Advertisement

Biological Trace Element Research

, Volume 175, Issue 2, pp 375–387 | Cite as

Protective Action of Se-Supplement Against Acute Alcoholism Is Regulated by Selenoprotein P (SelP) in the Liver

  • Zhenbiao Zhang
  • Yingfang Guo
  • Changwei Qiu
  • Ganzhen Deng
  • Mengyao GuoEmail author
Article

Abstract

Acute alcoholism is a major cause of cirrhosis and liver failure around the world. Selenium (Se) is an essential micronutrient promoting liver health in humans and animals. Selenoprotein P (SelP) is a glycoprotein secreted within the liver, which interacts with cytokines and the growth factor pathway to provide protection for hepatic cells. The present study was conducted to confirm the effect and mechanism of Se and SelP action in livers affected by acute alcoholism. In this study, a mouse model of acute alcoholism, as well as a hepatocyte model, was successfully established. The Se content of the liver was detected by atomic fluorescence spectrophotometry. The expression of messenger RNA (mRNA) was analyzed by quantitative polymerase chain reaction (qPCR). The protein expression of inflammatory factors was detected by ELISA. The other proteins were analyzed by western blotting. The results showed that pathological damage to the liver was gradually weakened by Se-supplementation, which was evaluated by hematoxylin and eosin (H&E) and TUNEL staining. Se-supplementation inhibited expression of pro-inflammatory factors TNF-α and IL-1β and promoted production of anti-inflammatory cytokine IL-10 in the liver with acute alcoholism. Se-supplementation also prevented the apoptosis of hepatocytes by suppressing the cleavage of caspases-9, 3, 6, 7, and poly(ADP-ribose) polymerase (PARP). Through correlational analysis, it was determined that the effects of Se-supplement were closely related to SelP expression, inflammatory cytokines, and apoptosis molecule production. The sienna of SelP further confirmed the protective action of Se-supplementation on the liver and that the mechanism of SelP involves the regulation of inflammatory cytokines and apoptosis molecules in acute alcoholism. These findings provide information regarding a new potential target for the treatment of acute alcoholism.

Keywords

Selenium (Se) Selenoprotein P (SelP) Acute alcoholism Liver Protective 

Notes

Acknowledgments

This work was supported by a grant from the Fundamental Research Funds for the Central Universities (No. 2662014BQ024), the National Natural Science Foundation of China (Nos. 31502130, 31472254, and 31272631), and Undergraduate Special Science and Technology Innovation of Huazhong Agricultural University (No. 2015BC009).

