Biological Trace Element Research

, Volume 155, Issue 3, pp 423–430 | Cite as

The Effects of Flaxseed Oil on Cadmium-Induced Oxidative Stress in Rats

  • Sedat Karaca
  • Gökhan EraslanEmail author


In the present study, the effects of flaxseed oil on the oxidant–antioxidant system in cadmium intoxication were investigated in rats. Forty-eight male Wistar albino rats were divided into four equal groups (group 1). No treatment was applied to the control group. On the other hand, groups 2, 3, and 4 were administered with 0.1 ml/rat/day (∼500 mg/kg bw) flaxseed oil by gavage into the stomach, 50 ppm of cadmium (∼4 mg/kg bw) in ad libitum drinking water, and 0.1 ml/rat/day flaxseed oil plus 50 ppm of cadmium, respectively, for 30 days. At the end of the study, malondialdehyde and nitric oxide levels and catalase, superoxide dismutase, and glutathione peroxidase activities were measured in blood and tissue (liver, lung, kidney, brain, heart, and testes) samples. While malondialdehyde and nitric oxide levels increased in the group given cadmium compared to the control group; in the meantime, there were some significant changes in antioxidant enzyme activities. These changes were observed, the trends of decrease or increase compared to the control group. There were positive changes in parameters of the group given with flaxseed oil plus cadmium compared to the group receiving cadmium alone, in other words, values were seen coming close to control group. As a result, cadmium exposure caused oxidative damage to erythrocytes and organs at varying rates, while flaxseed oil reduced the severity of cadmium-induced lipid peroxidation. Therefore, it was concluded that flaxseed oil can be used among compounds as a therapeutic agent or food additive for prophylaxis in cadmium intoxication.


Cadmium Flaxseed oil Oxidative stress Rat 



This research (project code: TSY-09-753) was supported by the Research Fund of Erciyes University.

Conflict of Interest

The authors declare that they have no conflict of interest.


