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Biological Trace Element Research

, Volume 161, Issue 1, pp 101–106 | Cite as

Effects of Resistance Exercise on Iron Absorption and Balance in Iron-Deficient Rats

  • Takako Fujii
  • Tatsuhiro Matsuo
  • Koji Okamura
Article

Abstract

We have previously reported that resistance exercise improved the iron status in iron-deficient rats. The current study investigated the mechanisms underlying this exercise-related effect. Male 4-week-old rats were divided into a group sacrificed at the start (week 0) (n = 7), a group maintained sedentary for 6 weeks (S) or a group that performed exercise for 6 weeks (E), and all rats in the latter groups were fed an iron-deficient diet (12 mg iron/kg) for 6 weeks. The rats in the E group performed climbing exercise (5 min × 6 sets/day, 3 days/week). Compared to the week 0 rats, the rats in the S and E groups showed lower tissue iron content, and the hematocrit, hemoglobin, plasma iron, and transferrin saturation values were all low. However, the tissue iron content and blood iron status parameters, and the whole body iron content measured using the whole body homogenates of the rats, did not differ between the S group and the E group. The messenger RNA (mRNA) expression levels of hepcidin, duodenal cytochrome b, divalent metal transporter 1, and ferroportin 1 did not differ between the S group and the E group. The apparent absorption of iron was significantly lower in the E group than in the S group. Therefore, it was concluded that resistance exercise decreases iron absorption, whereas the whole body iron content is not affected, and an increase in iron recycling in the body seems to be responsible for this effect.