References

  1. 1.
    Li S, Wang SU, Guo ZG, Huang N, Zhao FR, Zhu ML, et al. (2015) Protective effect of Xingnaojia formulation on rats with brain and liver damage caused by chronic alcoholism. Exp Ther Med 10:1643–1652PubMedPubMedCentralGoogle Scholar
  2. 2.
    De Rick A, Vanheule S, Verhaeghe P (2009) Alcohol addiction and the attachment system: an empirical study of attachment style, alexithymia, and psychiatric disorders in alcoholic inpatients. Subst Use Misuse 44:99–114CrossRefPubMedGoogle Scholar
  3. 3.
    Thursz M, Morgan TR (2016) Treatment of severe alcoholic hepatitis. Gastroenterology 150:1823–34Google Scholar
  4. 4.
    Bruha R, Dvorak K, Petrtyl J (2012) Alcoholic liver disease. World J Hepatol 4:81–90CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Radosavljevic T, Mladenovic D, Jakovljevic V, Vucevic D, Rasic-Markovic A, Hrncic D, et al. (2009) Oxidative stress in liver and red blood cells in acute lindane toxicity in rats. Hum Exp Toxicol 28:747–757CrossRefPubMedGoogle Scholar
  6. 6.
    Yu C, Mei XT, Zheng YP, Xu DH (2014) Zn(II)-curcumin protects against hemorheological alterations, oxidative stress and liver injury in a rat model of acute alcoholism. Environ Toxicol Pharmacol 37:729–737CrossRefPubMedGoogle Scholar
  7. 7.
    Ju C, Mandrekar P (2015) Macrophages and alcohol-related liver inflammation. Alcohol Res Curr Rev 37:251–262Google Scholar
  8. 8.
    Zhang Z, Gao X, Cao Y, Jiang H, Wang T, Song X, et al. (2015) Selenium deficiency facilitates inflammation through the regulation of TLR4 and TLR4-related signaling pathways in the mice uterus. Inflammation 38:1347–1356CrossRefPubMedGoogle Scholar
  9. 9.
    Li W, Guo M, Liu Y, Mu W, Deng G, Li C, et al. (2015) Selenium induces an anti-tumor effect via inhibiting intratumoral angiogenesis in a mouse model of transplanted canine mammary tumor cells. Biol Trace Elem Res 171(2):371–379CrossRefPubMedGoogle Scholar
  10. 10.
    Liu C, Jing F, Liu C, Li S (2015) The role of nitric oxide and autophagy in liver injuries induced by selenium deficiency in chickens. RSC Adv 5:50549–50556CrossRefGoogle Scholar
  11. 11.
    Jiang Z-H, Khoso PA, Yao H-D, Zhang Z-W, Zhang X-Y, Xu S-w (2015) SelW regulates inflammation-related cytokines in response to H2O2 in Se-deficient chicken liver. RSC Adv 5:37896–37905CrossRefGoogle Scholar
  12. 12.
    Fischer A, Pallauf J, Gohil K, Weber SU, Packer L, Rimbach G (2001) Effect of selenium and vitamin E deficiency on differential gene expression in rat liver. Biochem Biophys Res Commun 285:470–475CrossRefPubMedGoogle Scholar
  13. 13.
    Yao HD, Wu Q, Zhang ZW, Li S, Wang XL, Lei XG, Xu SW (2013) Selenoprotein W serves as an antioxidant in chicken myoblasts. Biochim Biophys Acta 1830(4):3112–3120CrossRefPubMedGoogle Scholar
  14. 14.
    Yao HD, Wu Q, Zhang ZW, Zhang JL, Li S, Huang JQ, Ren FZ, Xu SW, Wang XL, Lei XG (2013) Gene expression of endoplasmic reticulum resident selenoproteins correlates with apoptosis in various muscles of se-deficient chicks. J Nutr 143(5):613–619CrossRefPubMedPubMedCentralGoogle Scholar
  15. 15.
    Stranges S, Galletti F, Farinaro E, D’Elia L, Russo O, Iacone R, et al. (2011) Associations of selenium status with cardiometabolic risk factors: an 8-year follow-up analysis of the Olivetti Heart study. Atherosclerosis 217:274–278CrossRefPubMedGoogle Scholar
  16. 16.
    Marsh JA, Combs GF Jr, Whitacre ME, Dietert RR (1986) Effect of selenium and vitamin E dietary deficiencies on chick lymphoid organ development. Proc Soc Exp Biol Med Soc Exp Biol Med 182:425–436CrossRefGoogle Scholar
  17. 17.
