Effects of Fluoride on the Expression of p38MAPK Signaling Pathway-Related Genes and Proteins in Spleen Lymphocytes of Mice
- 332 Downloads
This study investigated the effects of sodium fluoride on the expression of p38MAPK signaling pathway-related genes and proteins in the spleen lymphocytes of mice, revealing the mechanism of the toxicity of fluoride to the immune system. The spleen lymphocytes, isolated from mice consuming different NaF doses (0, 50, 100, and 150 mg/L) for 60 days, were cultured in medium with bacteria lipopolysaccharide, and the cells’ proliferation ability was analyzed through MTT; real-time PCR detected the change of MLK3/MKK6/p38MAPK/MSK1/ATF1 on mRNA, and the difference of protein expression of MKK6/p38MAPK were detected through the Western blotting. The results suggested that the proliferation ability of spleen lymphocytes isolated from mice consuming different NaF doses is lower, and the expression of genes and proteins of MKK6/p38MAPK showed a decreasing trend. These results demonstrate that fluoride can suppress the activation of p38MAPK pathway in mice spleen lymphocytes and further influences the function of the immune system.
KeywordsFluoride Spleen lymphocytes p38MAPK Gene expression Protein expression
This paper was supported by the Natural Science Foundation of Shanxi Province of China (2014011027) and the National Natural Science Foundation of China (31001089).
Compliance with Ethical Standards
All animal procedures followed the National Institutes of Health Animal Care and Use Guidelines, Ministry of Environment, Forest and Climate Change.
Conflict of Interest
The authors declare that there is no conflict of interests.
- 2.Podder S, Chattopadhyay A, Bhattacharya S, et al. (2010) Histopathology and cell cycle alteration in the spleen of mice from low and high dose of sodium fluoride. Fluoride 43(4):237–245Google Scholar
- 3.Wang J, Hong J, Li J, et al. (2002) Effect of high fluoride and low protein on tooth matrix development in goats. Fluoride 35(1):51–55Google Scholar
- 4.Chen T, Cui Y, Bai CM, et al. (2009) Increased apoptotic lymphocyte population in the spleen of young chickens fed diets high in fluorine. Fluoride 42(2):94–100Google Scholar
- 9.Jin-ye MU, Chen XG (2002) The progress of the study on the mitogen-activated protein kinase pathways. Chin Bull Life Sci 14(4):208–211Google Scholar
- 11.Xu G, Wang JM, Ma SH, et al. (2014) Effects of fluoride on the expression of ERK signaling pathway associated gene and proteins in splenic lymphocytes of mouse. Acta Vet Zootech Sin 45(11):1888–1894Google Scholar
- 13.Zhang AJ, Yang DM, Bai SQ (2002) The immune toxicity of fluoride. Chin J Ctrl Endem Dis 1(1):30–32Google Scholar