Keywords

Resistance exercise Iron deficiency Iron absorption Iron balance Iron recycle 

References

  1. 1.
    Ehn L, Carlmark B, Hoglund S (1980) Iron status in athletes involved in intense physical activity. Med Sci Sports Exerc 12:61–64PubMedCrossRefGoogle Scholar
  2. 2.
    Matsuo T, Suzuki M (2000) Dumbbell exercise improves non-anemic iron deficiency in young women without iron supplementation. Health Sci 16:236–243Google Scholar
  3. 3.
    Matsuo T (2004) Effects of resistance exercise on iron metabolism in iron-adequate or iron-deficient rats. Korean J Exerc Nutr 8:1–15Google Scholar
  4. 4.
    Matsuo T, Suzuki H, Suzuki M (2000) Resistance exercise increases the capacity of heme biosynthesis more than aerobic exercise in rats. J Clin Biochem Nutr 29:19–27CrossRefGoogle Scholar
  5. 5.
    Fujii T, Matsuo T, Okamura K (2012) The effects of resistance exercise and post-exercise meal timing on the iron status in iron deficient rats. Biol Trace Elem Res 147:200–205PubMedCrossRefGoogle Scholar
  6. 6.
    Matsuo T, Kang HS, Suzuki H (2002) Voluntary resistance exercise improves blood hemoglobin concentration in severely iron-deficient rats. J Nutr Sci Vitaminol 48:161–164PubMedCrossRefGoogle Scholar
  7. 7.
    Fujii T, Asai T, Matsuo T, Okamura K (2011) Effect of resistance exercise on iron status in moderately iron deficient rats. Biol Trace Elem Res 144:983–991PubMedCrossRefGoogle Scholar
  8. 8.
    Nachtigall D, Nielsen P, Fischer R, Engelhardt R, Gabbe EE (1996) Iron deficiency in distance runners. A reinvestigation using Fe-labelling and non-invasive liver iron quantification. Int J Sports Med 17:473–479PubMedCrossRefGoogle Scholar
  9. 9.
    Ruckman KS, Sherman AR (1981) Effects of exercise on iron and copper metabolism in rats. J Nutr 111:1593–1601PubMedGoogle Scholar
  10. 10.
    Ganz T (2011) Hepcidin and iron regulation, 10 years later. Blood 117:4425–4433PubMedCrossRefPubMedCentralGoogle Scholar
  11. 11.
    Nicolas G, Chauvet C, Viatte L, Danan JL, Bigard X, Devaux I, Beaumont C, Kahn A, Vaulont S (2002) The gene encoding the iron regulatory peptide hepcidin is regulated by anemia, hypoxia, and inflammation. J Clin Invest 110:1037–1044PubMedCrossRefPubMedCentralGoogle Scholar
  12. 12.
    Peeling P, Dawson B, Goodman C et al (2009) Cumulative effects of consecutive running sessions on hemolysis, inflammation and hepcidin activity. Eur J Appl Physiol 106:51–59PubMedCrossRefGoogle Scholar
  13. 13.
    Roecker L, Meier-Buttermilch R, Brechtel L, Nemeth E, Ganz T (2005) Iron-regulatory protein hepcidin is increased in female athletes after a marathon. Eur J Appl Physiol 95:569–571PubMedCrossRefGoogle Scholar
  14. 14.
    Peeling P, Dawson B, Goodman C, Landers G, Wiegerinck ET, Swinkels DW, Trinder D (2009) Effects of exercise on hepcidin response and iron metabolism during recovery. Int J Sport Nutr Exerc Metab 19:583–597PubMedGoogle Scholar
  15. 15.
    Peeling P, Dawson B, Goodman C, Landers G, Wiegerinck ET, Swinkels DW, Trinder D (2009) Cumulative effects of consecutive running sessions on hemolysis, inflammation and hepcidin activity. Eur J Appl Physiol 106:51–59PubMedCrossRefGoogle Scholar
  16. 16.
    Robson-Ansley P, Walshe I, Ward D (2011) The effect of carbohydrate ingestion on plasma interleukin-6, hepcidin and iron concentrations following prolonged exercise. Cytokine 53:196–200PubMedCrossRefGoogle Scholar
  17. 17.
    Liu YQ, Chang YZ, Zhao B, Wang HT, Duan XL (2011) Does hepatic hepcidin play an important role in exercise-associated anemia in rats? Int J Sport Nutr Exerc Metab 21:19–26PubMedGoogle Scholar
  18. 18.
    Reeves PG, Nielsen FH, Fahey GC Jr (1993) AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr 123:1939–1951PubMedGoogle Scholar
  19. 19.
    Fujii T, Matsuo T, Okamura K (2011) Effect of a high protein diet on the anemia mitigating effect of resistance exercise in rats. Segment Journals 2:NS/1561.Google Scholar
  20. 20.
    International Nutritional Anemia Consultative Group (1985) Measurements of iron status. The Nutrition Foundation, Washington, DC, pp 12–22Google Scholar
  21. 21.
    Perkkiö MV, Jansson LT, Henderson S, Refino C, Brooks GA, Dallman PR (1985) Work performance in the iron-deficient rat: improved endurance with exercise training. Am J Physiol 249:E306–E311PubMedGoogle Scholar
  22. 22.
    Ming Qian Z, Sheng Xiao D, Kui Liao Q, Ping Ho K (2002) Effect of different durations of exercise on transferrin-bound iron uptake by rat erythroblast. J Nutr Biochem 13:47–54PubMedCrossRefGoogle Scholar
  23. 23.
    Banzet S, Sanchez H, Chapot R, Bigard X, Vaulont S, Koulmann N (2012) Interleukin-6 contributes to hepcidin mRNA increase in response to exercise. Cytokine 58:158–161PubMedCrossRefGoogle Scholar
  24. 24.
    Newlin MK, Williams S, McNamara T, Tjalsma H, Swinkels DW, Haymes EM (2012) The effects of acute exercise bouts on hepcidin in women. Int J Sport Nutr Exerc Metab 22:79–88PubMedGoogle Scholar
  25. 25.
    Troadec MB, Lainé F, Daniel V et al (2009) Daily regulation of serum and urinary hepcidin is not influenced by submaximal cycling exercise in humans with normal iron metabolism. Eur J Appl Physiol 106:435–443PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Exercise Nutrition Laboratory, Graduate School of Sport SciencesOsaka University of Health and Sport SciencesOsakaJapan
  2. 2.Faculty of AgricultureKagawa UniversityKagawaJapan

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