    Nourbakhsh M, Ahmadpour F, Chahardoli B, Malekpour-Dehkordi Z, Nourbakhsh M, Hosseini-Fard SR, et al. (2016) Selenium and its relationship with selenoprotein P and glutathione peroxidase in children and adolescents with Hashimoto’s thyroiditis and hypothyroidism. J Trace Elem Med Biol: Organ Soc Miner Trace Elem 34:10–14CrossRefGoogle Scholar
  18. 18.
    Burk RF, Hill KE (2015) Regulation of selenium metabolism and transport. Annu Rev Nutr 35:109–134CrossRefPubMedGoogle Scholar
  19. 19.
    Gromer S, Eubel JK, Lee BL, Jacob J (2005) Human selenoproteins at a glance. Cell Mol Life Sci 62:2414–2437CrossRefPubMedGoogle Scholar
  20. 20.
    Mostert V (2000) Selenoprotein P: properties, functions, and regulation. Arch Biochem Biophys 376:433–438CrossRefPubMedGoogle Scholar
  21. 21.
    Burk RF, Hill KE, Motley AK (2003) Selenoprotein metabolism and function: evidence for more than one function for selenoprotein P. J Nutr 133:1517s-20sPubMedGoogle Scholar
  22. 22.
    Misu H, Takamura T, Takayama H, Hayashi H, Matsuzawa-Nagata N, Kurita S, et al. (2010) A liver-derived secretory protein, selenoprotein P, causes insulin resistance. Cell Metab 12:483–495CrossRefPubMedGoogle Scholar
  23. 23.
    Yang SJ, Hwang SY, Choi HY, Yoo HJ, Seo JA, Kim SG, et al. (2011) Serum selenoprotein P levels in patients with type 2 diabetes and prediabetes: implications for insulin resistance, inflammation, and atherosclerosis. J Clin Endocrinol Metab 96:E1325–E1329CrossRefPubMedGoogle Scholar
  24. 24.
    Barve A, Khan R, Marsano L, Ravindra KV, McClain C (2008) Treatment of alcoholic liver disease. Ann Hepatol 7:5–15PubMedGoogle Scholar
  25. 25.
    Williams R (2008) The pervading influence of alcoholic liver disease in hepatology. Alcohol Alcohol 43:393–397CrossRefPubMedGoogle Scholar
  26. 26.
    Kohrle J, Gartner R (2009) Selenium and thyroid. Best Pract Res Clin Endocrinol Metab 23:815–827CrossRefPubMedGoogle Scholar
  27. 27.
    Effraimidis G, Wiersinga WM (2014) Mechanisms in endocrinology: autoimmune thyroid disease: old and new players. Eur J Endocrinol/Eur Fed Endocrine Soc 170:R241–R252CrossRefGoogle Scholar
  28. 28.
    Hasunuma R, Ogawa T, Kawanishi Y (1982) Fluorometric determination of selenium in nanogram amounts in biological materials using 2,3-diaminonaphthalene. Anal Biochem 126:242–245CrossRefPubMedGoogle Scholar
  29. 29.
    Kohrle J, Jakob F, Contempre B, Dumont JE (2005) Selenium, the thyroid, and the endocrine system. Endocr Rev 26:944–984CrossRefPubMedGoogle Scholar
  30. 30.
    Chan DS, Wong M (1997) Multidisciplinary handbook on pediatric nutritional support. Am J Health Syst Pharm: AJHP: Off J Am Soc Health Syst Pharm 54:1355Google Scholar
  31. 31.
    Liu Y, Qiu C, Li W, Mu W, Li C, Guo M (2016) Selenium plays a protective role in staphylococcus aureus-induced endometritis in the uterine tissue of rats. Biol Trace Elem Res:1–9Google Scholar
  32. 32.
    Shilo S, Pardo M, Aharoni-Simon M, Glibter S, Tirosh O (2008) Selenium supplementation increases liver MnSOD expression: molecular mechanism for hepato-protection. J Inorg Biochem 102:110–118CrossRefPubMedGoogle Scholar
  33. 33.
    Rua RM, Ojeda ML, Nogales F, Rubio JM, Romero-Gomez M, Funuyet J, et al. (2014) Serum selenium levels and oxidative balance as differential markers in hepatic damage caused by alcohol. Life Sci 94:158–163CrossRefPubMedGoogle Scholar
  34. 34.
    Adachi M, Brenner DA (2005) Clinical syndromes of alcoholic liver disease. Dig Dis 23:255–263CrossRefPubMedGoogle Scholar
  35. 35.
    Bataller R, Brenner DA (2005) Liver fibrosis. J Clin Invest 115:209–218CrossRefPubMedPubMedCentralGoogle Scholar
  36. 36.
