Study on Biological Effects of La3+ on Rat Liver Mitochondria by Microcalorimetric and Spectroscopic Methods
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The effects of lanthanum on heat production of mitochondria isolated from Wistar rat liver were investigated with microcalorimetry; simultaneously, the effects on mitochondrial swelling and membrane potential (Δψ) were determined by spectroscopic methods. La3+ showed only inhibitory action on mitochondrial energy turnover with IC50 being 55.8 μmol L−1. In the spectroscopic experiments, La3+, like Ca2+, induced rat liver mitochondrial swelling and decreased membrane potential (Δψ), which was inhibited by the specific permeability transition inhibitor, cyclosporine A (CsA). The induction ability of La3+ was stronger than that of Ca2+. These results demonstrated that La3+ had some biotoxicity effect on mitochondria; the effects of La3+ and Ca2+ on rat liver mitochondrial membrane permeability transition (MPT) are different, and La represents toxic action rather than Ca analogy.
KeywordsMitochondria Metabolism La3+ Permeability transition
We gratefully acknowledge the financial support of the project by the National Natural Science Foundation of China (No. 21173026, 21225313) and the Key Program of Natural Science Foundation of Hubei Province (No. 2013CFA107).
Conflict of Interest
The authors declare no conflict of interest.
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