Biological Trace Element Research

, Volume 156, Issue 1–3, pp 181–187 | Cite as

Arctigenin Exhibits Relaxation Effect on Bronchus by Affecting Transmembrane Flow of Calcium

Article

Abstract

Arctigenin, a lignan extract from Arctium lappa (L.), exhibits anti-inflammation, antioxidation, vasodilator effects, etc. However, the effects of arctigenin on bronchus relaxation are not well investigated. This study aimed to investigate how arctigenin regulates bronchus tone and calcium ion (Ca2+) flow. Trachea strips of guinea pigs were prepared for testing the relaxation effect of arctigenin to acetylcholine, histamine, KCl, and CaCl2, respectively. Furthermore, l-type calcium channel currents were detected by patch–clamp, and intracellular Ca2+ concentration was detected by confocal microscopy. The results showed that arctigenin exhibited relaxation effect on tracheae to different constrictors, and this was related to decreasing cytoplasmic Ca2+ concentration by inhibiting Ca2+ influx partly through l-type calcium channel as well as promoting Ca2+ efflux. In summary, this study provides new insight into the mechanisms by which arctigenin exhibits relaxation effect on bronchus and suggests its potential use for airway disease therapy.

Keywords

Arctigenin Airway smooth muscle Asthma Calcium Patch–clamp Confocal 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Zhenying Zhao
    • 1
  • Yongqiang Yin
    • 2
  • Zengyong Wang
    • 1
  • Runping Fang
    • 1
  • Hong Wu
    • 2
    • 3
  • Min Jiang
    • 1
  • Gang Bai
    • 1
  • Guo’an Luo
    • 1
  1. 1.College of PharmacyNankai UniversityTianjinPeople’s Republic of China
  2. 2.Department of PharmacologyTianjin Medical UniversityTianjinPeople’s Republic of China
  3. 3.Mudanjiang Medical CollegeMudanjiangPeople’s Republic of China

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