    Guo M, Lv T, Liu F, Yan H, Wei T, Cai H, et al. (2013) Dietary selenium influences calcium release and activation of MLCK in uterine smooth muscle of rats. Biol Trace Elem Res 154:127–133CrossRefPubMedPubMedCentralGoogle Scholar
  37. 37.
    Ramaiah S, Rivera C, Arteel G (2004) Early-phase alcoholic liver disease: an update on animal models, pathology, and pathogenesis. Int J Toxicol 23:217–231CrossRefPubMedGoogle Scholar
  38. 38.
    Zhang W, Zhang R, Wang T, Jiang H, Guo M, Zhou E, et al. (2014) Selenium inhibits LPS-induced pro-inflammatory gene expression by modulating MAPK and NF-kappaB signaling pathways in mouse mammary epithelial cells in primary culture. Inflammation 37:478–485CrossRefPubMedGoogle Scholar
  39. 39.
    Wei Z, Yao M, Li Y, He X, Yang Z (2014) Dietary selenium deficiency exacerbates lipopolysaccharide-induced inflammatory response in mouse mastitis models. Inflammation 37:1925–1931CrossRefPubMedGoogle Scholar
  40. 40.
    Diamond ML, Boles JA, Ritter AC, Failla MD, Conley YP, Kochanek PM, et al. (2014) In response to comments on IL-1beta associations with posttraumatic epilepsy development: a genetics and biomarker cohort study. Epilepsia 55:1313–1314CrossRefPubMedGoogle Scholar
  41. 41.
    Lima AA, Spinola LG, Baccan G, Correia K, Oliva M, Vasconcelos JF, et al. (2014) Evaluation of corticosterone and IL-1beta, IL-6, IL-10 and TNF-alpha expression after 670-nm laser photobiomodulation in rats. Lasers Med Sci 29:709–715CrossRefPubMedGoogle Scholar
  42. 42.
    Hagenlocher Y, Hosel A, Bischoff SC, Lorentz A (2016) Cinnamon extract reduces symptoms, inflammatory mediators and mast cell markers in murine IL-10(-/-) colitis. J Nutr Biochem 30:85–92CrossRefPubMedGoogle Scholar
  43. 43.
    Into T, Shibata K (2005) Apoptosis signal-regulating kinase 1-mediated sustained p38 mitogen-activated protein kinase activation regulates mycoplasmal lipoprotein- and staphylococcal peptidoglycan-triggered toll-like receptor 2 signalling pathways. Cell Microbiol 7:1305–1317CrossRefPubMedGoogle Scholar
  44. 44.
    Pandey AK, Sodhi A (2011) Recombinant YopJ induces apoptotic cell death in macrophages through TLR2. Mol Immunol 48:392–398CrossRefPubMedGoogle Scholar
  45. 45.
    Katunuma N, Ishidoh K, Sakurai J, Oda M, Kamemura N (2008) Reciprocal relationship between the apoptosis pathway mediated by executioner caspases and the physiological NAD synthesis pathway. Adv Enzym Regul 48:19–30CrossRefGoogle Scholar
  46. 46.
    Schomburg L, Schweizer U, Holtmann B, Flohe L, Sendtner M, Kohrle J (2003) Gene disruption discloses role of selenoprotein P in selenium delivery to target tissues. Biochem J 370:397–402CrossRefPubMedPubMedCentralGoogle Scholar
  47. 47.
    Hill KE, Zhou J, McMahan WJ, Motley AK, Atkins JF, Gesteland RF, et al. (2003) Deletion of selenoprotein P alters distribution of selenium in the mouse. J Biol Chem 278:13640–13646CrossRefPubMedGoogle Scholar
  48. 48.
    Burk RF, Hill KE, Read R, Bellew T (1991) Response of rat selenoprotein P to selenium administration and fate of its selenium. Am J Phys 261:E26–E30Google Scholar
  49. 49.
    Barrett CW, Reddy VK, Short SP, Motley AK, Lintel MK, Bradley AM, et al. (2015) Selenoprotein P influences colitis-induced tumorigenesis by mediating stemness and oxidative damage. J Clin Invest 125:2646–2660CrossRefPubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Zhenbiao Zhang
    • 1
  • Yingfang Guo
    • 1
  • Changwei Qiu
    • 1
  • Ganzhen Deng
    • 1
  • Mengyao Guo
    • 1
    • 2
    Email author
  1. 1.Department of Clinical Veterinary Medicine, College of Veterinary MedicineHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.College of Veterinary MedicineHuazhong Agricultural UniversityWuhanPeople’s Republic of China

Personalised recommendations