Biological Trace Element Research

, Volume 173, Issue 2, pp 333–338 | Cite as

Effects of Fluoride on the Expression of p38MAPK Signaling Pathway-Related Genes and Proteins in Spleen Lymphocytes of Mice

  • Zeyu Shi
  • Yaqi Zhan
  • Junxing Zhao
  • Jinming Wang
  • Haili MaEmail author


This study investigated the effects of sodium fluoride on the expression of p38MAPK signaling pathway-related genes and proteins in the spleen lymphocytes of mice, revealing the mechanism of the toxicity of fluoride to the immune system. The spleen lymphocytes, isolated from mice consuming different NaF doses (0, 50, 100, and 150 mg/L) for 60 days, were cultured in medium with bacteria lipopolysaccharide, and the cells’ proliferation ability was analyzed through MTT; real-time PCR detected the change of MLK3/MKK6/p38MAPK/MSK1/ATF1 on mRNA, and the difference of protein expression of MKK6/p38MAPK were detected through the Western blotting. The results suggested that the proliferation ability of spleen lymphocytes isolated from mice consuming different NaF doses is lower, and the expression of genes and proteins of MKK6/p38MAPK showed a decreasing trend. These results demonstrate that fluoride can suppress the activation of p38MAPK pathway in mice spleen lymphocytes and further influences the function of the immune system.


Fluoride Spleen lymphocytes p38MAPK Gene expression Protein expression 



This paper was supported by the Natural Science Foundation of Shanxi Province of China (2014011027) and the National Natural Science Foundation of China (31001089).

Compliance with Ethical Standards

All animal procedures followed the National Institutes of Health Animal Care and Use Guidelines, Ministry of Environment, Forest and Climate Change.

Conflict of Interest

The authors declare that there is no conflict of interests.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.College of Animal Science and Veterinary MedicineShanxi Agriculture UniversityJinzhongChina

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