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Biological Trace Element Research

, Volume 178, Issue 2, pp 218–227 | Cite as

Oxidative Stress and Genotoxicity of Zinc Oxide Nanoparticles to Pseudomonas Species, Human Promyelocytic Leukemic (HL-60), and Blood Cells

  • Deepika Soni
  • Deepa Gandhi
  • Prashant Tarale
  • Amit Bafana
  • R.A. Pandey
  • Saravanadevi SivanesanEmail author
Article

Abstract

In the present study, toxicity of commercial zinc oxide nanoparticles (ZnO NPs) was studied on the bacterium Pseudomonas sp., human promyelocytic leukemia (HL-60) cells, and peripheral blood mononuclear cells (PBMC). The toxicity was assessed by measuring growth, cell viability, and protein expression in bacterial cell. The bacterial growth and viability decreased with increasing concentrations of ZnO NP. Three major proteins, ribosomal protein L1 and L9 along with alkyl hydroperoxides reductase, were upregulated by 1.5-, 1.7-, and 2.0-fold, respectively, after ZnO NP exposure. The results indicated oxidative stress as the leading cause of toxic effect in bacteria. In HL-60 cells, cytotoxic and genotoxic effects along with antioxidant enzyme activity and reactive oxygen species (ROS) generation were studied upon ZnO NP treatment. ZnO NP exhibited dose-dependent increase in cell death after 24-h exposure. The DNA-damaging potential of ZnO NP in HL-60 cells was maximum at 0.05 mg/L concentration. Comet assay showed 70–80% increase in tail DNA at 0.025 to 0.05 mg/L ZnO NP concentration. A significant increase of 1.6-, 1.4-, and 2.0-fold in ROS level was observed after 12 h. Genotoxic potential of ZnO NPs was also demonstrated in PBMC through DNA fragmentation. Thus, ZnO NP, besides being an essential element having antibacterial activity, also showed toxicity towards human cells (HL-60 and PBMC).

Keywords

ZnO NP Cytotoxicity MTT Protein expression ROS DNA damage 

Notes

Acknowledgements

The authors are thankful to CSIR-NEERI for providing necessary facilities to conduct the research work. Deepika Soni is grateful to the Department of Science and Technology (DST), Government of India, for the award of INSPIRE fellowship (IF10154). This manuscript represents CSIR-NEERI communication number KRC/2016/JUN/EBD-EHD/1.

Compliance with Ethical Standards

Peripheral blood mononuclear cells (PBMC) were obtained from healthy human donor (nonsmoker, nonalcoholic, and under no medication) following the ethical approval from the research ethics committee and standards laid down in 1964 with latest amendments of Declaration of Helsinki [20].

Conflict of Interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Deepika Soni
    • 1
  • Deepa Gandhi
    • 2
  • Prashant Tarale
    • 2
  • Amit Bafana
    • 2
  • R.A. Pandey
    • 1
  • Saravanadevi Sivanesan
    • 2
    Email author
  1. 1.Environmental Biotechnology DivisionCSIR-National Environmental Engineering Research Institute (NEERI)NagpurIndia
  2. 2.Environmental Health DivisionCSIR-National Environmental Engineering Research Institute (NEERI)NagpurIndia

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