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Biological Trace Element Research

, Volume 173, Issue 2, pp 483–491 | Cite as

Dietary High Fluorine Alters Intestinal Microbiota in Broiler Chickens

  • Qin Luo
  • Hengmin CuiEmail author
  • Xi Peng
  • Jing Fang
  • Zhicai Zuo
  • Junliang Deng
  • Juan Liu
  • Yubing Deng
Article

Abstract

This study investigated the effects of dietary high fluorine on ileal and cecal microbiota in broiler chickens. Two hundred eighty 1-day-old broiler chickens were randomly assigned to four groups and raised for 42 days. The control group was fed a corn-soybean basal diet (fluorine 22.6 mg/kg). The other three groups were fed the same basal diet, but supplemented with 400, 800, and 1200 mg/kg fluorine (high fluorine groups I, II, and III), administered in the form of sodium fluoride. The microbiota of ileal and cecal digesta was assessed with plate counts and polymerase chain reaction-based denaturing gradient gel electrophoresis (PCR-DGGE). It was found that, compared with those in the control group, the counts of Lactobacillus spp. and Bifidobacterium spp. were markedly decreased (P < 0.01 or P < 0.05), whereas the counts of Escherichia coli and Enterococcus spp. were significantly increased (P < 0.01 or P < 0.05) in the high fluorine groups II and III. PCR-DGGE analysis showed that the number of DGGE bands, similarity, and Shannon index of ileal and cecal bacteria were markedly reduced in the high fluorine groups II and III from 21 to 42 days. Sequencing analysis revealed that the composition of the intestinal microbiota was altered in the high fluorine groups. In conclusion, dietary fluorine in the range of 800–1200 mg/kg obviously altered the bacterial counts, and the diversity and composition of intestinal microbiota in broiler chickens, a finding which implies that dietary high fluorine can disrupt the natural balance and structure of the intestinal microbiota.

Keywords

Broiler chickens Fluorine Intestinal microbiota PCR-DGGE Sequencing 

Notes

Acknowledgments

The study was supported by the program for Changjiang Scholars and Innovative University Research Teams (IRT 0848), and the Shuangzhi Project of Sichuan Agricultural University (03570327; 03571198).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Qin Luo
    • 1
  • Hengmin Cui
    • 1
    Email author
  • Xi Peng
    • 1
  • Jing Fang
    • 1
  • Zhicai Zuo
    • 1
  • Junliang Deng
    • 1
  • Juan Liu
    • 1
  • Yubing Deng
    • 1
  1. 1.Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary MedicineSichuan Agricultural UniversityYa’anChina

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