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Effects of Fluoride on the Expression of p38MAPK Signaling Pathway-Related Genes and Proteins in Spleen Lymphocytes of Mice

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Abstract

This study investigated the effects of sodium fluoride on the expression of p38MAPK signaling pathway-related genes and proteins in the spleen lymphocytes of mice, revealing the mechanism of the toxicity of fluoride to the immune system. The spleen lymphocytes, isolated from mice consuming different NaF doses (0, 50, 100, and 150 mg/L) for 60 days, were cultured in medium with bacteria lipopolysaccharide, and the cells’ proliferation ability was analyzed through MTT; real-time PCR detected the change of MLK3/MKK6/p38MAPK/MSK1/ATF1 on mRNA, and the difference of protein expression of MKK6/p38MAPK were detected through the Western blotting. The results suggested that the proliferation ability of spleen lymphocytes isolated from mice consuming different NaF doses is lower, and the expression of genes and proteins of MKK6/p38MAPK showed a decreasing trend. These results demonstrate that fluoride can suppress the activation of p38MAPK pathway in mice spleen lymphocytes and further influences the function of the immune system.

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Acknowledgments

This paper was supported by the Natural Science Foundation of Shanxi Province of China (2014011027) and the National Natural Science Foundation of China (31001089).

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Authors and Affiliations

  1. College of Animal Science and Veterinary Medicine, Shanxi Agriculture University, Taigu, Jinzhong, Shanxi, (030800), China

    Zeyu Shi, Yaqi Zhan, Junxing Zhao, Jinming Wang & Haili Ma

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  1. Zeyu Shi
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  2. Yaqi Zhan
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  3. Junxing Zhao
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  4. Jinming Wang
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  5. Haili Ma
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Correspondence to Haili Ma.

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All animal procedures followed the National Institutes of Health Animal Care and Use Guidelines, Ministry of Environment, Forest and Climate Change.

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The authors declare that there is no conflict of interests.

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Shi, Z., Zhan, Y., Zhao, J. et al. Effects of Fluoride on the Expression of p38MAPK Signaling Pathway-Related Genes and Proteins in Spleen Lymphocytes of Mice. Biol Trace Elem Res 173, 333–338 (2016). https://doi.org/10.1007/s12011-016-0656-9

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  • DOI: https://doi.org/10.1007/s12011-016-0656-9

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