-NPs and control groups. Egg shell thickness and shell strength significantly increased in the 40 and 80 ZnO-NPs groups. Moreover, Haugh unit significantly improved in the groups supplemented with ZnO-NPs, compared to the control group. Bone-breaking strength was significantly greater in the 80 ZnO-NPs group than the 120 ZnO-NPs and control groups. Also, ash weight was significantly greater in the 40 and 80 ZnO-NPs groups than the control group. There were significant differences among the groups in the Zn content in plasma, tibia, liver, pancreas, and egg. Relative to the control group, ZnO-NPs supplementation significantly increased the activities of SOD in the liver, pancreas, and plasma. The MDA content in egg was significantly reduced in the groups supplemented with ZnO-NPs. In conclusion, this study demonstrates that ZnO-NPs as dietary supplementation can improve the performance of laying hens, and levels of 40 to 80 ZnO-NPs are the optimal concentrations.


Laying hens Performance Eggshell quality Bone parameters Enzyme activities Nanoparticles 


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Mehdi Abedini
    • 1
  • Farid Shariatmadari
    • 1
  • Mohammad Amir Karimi Torshizi
    • 1
  • Hamed Ahmadi
    • 1
  1. 1.Department of Poultry Science, Faculty of AgricultureTarbiat Modares UniversityTehranIran

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