Biological Trace Element Research

, Volume 159, Issue 1–3, pp 425–433 | Cite as

Zinc Chloride Inhibits Human Lens Epithelial Cell Migration and Proliferation Involved in TGF-β1 and TNF-α Signaling Pathways in HLE B-3 Cells

  • Yuxiang Du
  • Dadong Guo
  • Qiuxin Wu
  • Dongmei Liu
  • Hongsheng BiEmail author


Zinc is one of the most abundant essential elements in the human body, which is an essential, coenzyme-like component of many enzymes, and is indispensable to their functions. However, high levels of zinc ions can lead to cell damage. In the present study, we explored the effects of high concentrations of zinc chloride (ZnCl2) on lens epithelial cell proliferation and migration and further investigated the effects of different concentrations of ZnCl2 on caspase-9 and caspase-12, transforming growth factor-beta 1 (TGF-β1), and tumor necrosis factor-alpha (TNF-α). We found that ZnCl2 could inhibit human lens epithelial (HLE) B-3 cell migration and induce apoptosis/necrosis. In addition, ZnCl2 can efficiently decrease the expressions of caspase-9 and caspase-12, increase the expression of TNF-α at both gene and protein levels, and thus induces cell death. Taken together, our results indicate that ZnCl2 can inhibit HLE B-3 cell migration and proliferation by decreasing the expression of TGF-β1 and increasing the expression of TNF-α and finally lead to HLE B-3 cell death.


Zinc ion Lens epithelial cell Caspase Transforming growth factor Tumor necrosis factor 



This work was supported by the Natural Science Foundation of Shandong province (ZR2010HM032).

Conflict of Interest

The authors declare that they have no competing interests.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Yuxiang Du
    • 1
  • Dadong Guo
    • 2
  • Qiuxin Wu
    • 3
  • Dongmei Liu
    • 2
  • Hongsheng Bi
    • 2
    • 3
    Email author
  1. 1.Shandong University of Traditional Chinese MedicineJinanChina
  2. 2.Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases of Shandong province; Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Therapy of Ocular Diseases in Universities of Shandong; Eye Institute of Shandong University of Traditional Chinese MedicineJinanChina
  3. 3.Affiliated Eye Hospital of Shandong University of Traditional Chinese MedicineJinanChina

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