Biological Trace Element Research

, Volume 158, Issue 2, pp 224–229

Effects of Selenium on Liver and Muscle Contents and Urinary Excretion of Zinc, Copper, Iron and Manganese

  • María José Monedero-Prieto
  • José María González-Pérez
  • Emilio González-Reimers
  • Onán Hernández-Pérez
  • María Monereo-Muñoz
  • Luis Galindo-Martín
  • Geraldine Quintero-Platt
  • Pedro Abreu-González


Selenium is a main component of glutathione peroxidase (GPX), a key antioxidant enzyme. Other elements, such as zinc, copper, manganese and iron, are also involved in the pathogenesis of oxidative damage as well as in other important metabolic pathways. The effects of selenium supplementation on the metabolism of these elements have yield controversial results .The aim of this study is to analyse the effects of selenium supplementation on liver, muscle and urinary excretion of zinc, copper, iron and manganese in a situation of oxidative stress, such as protein deficiency. The experimental design included four groups of adult male Sprague–Dawley rats, which received the Lieber–DeCarli control diet, an isocaloric 2 % protein-containing diet and another similar two groups to which selenomethionine (6 mg/l liquid diet) was added. After sacrifice (5 weeks later), muscle, liver and serum selenium were determined, as well as muscle, liver and urinary zinc, copper, manganese and iron and liver GPX activity and liver malondialdehyde. Selenium addition led to decreased liver copper, increased muscle copper, increased copper excretion and increased liver iron, whereas zinc and manganese parameters were essentially unaltered. Muscle, liver and serum selenium were all significantly correlated with liver GPX activity.


Selenium Copper Zinc Iron Manganese Liver Muscle Lipid peroxidation GPX 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • María José Monedero-Prieto
    • 1
  • José María González-Pérez
    • 1
  • Emilio González-Reimers
    • 1
  • Onán Hernández-Pérez
    • 1
  • María Monereo-Muñoz
    • 1
  • Luis Galindo-Martín
    • 2
  • Geraldine Quintero-Platt
    • 1
  • Pedro Abreu-González
    • 3
  1. 1.Servicio de Medicina InternaHospital Universitario de CanariasTenerifeSpain
  2. 2.Dpto. de Química AnalíticaUniversidad de La LagunaTenerifeSpain
  3. 3.Dpto. de Fisiología, Facultad de MedicinaUniversidad de la LagunaTenerifeSpain

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