  1. 1.
    Rubilar M, Gutiérrez C, Verdugo M, Shene C, Sineiro J (2010) Flaxseed as a source of functional ingredients. J Soil Sci Plant Nutr 10:373–377CrossRefGoogle Scholar
  2. 2.
    Yang W, Fu J, Yu M, Huang Q, Wang D, Xu J, Deng Q, Yao P, Huang F, Liu L (2012) Effects of flaxseed oil on antioxidative system and membrane deformation of human peripheral blood erythrocytes in high glucose level. Lipids Health Dis 11:88PubMedCrossRefGoogle Scholar
  3. 3.
    Kasote DM (2013) Flaxseed phenolics as natural antioxidants. IFRJ 20:27–34Google Scholar
  4. 4.
    Touré A, Xueming X (2010) Flaxseed lignans: source, biosynthesis, metabolism, antioxidant activity, bioactive components, and health benefits. Compr Rev Food Sci F 9:261–269CrossRefGoogle Scholar
  5. 5.
    Nazıroğlu M (2011) TRPM2 cation channels, oxidative stress, and neurological diseases: where are we now? Neurochem Res 36:355–366PubMedCrossRefGoogle Scholar
  6. 6.
    Somogyi A, Rosta K, Pusztai P, Tulassay Z, Nagy G (2007) Antioxidant measurements. Physiol Meas 28:R41–55PubMedCrossRefGoogle Scholar
  7. 7.
    Guéraud F, Atalay M, Bresgen N, Cipak A, Eckl PM, Huc L, Jouanin I, Siems W, Uchida K (2010) Chemistry and biochemistry of lipid peroxidation products. Free Radic Res 44:1098–1124PubMedCrossRefGoogle Scholar
  8. 8.
    Dailiah Roopha P, Padmalatha C (2012) Effect of herbal preparation on heavy metal (cadmium)-induced antioxidant system in female Wistar rats. J Med Toxicol 8:101–107PubMedCrossRefGoogle Scholar
  9. 9.
    Lazarus M, Orct T, Aladrović J, Ljubić BB, Jurasović J, Blanuša M (2011) Effect of selenium pretreatment on antioxidative enzymes and lipid peroxidation in Cd-exposed suckling rats. Biol Trace Elem Res 142:611–622PubMedCrossRefGoogle Scholar
  10. 10.
    Ognjanović BI, Marković SD, Pavlović SZ, Zikić RV, Stajn AS, Saicić ZS (2008) Effect of chronic cadmium exposure on antioxidant defense system in some tissues of rats: protective effect of selenium. Physiol Res 57:403–411PubMedGoogle Scholar
  11. 11.
    Messaoudi I, El Heni J, Hammouda F, Saïd K, Kerkeni A (2009) Protective effects of selenium, zinc, or their combination on cadmium-induced oxidative stress in rat kidney. Biol Trace Elem Res 130:152–161PubMedCrossRefGoogle Scholar
  12. 12.
    Cuypers A, Plusquin M, Remans T, Jozefczak M, Keunen E, Gielen H, Opdenakker K, Nair AR, Munters E, Artois TJ, Nawrot T, Vangronsveld J, Smeets K (2010) Cadmium stress: an oxidative challenge. Biometals 23:927–940PubMedCrossRefGoogle Scholar
  13. 13.
    Nair AR, Degheselle O, Smeets K, Van Kerkhove E, Cuypers A (2013) Cadmium-induced pathologies: where is the oxidative balance lost (or not)? Int J Mol Sci 14:6116–6143PubMedCrossRefGoogle Scholar
  14. 14.
    Dorta DJ, Leite S, DeMarco KC, Prado IM, Rodrigues T, Mingatto FE, Uyemura SA, Santos AC, Curti C (2003) A proposed sequence of events for cadmium-induced mitochondrial impairment. J Inorg Biochem 97:251–257PubMedCrossRefGoogle Scholar
  15. 15.
    Flora SJ, Mittal M, Mehta A (2008) Heavy metal-induced oxidative stress and its possible reversal by chelation therapy. Indian J Med Res 128:501–523PubMedGoogle Scholar
  16. 16.
    Sears ME (2013) Chelation: Harnessing and enhancing heavy metal detoxification-a review. Thescientificworldjo 219840Google Scholar
  17. 17.
    Abdel Moneim AE, Dkhil MA, Al-Quraishy S (2011) The protective effect of flaxseed oil on lead acetate-induced renal toxicity in rats. J Hazard Mater 194:250–255PubMedCrossRefGoogle Scholar
  18. 18.
    Abdel Moneim AE, Dkhil MA, Al-Quraishy S (2011) Effects of flaxseed oil on lead acetate-induced neurotoxicity in rats. Biol Trace Elem Res 144:904–913PubMedCrossRefGoogle Scholar
  19. 19.
    Abdel-Moneim AE, Dkhil MA, Al-Quraishy S (2011) The redox status in rats treated with flaxseed oil and lead-induced hepatotoxicity. Biol Trace Elem Res 143:457–467PubMedCrossRefGoogle Scholar
  20. 20.
    Abdel Moneim AE (2012) Flaxseed oil as a neuroprotective agent on lead acetate-induced monoamineric alterations and neurotoxicity in rats. Biol Trace Elem Res 148:363–370PubMedCrossRefGoogle Scholar
  21. 21.
    Rizwan S, Naqshbandi A, Khan F (2013) Dietary flaxseed oil supplementation mitigates the effect of lead on the enzymes of carbohydrate metabolism, brush border membrane, and oxidative stress in rat kidney tissues. Biol Trace Elem Res 153:279–290PubMedCrossRefGoogle Scholar
  22. 22.
    Winterbourn CC, Hawkins RE, Brian M, Carrell RW (1975) The estimation of red cell superoxide activity. J Lab Clin Med 55:337–341Google Scholar
  23. 23.
    Fairbanks VF, Klee GG (1987) Biochemical aspect of hematology. In: Tietz NW (ed) Fundamentals of Clinical Chemistry, 3rd edn. WB Saunders, Philadelphia, pp 803–806Google Scholar
  24. 24.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMedGoogle Scholar
  25. 25.
    Miller GL (1959) Protein determination for large numbers of samples. Anal Chem 31:964CrossRefGoogle Scholar
  26. 26.
    Yoshioka T, Kawada K, Shimada T, Mori M (1979) Lipid peroxidation in maternal and cord blood and protective mechanism against activated oxygen toxicity in the blood. Am J Obstet Gynecol 135:372–376PubMedGoogle Scholar
  27. 27.
    Ohkawa H, Ohishi N, Yagi K (1978) Reaction of linoleic acid hydroperoxide with thiobarbutiric acid. J Lipid Res 19:1053–1057PubMedGoogle Scholar
  28. 28.
    Luck H (1965) Catalase. In: Bergmeyer H (ed) Methods of Enzymatic Analysis. Academic Press, New York, pp 885–894CrossRefGoogle Scholar
  29. 29.
    Sun Y, Oberley LW, Li Y (1988) A simple method for clinical assay of superoxide dismutase. Clin Chem 34:497–500PubMedGoogle Scholar
  30. 30.
    Paglia PE, Valentine WN (1967) Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 70:158–169PubMedGoogle Scholar
  31. 31.
    Tracey WR, Tse J, Carter G (1995) Lipopolysaccharide-induced changes in plasma nitrite and nitrate concentrations in rats and mice: pharmacological evaluation of nitric oxide synthase inhibitors. J Pharmacol Exp Ther 272:1011–1015PubMedGoogle Scholar
  32. 32.
    Jomova K, Valko M (2011) Advances in metal-induced oxidative stress and human disease. Toxicology 283:65–87PubMedCrossRefGoogle Scholar
  33. 33.
    Thévenod F (2009) Cadmium and cellular signaling cascades: to be or not to be? Toxicol Appl Pharmacol 238:221–239PubMedCrossRefGoogle Scholar
  34. 34.
    Xu B, Chen S, Luo Y, Chen Z, Liu L, Zhou H, Chen W, Shen T, Han X, Chen L, Huang S (2011) Calcium signaling is involved in cadmium-induced neuronal apoptosis via induction of reactive oxygen species and activation of MAPK/mTOR network. PLoS One 6:e19052PubMedCrossRefGoogle Scholar
  35. 35.
    Garcia JJ, Martínez-Ballarín E, Millán-Plano S, Allué JL, Albendea C, Fuentes L, Escanero JF (2005) Effects of trace elements on membrane fluidity. J Trace Elem Med Biol 19:19–22PubMedCrossRefGoogle Scholar
  36. 36.
    El-Beltagi HS, Salama ZA, El-Hariri DM (2007) Evaluation of fatty acids profile and the content of some secondary metabolites in seeds of different flax cultivars (Linum Usitatissimum L.). Gen Appl Plant Physiology 33:187–202Google Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.College of Health Science, Department of Pharmacology and ToxicologyErciyes UniversityKayseriTurkey
  2. 2.Faculty of Veterinary Medicine, Department of Pharmacology and ToxicologyErciyes UniversityKayseriTurkey

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