Biological Trace Element Research

, Volume 160, Issue 2, pp 250–257 | Cite as

Hepatoprotective Effect of Arctium lappa Root Extract on Cadmium Toxicity in Adult Wistar Rats

  • Fabricia de Souza PredesEmail author
  • Maria Aparecida da Silva Diamante
  • Mary Ann Foglio
  • Camila Almeida Camargo
  • Hiroshi Aoyama
  • Silvio Cesar Miranda
  • Bread Cruz
  • Maria Cristina Cintra Gomes Marcondes
  • Heidi Dolder


This study was performed to determine the effects of Arctium lappa (Al) to protect against cadmium damage in the rat liver. Male rats received a single i.p. dose of CdCl2 (1.2 mg/kg body weight (BW)) with or without Al extract administered daily by gavage (300 mg/kg BW) for 7 or 56 days. After 7 days, Al caused plasma transaminase activity to diminish in groups Al (glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT)) and CdAl (GPT). After 56 days, GOT and GPT plasma activities were reduced in the Cd group. No alteration in plasma levels of creatinine, total bilirubin, and total protein were observed. GOT liver activity increased in the Cd group. No alteration was observed in superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), and malondialdehyde (MDA) dosage. In the Cd group, hepatocyte proportion decreased and sinusoid capillary proportion increased. In the Al and CdAl groups, the nuclear proportion increased and the cytoplasmic proportion decreased. The hepatocyte nucleus density reduced in Cd and increased in the Al group. After 56 days, there was no alteration in the Cd group. In Al and CdAl groups, the nuclear proportion increased without cytoplasmic proportion variation, but the sinusoid capillary proportion was reduced. The hepatocyte nucleus density decreased in the Cd group and increased in the Al and CdAl groups. In conclusion, the liver function indicators showed that A. lappa protected the liver against cadmium toxicity damage.


Liver Cadmium A. lappa Plasma parameters Stereology Oxidative stress 



This work was supported by grants and scholarships from the Fundação de Amparo à Pesquisa do Estado de São Paulo (grant number 2006 ⁄06142-8).

Conflict of Interest

The authors declare that there is no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Fabricia de Souza Predes
    • 1
    Email author
  • Maria Aparecida da Silva Diamante
    • 2
  • Mary Ann Foglio
    • 3
  • Camila Almeida Camargo
    • 4
  • Hiroshi Aoyama
    • 4
  • Silvio Cesar Miranda
    • 5
  • Bread Cruz
    • 2
  • Maria Cristina Cintra Gomes Marcondes
    • 2
  • Heidi Dolder
    • 2
  1. 1.Departamento de Ciências BiológicasUniversidade Estadual do Paraná/Campus ParanaguáParanaguáBrazil
  2. 2.Departamento de Biologia Estrutural e FuncionalUniversidade Estadual de CampinasCampinasBrazil
  3. 3.Centro Pluridisciplinar de Pesquisas Químicas, Biológicas e AgráriasUniversidade Estadual de CampinasCampinasBrazil
  4. 4.Departamento de BioquímicaUniversidade Estadual de CampinasCampinasBrazil
  5. 5.Universidade PaulistaCampinasBrazil

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