Biological Trace Element Research

, Volume 183, Issue 1, pp 80–91 | Cite as

Alleviation of Arsenic-Induced Pulmonary Oxidative Damage by GSPE as Shown during In vivo and In vitro Experiments

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Abstract

A long-term exposure to arsenic may lead to lung damage due to oxidative stress. In this context, GSPE can play a major role as a strong antioxidant. Our study attempted to reveal the connection between arsenic-induced lung injury and the antagonistic effect of GSPE. For this purpose, BEAS-2B cells and Kunming mice were exposed to different dosages of As2O3 and GSPE. Oxidative stress indicators were detected both in vivo and in vitro. Cell survival rate and morphological changes in the lung tissue (H&E staining) were evaluated as well. It was exhibited that As2O3 increased oxidative stress both in vivo and in vitro and decreased cells viability. In contrast, higher cell survival rate was revealed in the group treated with arsenic plus GSPE after 24 h as compared to that in the arsenic group. GSPE effectively reduced oxidative stress levels, along with increasing antioxidant capacity. In vivo experiments in arsenic-exposed group showed alveolar septum to be significantly thickened with considerable capillary congestion and invasion by inflammatory cells. After the intervention with GSPE, there seemed to be a dramatic reversal of morphology with thinning of the alveolar septum, decrease in capillary congestion, and number of inflammatory cells. This had shown that GSPE can effectively reduce the levels of oxidative stress, induced by arsenic in mice lung tissue. Conversely, antioxidant enzymes or products were increased. The experiment proved that GSPE can protect the lungs from oxidative damage induced by arsenic, and it may also be used as an antagonist against arsenic injuries.

Keywords

Arsenic Oxidative damage GSPE Mice lung tissues 

Notes

Acknowledgements

The authors would like to thank the Department of Public Health, Shihezi University School of Medicine for assistance with this work, as well as funding from National Natural Science Foundation of China (No. 81560517), the Key Areas of Science and Technology Research Project of Xinjiang Production and Construction Corps (No. 2015AG014, No.2014BA039) and the International Cooperative Project of Shihezi University (No. GJHZ201602).

Author’s Contribution

Experiments conceived and designed by SL MW FG and performed by MW, FG, DR, HW, GF, SL, GS. Analyses of the data are performed by SL, DR. Reagents, materials, and analysis tools were contributed by MW, GS, SL, and FG. The manuscript was written by SL, MW, and FG and revised by SL and GS.

Compliance with Ethical Standards

The present research was approved by the Ethical Committee of the First Affiliated Hospital of School of Medicine, Shihezi University (Approval ID: 2015-076-01). In this study, we were permitted to collect the blood samples and lung tissues of mice to detect the biochemical parameters.

Conflict of Interests

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Shihezi University School of MedicineShiheziChina

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