Effect of Selenium on the Levels of Cytokines and Trace Elements in Toxin-Mediated Oxidative Stress in Male Rats
- 437 Downloads
Selenium is an essential cofactor in the key enzymes involved in cellular antioxidant defense. This study was designed to investigate the protective effects of selenium on mercury chloride (HgCl2)-induced toxicity. Male Wistar rats were randomly divided into four groups of six animals each. The first group was control; the second group was treated with mercuric chloride (HgCl2: 50 mg/kg/bw). The third group was treated with sodium selenite (Se 0.2 mg/kg/bw), and the fourth group received Se (0.2 mg/kg/bw) plus HgCl2 (50 mg/kg for 24 h). The influence of Se on mercury induced levels of malondialdehyde (MDA) and the activity of superoxide dismutase (SOD) and zinc, copper, and iron in serum of rats were observed. The serum MDA, SOD, zinc, and iron concentrations were found to be statistically different among the control and toxin-treated group. The serum levels of IL-6, IL-10, and TNF-α were also measured. There was a significant decrease in the levels of TNF-α, IL-6, and IL-10 in toxin-treated group II compared with that of the control group (p < 0.05). A significant increase in the serum levels of inflammatory cytokines IL-6, TNF-α, and IL-10 after administration of Se seemed to counteract some of the damage, as indicated by differences in the serum concentrations of major elements.
KeywordsSelinium Serum Toxicity Trace elements Inflammation Cytokines
This research project was supported by the “Research Center of the Center for Female Scientific and Medical Colleges,” Deanship of Scientific Research, King
, Saud University, Riyadh, Saudi Arabia.
- 3.Agarwal R, Raisuddin S, Tewari S, Goel SK, Raizada RB, Behari JR (2010a) Evaluation of comparative effect of pre- and posttreatment of selenium on mercury-induced oxidative stress, histological alterations, and metallothionein mRNA expression in rats. J Biochem Mol Toxicol 24:123–135PubMedGoogle Scholar
- 8.Taber KH, Hurley RA (2008) Mercury exposure: effects across the lifespan. J Neuropsychiatry Clin Neurosci 20:iv–389Google Scholar
- 9.Liu WW, Jiang CQ, Hu ZB, Zhang C, Xu QR, Zhou G (2006) Mercury concentration in cerebrospinal fluid in patients with chronic mercury poisoning. Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za ZhiZhonghua Laodong Weisheng Zhiyebing Zazhi Chin J Ind Hyg Occup Dis 24:403–405Google Scholar
- 17.Nava M, Romero F, Quiroz Y, Parra G, Bonet L, Rodriguez-Iturbe B (2000) Melatonin attenuates acute renal failure and oxidative stress induced by mercuric chloride in rats. Am J Physiol Ren Physiol 279:F910–F918Google Scholar
- 21.Kouba A, Velisek J, Stara A, Masojidek J, Kozak P (2014) Supplementation with sodium selenite and selenium-enriched microalgae biomass show varying effects on blood enzymes activities, antioxidant response, and accumulation in common barbel (Barbus barbus). Biomed Res Int 2014:408270PubMedPubMedCentralCrossRefGoogle Scholar
- 38.Uzunhisarcikli M, Aslanturk A, Kalender S, Apaydin FG, Bas H (2015) Mercuric chloride induced hepatotoxic and hematologic changes in rats: the protective effects of sodium selenite and vitamin E. Toxicol Ind HealthGoogle Scholar